A development of near infrared imaging spectroscopy with heavy water to visualise water movement in plants

Research Project

Project/Area Number	23580355
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Agricultural information engineering
Research Institution	Iwate University
Principal Investigator	MATSUSHIMA Uzuki 岩手大学, 農学部, 准教授 (70315464)
Co-Investigator(Kenkyū-buntansha)	SHONO Hiroshi 岩手大学, 農学部, 准教授 (90235721)
Project Period (FY)	2011 – 2013
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000) Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	植物水分生理 / 蒸散 / 可視化情報 / 重水 / トレーサー / 近赤外イメージング / 植物細胞観察 / 透過型イメージング / トレーサ / 透過型
Research Abstract	Because the chemical characteristics is similar to water, deuterium oxide (D2O) well up-taken in plants can be a good tracer to study plant water relations. At a specific near infrared waveband, near-infrared is absorbed not by D2O but by water. Therefore, a combination of near-infrared imaging and D2O tracer provides a method observing water movement in plants. In this study, we established macroscopic and microscopic visualization methods to investigate water movement in plants. We also developed an user interface for this novel method.