The elucidation of the new high dimension function of glucosamine.
| Project/Area Number |
23580183
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAGAOKA Isao 順天堂大学, 医学部, 教授 (60164399)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | グルコサミン / 骨芽細胞 / 軟骨細胞 / サーチュイン / 標的遺伝子 / 軟骨代謝 / 骨代謝 / グリコサミノグリカン |
|---|
| Research Abstract |
GlcN may have a potential not only to induce the osteoblastic cell differentiation especially at middle-late stages, but also to suppress the osteoclastic cell differentiation, thereby possibly increasing bone matrix deposition and decreasing bone resorption, and eventually modulate bone metabolism in OA.
Next, we searched the target genes in chondrocyte cell line by incubating in the presence of GlcN. RT-PCR analyses revealed that GlcN significantly increased the sirtuin (SIRT) 1 mRNA expression levels. Furthermore, western blot analyses revealed that GlcN significantly increased the protein level of SIRT1. Moreover, GlcN did not essentially affect the SIRT1 expression levels in osteoblastic cells. GlcN is able to upregulate the mRNA and protein levels of SIRT1 in chondrocytes, thereby possibly exhibiting the protective action on OA.
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Report
(4 results)
Research Products
(29 results)
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[Book] Effect of glucosamine, a therapeutic agent for osteoarthritis, on osteoblastic cell differentiation. Proceedings of the 9th Asia Pacific Chitin and Chitosan Symposium (Nha Trang, Vietnam, Aug 3-6, 2011), Edited by Ngo ND, Vårum KM, Nguyen DA, Trang TS.2012
Author(s)
Igarashi M, Sakamoto K, Nagaoka I.
Publisher
Agricultural Publishing House
Related Report
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