Functional analyses of large Maf transcription factors in the development of pancreatic endocrine cells and its application

• Project/Area Number: 23590325
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: University of Tsukuba
• Principal Investigator: OISHI Hisashi 筑波大学, 医学医療系, 准教授 (30375513)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Satoru 筑波大学, 医学医療系, 教授 (50271896)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: インシュリン / β細胞 / 大Maf群転写因子 / マウス / 糖尿病 / インスリン / 生物発光

Research Abstract

It is an important research subject to understand the mechanisms of pancreatic beta-cell development and maturation for the achievement of regeneration therapy for diabetes. The project yielded these three results: (1) To analyze the function of large Maf transcription factors in pancreatic endocrine cells, we generated floxed MafB mice. (2) To monitor the newly induced beta-like cells in mouse liver, we established the in vivo imaging system capable to quantify the insulin gene activity (3) Dominant role of MafA in the beta-like cell conversion in comparison with MafB.

Research Products

• [Journal Article] Bioluminescence imaging of β cells and intrahepatic insulin gene activity under normal and pathological conditions (2014)
  • Author(s): Katsumata T, Oishi H, Sekiguchi Y, Nagasaki H, Daassi D, Tai PH, Ema M, Kudo T, Takahashi S
  • Journal Title: Plos One Volume: -
  • Peer Reviewed
• [Journal Article] In vivo monitoring of pancreatic β -cell mass and intrahepatic insulin gene activity in Ins1-luc BAC transgenic mice by bioluminescence imaging (2013)
  • Author(s): Katsumata T, Oishi H, Sekiguchi Y, Nagasaki H, Daassi D, Ema M, Kudo T, Takahashi S
  • Journal Title: Plos One Volume: 8 Issue: 4 Pages: e60411-e60411
  • DOI: 10.1371/journal.pone.0060411
  • Peer Reviewed
• [Journal Article] A bacterial artificial chromosome transgene with polymorphic Cd72 inhibits the development of glomerulonephritis and vasculitis in MRL-Faslpr lupus mice (2013)
  • Author(s): Oishi, H., Tsubaki, T., Miyazaki, T., Ono, M., Nose, M. and Tahahashi, S.
  • Journal Title: Journal of Immunology Volume: 190 Issue: 5 Pages: 2129-2137
  • DOI: 10.4049/jimmunol.1202196
  • Peer Reviewed
• [Journal Article] Delayed cutaneous wound healing in Fam129b/Minerva-deficient mice. (2012)
  • Author(s): Oishi H et al.
  • Journal Title: J. Biochem Volume: 152 Issue: 6 Pages: 549-555
  • DOI: 10.1093/jb/mvs100
  • Peer Reviewed
• [Journal Article] Sekiguchi Y, Owada J, Oishi H, Katsumata T, Ikeda K, Kudo T, Takahashi S (2012)
  • Author(s): Noninvasive monitoring ofβ-cell mass and fetalβ-cell genesis in mice using bioluminescence imaging
  • Journal Title: Exp Anim Volume: 61(4) Pages: 445-451
  • Peer Reviewed
• [Journal Article] Colmegna I, Pryshchep S, Oishi H, Goronzy JJ, Weyand CM (2012)
  • Author(s): Dampened ERK signaling in hematopoietic progenitor cells in rheumatoid arthritis
  • Journal Title: Clin Immunol Volume: 143(1) Issue: 1 Pages: 73-82
  • DOI: 10.1016/j.clim.2012.01.007
  • Peer Reviewed
• [Journal Article] The E3 ubiquitin ligase activity of Trip12 is essential for mouse embryogenesis (2011)
  • Author(s): Kajiro M, Tsuchiya M, Kawabe Y, Furumai R, Iwasaki N, Hayashi Y, Katano M, Nakajima Y, Goto N, Watanabe T, Murayama A, Oishi H, Ema M, Takahashi S, Kishimoto H, Yanagisawa J
  • Journal Title: PLoS One Volume: 6(10) Issue: 10 Pages: e25871-e25871
  • DOI: 10.1371/journal.pone.0025871
  • Peer Reviewed
• [Presentation] 生物発光イメージングを用いた膵β細胞およ肝内インスリン遺伝子発現の可視化 (2014)
  • Author(s): 大石久史, 勝又斗紀夫, Tai Peihan, Jung Yunshin, 関口有佳里, 高橋智
  • Organizer: 第119回日本解剖学会総会・全国学術大会
  • Place of Presentation: 自治医科大学キャンパス, 栃木県下野市
  • Year and Date: 2014-03-29
• [Presentation] 生物発光イメージングを用いた膵β細胞および肝内インスリン遺伝子発現の可視化 (2014)
  • Author(s): 大石久史、勝又斗紀夫、Tai Peihan、Jung Yunshin、関口有佳里、高橋智
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 自治医科大学キャンパス（栃木県下野市）
• [Presentation] 生物発光イメージングを用いた膵β細胞およ肝内インスリン遺伝子発現の可視化 (2013)
  • Author(s): 大石久史, 勝又斗紀夫, 関口有佳里, Daasi D,依馬正次, 工藤崇, 高橋智
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: つくば国際会議場, 茨城県つくば市
  • Year and Date: 2013-05-15
• [Presentation] 膵内分泌細胞発生・分化における大Maf群転写因子の役割とその応用 (2013)
  • Author(s): 大石久史, 工藤崇, 高橋智
  • Organizer: 第118回日本解剖学会総会・全国学術大会
  • Place of Presentation: サンポートホール高松・かがわ国際会議場, 香川県高松市
  • Year and Date: 2013-03-29
• [Presentation] 生物発光イメージングを用いた膵β細胞および肝内インスリン遺伝子発現の可視化 (2013)
  • Author(s): 大石久史、勝又斗紀夫、関口有佳里、Dhouha Daassi、依馬正次、高橋智
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
• [Presentation] Direct Conversion of Mouse Liver Cells to Insulin-producing Cells by Defined Factors (2013)
  • Author(s): Takahiro Itagaki, Hisashi Oishi, Yutaro Itoh, Pei-Han Tai, Masami Ojima, Satoru Takahashi
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
• [Presentation] 膵内分泌細胞発生・分化における大Maf群転写因子の役割とその応用 (2013)
  • Author(s): 大石 久史、工藤 崇、高橋 智
  • Organizer: 第118回日本解剖学会総会・全国学術大会
  • Place of Presentation: 香川県高松市
  • Invited
• [Presentation] In vivo monitoring of pancreaticbe-cell mass and intrahepatic insulin gene activity in ins1-luc BAC transgenic mice by bioluminescence imaging (2012)
  • Author(s): Katsumata T, Oishi H, Sekiguchi Y, Nagasaki H, Daassi D, Ema M, Kudo T, Takahashi S
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場, 福岡県福岡市
  • Year and Date: 2012-12-13
• [Presentation] Bioluminescence monitoring of intrahepatic beta-like cells show distinct roles for transcription factors, MafA and MafB on the generation of insulin-producing/secreting cells in vivo (2012)
  • Author(s): Nagasaki H, Oishi H, Katsumata T, Sekiguchi Y, Kudo T, Takahashi S
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場, 福岡県福岡市
  • Year and Date: 2012-12-11
• [Presentation] Bioluminescence imaging of novel BAC transgenic mice expressing luciferase reporter under the control of the insulin locus (2012)
  • Author(s): Oishi H, Katsumata T, Sekiguchi Y, Nagasaki H, Ema M, Takahashi S
  • Organizer: 第72回米国糖尿病学会
  • Place of Presentation: フィラデルフィアコンベンションセンター, 米国フィラデルフィア
  • Year and Date: 2012-06-10
• [Presentation] In vivo monitoring of intrahepatic insulin gene activity by bioluminescence imaging (2011)
  • Author(s): Sekiguchi Y, Oishi H, Katsumata T, Kudo T, Takahashi S
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜, 神奈川県横浜市
  • Year and Date: 2011-12-13
• [Presentation] Bioluminescence imaging of novel BAC transgenic mice expressing luciferase reporter gene under the control of the insulin locus (2011)
  • Author(s): Katsumata T, Oishi H, Sekiguchi Y, Ema M, Kudo T, Takahashi S
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜, 神奈川県横浜市
  • Year and Date: 2011-12-13
• [Presentation] Role of Large Maf transcription factors in pancreatic endocrine cell development (2011)
  • Author(s): Oishi H, Ogata K, Kudo T, Takahashi S
  • Organizer: 3rd Beta Cell Workshop
  • Place of Presentation: LO-Skolen, デンマーク
  • Year and Date: 2011-10-25
• [Presentation] In vivo monitoring of intrahepatic insulin gene activity by bioluminescence imaging (2011)
  • Author(s): Yukari Sekiguchi, Hisashi Oishi, Tokio Katsumata, Takashi Kudo, Satoru Takahashi
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] Bioluminescence imaging of novel BAC transgenic mice expressing luciferase reporter gene under the control of the insulin locus (2011)
  • Author(s): Tokio Katsumata, Hisashi Oishi, Yukari Sekiguchi, Masatsugu Ema, Takashi Kudo, Satoru Takahashi
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] Role of Large Maf transcription factors in pancreatic endocrine cell development (2011)
  • Author(s): Hisashi Oishi, Kiyohito Ogata, Takashi Kudo, Satoru Takahashi
  • Organizer: Beta cell workshop
  • Place of Presentation: LO-Skolen（デンマーク）
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/basic-med/anatomy/embryology/