The analyses of glomerular filtration system and epithelial barrier using genetically-engineered mice
Project/Area Number |
23590242
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General anatomy (including Histology/Embryology)
|
Research Institution | Dokkyo Medical University |
Principal Investigator |
Itoh Masahiko 獨協医科大学, 医学部, 准教授 (70270486)
|
Project Period (FY) |
2011-04-28 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | 腎臓 / 糸球体 / 糸球体上皮細胞 / 密着結合 / 糸球体障害 / 蛋白尿 / ZO-1 / Tjp1 / 血液濾過 / タンパク尿 / 足細胞 / スリット膜 / 足突起 / 濾過 |
Outline of Final Research Achievements |
Recent studies have demonstrated that podocytes in the kidney glomerulus play a crucial role in blood filtration and in the pathogenesis of proteinuria and glomerular sclerosis; however, the molecular mechanisms that organize the podocyte filtration barrier are not fully understood. We suggest that tight junction protein 1 (Tjp1 or ZO-1), which is encoded by Tjp1 gene, plays an essential role in establishing the podocyte filtration barrier. The podocyte-specific deletion of Tjp1 down-regulated the expression of podocyte membrane proteins, impaired the interdigitation of the foot processes and the formation of the slit diaphragm, resulting in glomerular dysfunction. We found the possibility that podocyte filtration barrier requires the integration of two independent units, the pre-existing epithelial junction components and the newly synthesized podocyte-specific components, at the final stage in glomerular morphogenesis, for which Tjp1 is indispensable.
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Report
(6 results)
Research Products
(10 results)