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Study on a factor in silkglands that suppresses defense responses of plants during infestation

Research Project

Project/Area Number 23580151
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionYamaguchi University

Principal Investigator

MATSUI Kenji  山口大学, 医学(系)研究科(研究院), 教授 (90199729)

Co-Investigator(Kenkyū-buntansha) OZAWA Rika  京都大学, 生態学研究センター, 研究員 (90597725)
KOBAYASHI Jun  山口大学, 農学部, 教授 (70242930)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsみどりの香り / カイコ / 絹糸腺 / リポキシゲナーゼ / 植物昆虫相互作用 / 脂肪酸ヒドロペルオキシド脱水酵素 / カイコガ / ケト脂肪酸 / 鱗翅目昆虫
Research Abstract

We found an activity to suppress formation of green leaf volatiles in the silk gland of silkworms. After purification, we obtained a protein of 31 kDa, that showed high fatty acid hydroperoxide dehydratase activity. When various isomers of substrates were used, the enzyme showed very strict substrate specificity to 13S-ZE isomer. In fact, this isomer is the one accountable to formation of green leaf volatiles in plants. From these observation, it was suggested that the enzyme is not only the antioxidative enzyme but probably involved in oxylipin metabolism either in plants or in the insects. When we analyzed the gene encoding the enzyme, we found it was a gene that had not been assigned. We are now trying to get a transgenic silkworm that has deletion of the gene, thereafter, we will be able to see the physiological and ecological significances of the enzyme activity for silkworms.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (10 results)

All 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (4 results) Remarks (3 results)

  • [Journal Article] Formation of 1-octen-3-ol from Aspergillus flavus conidia is accelerated after disruption of cells independently of Ppo oxygenases, and is not a main cause of inhibition of germination2014

    • Author(s)
      Kana Miyamoto, Tomoko Murakami, Pattana Kakumyan, Nancy P. Keller, Kenji Matsui
    • Journal Title

      PeerJ

      Volume: 2 Pages: e395-e395

    • DOI

      10.7717/peerj.395

    • NAID

      120005671052

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intake and transformation to a glycoside of (Z)-3-hexenol from infested neighbors reveals a mode of plant odor reception and defense2014

    • Author(s)
      Koichi Sugimoto, Kenji Matsui, Yoko Iijima, Yoshihiko Akakabe, Shoko Muramoto, Rika Ozawa, Masayoshi Uefune, Ryosuke Sasaki, Kabir Md. Alamgir, Shota, Akitake, Tatsunori Nobuke, Ivan Galis, Koh Aoki, Daisuke Shibata, Junji Takabayashi
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 111 Pages: 7144-7149

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Traumatin- and dinortraumatin-containing galactolpids in Arabidopsis. Their formation in tissue-disrupted leaves as counterparts of green leaf volatiles2013

    • Author(s)
      Anna Nakashima, Stephan H. von Reuss, Hiroyuki Tasaka, Misaki Nomura, Satoshi Mochizuki, Yoko Iijima, Koh Aoki, Daisuke Shibata, Wilhelm Boland, Junji Takabayashi, Kenji Matsui
    • Journal Title

      J. Biol. Chem

      Volume: 288 Pages: 26078-26088

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Purification and characterization of a factor suppressing green leaf volatile formation in silk gland of silkworms2013

    • Author(s)
      Hiroki Takai, Saki Fujii, Rika Ozawa, Hideo Dohra, Tosshiyuki Ohnishi, Jun Kobayashi, Junji Takabayashi, Kenji Matsui
    • Organizer
      5^<th> Asian Symposium on Plant Lipids
    • Place of Presentation
      Gwangju, Korea
    • Year and Date
      2013-11-29
    • Related Report
      2013 Final Research Report
  • [Presentation] カイコ絹糸腺のみどりの香り精製抑制因子の精製とその性質に着いて2013

    • Author(s)
      高井嘉樹、藤井沙季、小澤理香、道羅英夫、大西利幸、小林淳、高林純示、松井健二
    • Organizer
      日本農芸化学会支部合同広島大会
    • Place of Presentation
      県立広島大学広島キャンパス,広島市
    • Year and Date
      2013-09-05
    • Related Report
      2013 Final Research Report
  • [Presentation] カイコ絹糸腺のみどりの香り生成抑制因子の精製とその性質について2013

    • Author(s)
      高井嘉樹、藤井沙季、小澤理香、道羅英夫、大西利幸、小林淳、高林純示、松井健二
    • Organizer
      日本農芸化学会関西・中四国・西日本支部合同広島大会
    • Place of Presentation
      県立広島大学広島キャンパス(広島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Purification and characterization of a factor suppressing green leaf volatile formation in silk gland of silkworms2013

    • Author(s)
      H. Takai, S. Fujii, R. Ozawa, H. Dohra, T. Ohnishi, J. Kobayashi, J. Takabayashi, K. Matsui
    • Organizer
      5th Asian Symposium on Plant Lipids
    • Place of Presentation
      Gwangju, Korea
    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://web.cc.yamaguchi-u.ac.jp/~matsui/

    • Related Report
      2013 Final Research Report
  • [Remarks] Matsui Koeduka Laboratory

    • URL

      http://web.cc.yamaguchi-u.ac.jp/~matsui/

    • Related Report
      2013 Annual Research Report
  • [Remarks] Matsui Kenji Laboratory (Yamaguchi University)

    • URL

      http://web.cc.yamaguchi-u.ac.jp/~matsui/

    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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