Identification and the elucidation of the mechanism of interleukin 18 induced metastasis inhibitory factor(s)

• Project/Area Number: 23590480
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Hyogo Medical University
• Principal Investigator: YAMADA Naoko 兵庫医科大学, 医学部, 講師 (10319858)
• Co-Investigator(Kenkyū-buntansha): TERADA Nobuyuki 兵庫医科大学, 医学部, 名誉教授 (50150339) ; NAKASHO Keiji 兵庫医科大学, 医学部, 教授 (00217712) ; OHYAMA Hideki 兵庫医科大学, 医学部, 准教授 (90280685) ; YAMANEGI Koji 兵庫医科大学, 医学部, 講師 (00434944)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: インターロイキン18 / 転移 / 骨肉腫 / インターロイキン

Research Abstract

We showed the possibility of the presence of IL-18-induced metastasis inhibitory factor(s) in the previous paper. The purpose of this research is to identify the factor(s) and to clarify its metastasis suppression mechanism. We revealed that this factor mainly existed in serum. We found 32 candidates of the factor(s) using the antibody arrays. As a result of investigating the metastasis suppressing activity of five factors with high expression level, three of them inhibited the tumor cell invasion. Further, we found 42 candidates of the factor(s) through two-dimensional electrophoresis and the MALDI-TOF-MS.

Research Products

• [Journal Article] Estrogen decreases the expression of claudin-5 in vascular endothelial cells in the murine uterus. (2014)
  • Author(s): Hata M, Yamanegi K, Yamada N, Ohyama H, Yukitatsu Y, Nakasho K, Okamura H, Terada N.
  • Journal Title: Endocr J Volume: -
  • NAID: 130004443978
  • Peer Reviewed
• [Journal Article] Decreased expression of VE-cadherin and claudin-5 and increased phosphorylation of VE-cadherin in vascular endothelium in nasal polyps. (2013)
  • Author(s): Yukitatsu Y, Hata M, Yamanegi K, Yamada N, Ohyama H, Nakasho K, Kojima Y, Oka H, Tsuzuki K, Sakagami M, Terada N.
  • Journal Title: Cell Tissue Res Volume: 352 Issue: 3 Pages: 647-657
  • DOI: 10.1007/s00441-013-1583-0
  • Peer Reviewed
• [Journal Article] Hypoxia downregulates the expression of cell surface MICA without increasing soluble MICA in osteosarcoma cells in a HIF-1α-dependent manner. (2012)
  • Author(s): Yamada N, Yamanegi K, Ohyama H, Hata M, Nakasho K, Futani H, Okamura H, Terada N.
  • Journal Title: Int J Oncol. Volume: 41(6) Issue: 6 Pages: 2005-2012
  • DOI: 10.3892/ijo.2012.1630
  • Peer Reviewed
• [Journal Article] Downregulation of matrix metalloproteinase-9 mRNA by valproic acid plays a role in inhibiting the shedding of MHC class I-related molecules A and B on the surface of human osteosarcoma cells. (2012)
  • Author(s): Yamanegi K, Yamane J, Kobayashi K, Ohyama H, Nakasho K, Yamada N, Hata M, Fukunaga S, Futani H, Okamura H, Terada N.
  • Journal Title: Oncol Rep. Volume: 28(5) Issue: 5 Pages: 1585-1590
  • DOI: 10.3892/or.2012.1981
  • Peer Reviewed
• [Journal Article] Valproic acid cooperates with hydralazine to augment the susceptibility of human osteosarcoma (2012)
  • Author(s): Yamanegi K, Yamane J, Kobayashi K, Kato-Kogoe N, Ohyama H, Nakasho K, YamadaN, Hata M, Fukunaga S, Futani H, Okamura H, Terada N.
  • Journal Title: cells to Fas- and NK cell-mediated cell death.Int J Oncol. Volume: 41(1) Pages: 83-91
  • DOI: 10.3892/ijo.2012.1438
  • Peer Reviewed
• [Journal Article] The promotional effect of IL-22 on mineralization activity of periodontal ligament cells (2012)
  • Author(s): Kato-Kogoe N, Nishioka T, Kawabe M, Kataoka F, Yamanegi K, Yamada N, Hata M, Yamamoto T, Nakasho K, Urade M, Terada N, Ohyama H
  • Journal Title: Cytokine Volume: 59(1) Issue: 1 Pages: 41-8
  • DOI: 10.1016/j.cyto.2012.03.024
  • Peer Reviewed
• [Journal Article] Phenotypic characteristics and proliferative activity of hyperplastic ductile cells in cholangiofibrosis induced by thioacetamide in rats. Epub ahead of print (2011)
  • Author(s): Hata M, Iida H, Yamanegi K, Yamada N, Ohyama H, Hirano H, Nakasho K, Terada N.
  • Journal Title: Exp Toxicol Pathol. Volume: -
  • Peer Reviewed
• [Journal Article] A case of mixed ductal-endocrine carcinoma of the pancreas. (2011)
  • Author(s): Hirano H, Terada N, Yamada N, Yamanegi Y, Oyama H, Nishigami T, Nakasho K.
  • Journal Title: Med Mol Morphol. Volume: 44 Pages: 58-62
  • NAID: 10029036648
  • Peer Reviewed
• [Presentation] 低酸素による細胞表面MICAの糖鎖修飾の低下 (2013)
  • Author(s): 山田直子、山根木康嗣、大山秀樹、寺田信行、中正恵二
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-03
• [Presentation] 骨肉腫細胞における低酸素誘導性の細胞表面MICAの発現低下 (2012)
  • Author(s): 山田直子、山根木康嗣、大山秀樹、中正恵二、秦正樹、寺田信行
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡
• [Presentation] ヒストン脱アセチル化阻害剤を用いた腫瘍血管新生抑制 (2012)
  • Author(s): 山根木康嗣、小林健太、中正恵二、大秀樹、山田直子、秦正樹、寺田信行
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] Th17細胞産生性サイトカインが血管石灰化に及ぼす影響 (2012)
  • Author(s): 大山秀樹、片岡芙紗、川辺睦記、小越菜保子、山根木康嗣、山田直子、秦正樹、中正恵二、寺田信行
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] 骨肉腫細胞における低酸素誘導性の細胞表面MICAの発現低下 (2012)
  • Author(s): 山田 直子、山根木 康嗣、大山 秀樹、中正 恵二、秦 正樹、寺田 信行
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡
• [Presentation] 骨肉腫に対するIL-18とイホマイドの併用療法の可能性 (2011)
  • Author(s): 山田直子、秦正樹、山根木康嗣、大山秀樹、中正恵二、麩谷博之、岡村春樹、寺田信行
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] Epigenetic drugによるヒト骨肉腫細胞の抗腫瘍免疫細胞に対する感受性亢進 (2011)
  • Author(s): 山根木康嗣、小林健太、大山秀樹、秦正樹、山田直子、中正恵二、寺田信行
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
• [Presentation] IL12RB2転写活性の個体差におけるAP-1ファミリー転写因子の関与 (2011)
  • Author(s): 大山秀樹、小越菜保子、山根木康嗣、山田直子、秦正樹、片岡芙紗、中正恵二、寺田信行
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
• [Presentation] 骨肉腫に対するIL-18とイホマイドの併用療法の可能性 (2011)
  • Author(s): 山田直子、秦正樹、山根木康嗣、大山秀樹、中正恵二、麩谷博之、岡村春樹、寺田信行
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] pigenetic drugによるヒト骨肉腫細胞の抗腫瘍免疫細胞に対する感受性亢進 (2011)
  • Author(s): 山根木康嗣, 小林健太, 大山秀樹, 秦正樹, 山田直子, 中正恵二, 寺田信行
  • Organizer: 日本病理学会総会
  • Place of Presentation: 横浜