| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Research Abstract |
Chromaffin cells were enzymatically dissociated from adrenal medulla from male C57BL mice. By use of amperometry, single exocytotic events were analyzed in single mouse chromaffin cells with a carbon fiber microelectrode. To characterize a single exocytotic event detected as a single current spike, the spike parameters and the frequency of events were analyzed. To clarify the implication of the interaction of syntaxin 1A and CaMKII in the regulation of exocytosis, we examined the events frequency and the kinetics of single events in the cells prepared from R151G syntaxin 1A knock-in mice. The significant reduction was observed in the frequency of exocytotic events evoked by acetylcholine in knock-in cells. The frequency distribution of the spike height, middle width and area were slightly shifted to smaller values in knock-in cells. Syntaxin 1A knock-in seemed to affect not only the event frequency but also the kinetics of the fusion process.
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