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Analysis of virus replication mechanism using minigenome or recombinant virus system

Research Project

Project/Area Number 23590539
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Virology
Research InstitutionWakayama Medical University

Principal Investigator

NISHIO Machiko  和歌山県立医科大学, 医学部, 教授 (70156040)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsパラインフルエンザウイルス2型 / リコンビナントウイルス / ミニゲノムシステム / V蛋白 / ウイルス増殖機構 / caspase-1 / tetherin / 増殖機構 / パラミクソウイルス / ウイルス増殖 / ミニゲノム
Research Abstract

To search for host factors interacting with human parainfluenza virus type 2 (hPIV2) V protein systematically, I used the yeast two-hybrid system, and identified some proteins. Tetherin is a cellular membrane protein that restricts budding of a number of enveloped viruses. I demonstrated that tetherin interacts with V protein, and inhibits hPIV2 growth. The V protein inhibits the caspase-1-mediated maturation of IL-1beta via its interaction with caspase-1. Moreover, it interacts with adaptor-related protein complex 1 (AP-1) that is important for apical sorting of hPIV2.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (31 results)

All 2013 2012 2011

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (23 results)

  • [Journal Article] Full conversion of the hemagglutinin-neuraminidase specificity of the parainfluenza virus 5 fusion protein by replacement of 21 amino acids in its head region with those of the simian virus 41 fusion protein2013

    • Author(s)
      Tsurudome M, Nakahashi M, Matsushima Y, Ito M, Nishio M, Kawano M, Komada H, Nosaka T
    • Journal Title

      J . Virol

      Volume: 87 Pages: 8342-8350

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human parainfluenza virus type 2 V Protein inhibits TRAF6-mediated ubiquitination of IRF7 to prevent TLR7- and TLR9-dependent interferon induction2013

    • Author(s)
      Kitagawa Y, Yamaguchi M, Zhou M, Nishio M, Itoh M, Gotoh B
    • Journal Title

      J . Virol

      Volume: 87 Pages: 7966-7976

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Full conversion of the hemagglutinin-neuraminidase specificity of the parainfluenza virus 5 fusion protein by replacement of 21 amino acids in its head region with those of the simian virus 41 fusion protein.2013

    • Author(s)
      Tsurudome M, Nakahashi M, Matsushima Y, Ito M, Nishio M, Kawano M, Komada H, Nosaka T.
    • Journal Title

      J. Virol.

      Volume: 87 Issue: 15 Pages: 8342-8350

    • DOI

      10.1128/jvi.03549-12

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human Parainfluenza Virus Type 2 V Protein Inhibits TRAF6-Mediated Ubiquitination of IRF7 To Prevent TLR7- and TLR9-Dependent Interferon Induction.2013

    • Author(s)
      Kitagawa Y, Yamaguchi M, Zhou M, Nishio M, Itoh M, Gotoh B.
    • Journal Title

      J. Virol.

      Volume: 87 Issue: 14 Pages: 7966-7976

    • DOI

      10.1128/jvi.03525-12

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A tryptophan-rich motif in the human parainfluenza virus type 2 V protein is critical for the blockade of toll-like receptor 7 (TLR7)- and TLR9-dependent signaling2011

    • Author(s)
      Kitagawa Y, Yamaguchi M, Zhou M, Komatsu T, Nishio M, Sugiyama T, Takeuchi K, Itoh M, Gotoh B
    • Journal Title

      J . Virol

      Volume: 85 Pages: 4606-4611

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of domains on the fusion (F) protein trimer that influence the hemagglutinin-neuraminidase specificity of the F protein in mediating cell-cell fusion2011

    • Author(s)
      Tsurudome M, Ito M, Nishio M, Nakahashi M, Kawano M, Komada H, Nosaka T, Ito Y
    • Journal Title

      J . Virol

      Volume: 85 Pages: 3153-3161

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of domains on the F protein trimer that influence the hemagglutinin-neuraminidase specificity of the F protein in mediating cell-cell fusion.2011

    • Author(s)
      Tsurudome M, Ito M, Nishio M, Nakahashi M, Kawano M, Komada H, Nosaka T, Ito Y.
    • Journal Title

      Journal of Virology

      Volume: 85 Pages: 3153-3161

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A tryptophan-rich motif in hPIV2 V protein is critical for the blockade of toll-like receptor 7 (TLR7)-and TRL9-dependent signaling.2011

    • Author(s)
      Kitagawa Y, Yamaguchi M, Zhou M, Komatsu T, Nishio M, Sugiyama T, Tadeuchi K, Itoh M, Gotoh B.
    • Journal Title

      Journal of Virology

      Volume: 85 Pages: 4606-4611

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒトパラインフルエンザウイルス2型(hPIV2)V蛋白と結合する宿主細胞蛋白AP1M1及びAP1M2がウイルス増殖に与える影響2013

    • Author(s)
      西尾真智子、太田圭介、湯峰奈都子、鶴留雅人、五藤秀男
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒトパラインフルエンザウイルス2型(hPIV2)とTetherin(BST-2)の相互作用2013

    • Author(s)
      太田圭介、五藤秀男、湯峰奈都子、西尾真智子
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒトパラインフルエンザウイルス2型の感染によるA型インフルエンザウイルスの増殖促進2013

    • Author(s)
      五藤秀男、太田圭介、湯峰奈都子、西尾真智子
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] パラインフルエンザウイルスのHN蛋白とF蛋白の機能的相互作用の解析2013

    • Author(s)
      鶴留雅人、伊藤守弘、西尾真智子、河野光雄、駒田洋、大塚順平、野阪哲哉
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] Kolakofsky D Human parainfluenza virus type 2 V protein inhibits activity of caspase-12013

    • Author(s)
      Nishio M, OhtanK, Tsurudome M
    • Organizer
      XV International conference on Negative Strand Viruses
    • Place of Presentation
      グラナダ、スペイン
    • Related Report
      2013 Final Research Report
  • [Presentation] Interaction between human parainfluenza virus type 2 V protein and tetherin, an antiviral host factor2013

    • Author(s)
      Ohta K, Goto H, Yumine N, Nishio M
    • Organizer
      XV International conference on Negative Strand Viruses
    • Place of Presentation
      グラナダ、スペイン
    • Related Report
      2013 Final Research Report
  • [Presentation] Conversion of the parainfluenza virus 5 F protein to a simian virus 41 HN-specfic protein by amino acid substitutions2013

    • Author(s)
      Tsurudome M, Ito M, Nishio M, Nosaka T
    • Organizer
      XV International conference on Negative Strand Viruses
    • Place of Presentation
      グラナダ、スペイン
    • Related Report
      2013 Final Research Report
  • [Presentation] 貝類から分離したウェルシュ菌の解析2013

    • Author(s)
      湯峰奈都子、太田圭介、西尾真智子
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒトパラインフルエンザウイルス2型(hPIV2)V蛋白と結合する宿主蛋白AP1M1及びAP1M2がウイルス増殖に与える影響2013

    • Author(s)
      西尾真智子、太田圭介、湯峯奈都子、鶴留雅人、五藤秀男
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] パラインフルエンザウイルスのHN蛋白とF蛋白の機能的相互作用の解析2013

    • Author(s)
      鶴留雅人、伊藤守弘、西尾真智子、河野光雄、駒田洋、大塚順平、野坂哲哉
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] パラインフルエンザウイルス2型の感染によるA型インフルエンザウイルスの増殖促進2013

    • Author(s)
      五藤秀男、太田圭介、湯峯奈都子、西尾真智子
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] パラインフルエンザウイルス2型(hPIV-2)とTetherin(BST-2)の相互作用2013

    • Author(s)
      太田圭介、五藤秀男、湯峯奈都子、西尾真智子
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] 貝類から分離したウェルシュ菌の解析2013

    • Author(s)
      湯峰奈都子、太田圭介、西尾真智子
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒトパラインフルエンザウイルス2型(hPIV2)V蛋白によるcaspase-1活性化抑制2012

    • Author(s)
      西尾真智子、太田圭介、湯峰奈都子、五藤秀男
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report
  • [Presentation] TLR7/9依存性インターフェロン産生抑制におけるV蛋白質とTRF6の相互作用の重要性2012

    • Author(s)
      北川善紀、山口まゆ、小松孝行、周敏、竹内健司、西尾真智子、伊藤正恵、後藤敏
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 分子学的手法を用いた食肉中の腸管病原性C.perfringensの検出2012

    • Author(s)
      金子郁子、宮本和明、三木康弘、湯峯奈都子、西尾真智子、秋本茂
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] ヒトパラインフルエンザウイルス2型(hPIV2)V蛋白によるcaspase-1活性化抑制2012

    • Author(s)
      西尾真智子、太田圭介、湯峰奈都子、五藤秀男
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] Nucleocytoplasmic shuttling of the human parainfluenza virus type 2 P protein2011

    • Author(s)
      Nishio M, Ohtsuka J, Tsurudome M, Nosaka T, Kolakofsky D
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of amino acids of simian virus 41 (SV41) fusion protein that convert parainfluenza virus 5 fusion protein to a protein which specifically interacts with SV41 hemagglutinin-neuraminidase by substitution2011

    • Author(s)
      Tsurudome M, Nakahashi M, Matsushima Y, Nishio M, Kawano M, Nosaka T
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo
    • Related Report
      2013 Final Research Report
  • [Presentation] Antagonistic activity of paramyxovirus V proteins against toll-like receptor 7/9 dependent alpha interferon induction2011

    • Author(s)
      Kitagawa Y, Yamaguchi M, Zhou M, Komatsu T, Nishio M, Sugiyama T, Takeuchi K, Itoh M, Gotoh B
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of amino acids of simian virus 41 (SV41) fusion protein that convert parainfluenza virus 5 fusion protein to a protein which specifically interacts with SV41 hemagglutinin-neuraminidase by substitution.2011

    • Author(s)
      Tsurudome M, Nakahashi M, Matsushima Y, Nishio M, Kawano M, Komada H, Nosaka T.
    • Organizer
      IUMS2011
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report
  • [Presentation] Antagonistic activity of paramyxovirus V proteins against toll-like receptor 7/9 dependent alpha interferon induction.2011

    • Author(s)
      Kitagawa Y, Yamaguchi M, Zhou M, Komatsu T, Nishio M, Sugiyama T, Tadeuchi K, Itoh M, Gotoh B.
    • Organizer
      IUMS2011
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report
  • [Presentation] Nucleocytoplasmic shuttling of human parainfluenza virus type 2 P protein.2011

    • Author(s)
      Nishio M, Ohtsuka j, tsurudome M, Nosaka T, Kolakofsky D.
    • Organizer
      IUMS2011
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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