Analysis of oxidation level of low-density lipoprotein particles

• Project/Area Number: 23590655
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Hokkaido University
• Principal Investigator: TAKEDA SEIJI 北海道大学, 保健科学研究院, 特任准教授 (80374726)
• Co-Investigator(Kenkyū-buntansha): CHIBA Hitoshi 北海道大学, 大学院保健科学研究院, 教授 (70197622) ; SUEOKA Kazuhisa 北海道大学, 情報科学研究科, 教授 (60250479)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: 低比重リポタンパク質 / 原子間力顕微鏡 / 酸化 / 表面電位 / 弾性率 / 硬さ / 一粒子 / リポ蛋白質 / ケルビン測定

Research Abstract

Properties of LDL before and after metal induced oxidation were investigated by an atomic force microscopy system. Surface potential of the LDL decreased with increasing oxidation degree of the LDL. The distribution of the potential before oxidation was wider than that of the potential after oxidation.
Stiffness of the LDL before and after oxidation also decreased with increasing oxidation degree. Distribution of the stiffness of the LDL before oxidation was wide and the stiffness of some LDL particles showed soft such like oxidized LDL. Gel-filtrated sample of oxidized LDL that included the soft LDL induced aggregations of blood platelet and enhance destruction of red blood cells. It is suggested that soft LDL might be related to cardiac disease and that the distribution the stiffness of the LDL might be a novel tool to evaluate not only oxidation degree of the LDL but also to evaluate the degree of the health condition.

Research Products

• [Journal Article] Evaluation of Antioxidant Activity of Natural and Synthetic Compounds Against LDL oxidation Using CNT electrode (2014)
  • Author(s): Futaba Ohkawa, Seiji Takeda, Shu-Ping Hui, Toshihiro Sakurai, Shigeki Jin, Hirotoshi Fuda, Kazuhisa Sueoka, Hitoshi Chiba
  • Journal Title: IEEE SENSORS JOURNAL Volume: vol.14,No12 Issue: 2 Pages: 532-537
  • DOI: 10.1109/jsen.2013.2285408
  • Peer Reviewed
• [Journal Article] Evaluation of Oxidized-Low-Density Lipoproteins Using Kelvin Force Microscopy (2013)
  • Author(s): Seiji Takeda, Futaba Ohkawa, Shu-Ping Hui, Toshihiro Sakurai, Shigeki Jin, Hirotoshi Fuda, Kazuhisa Sueoka, Hitoshi Chiba
  • Journal Title: IEEE SENSORS JOURNAL Volume: vol.13,No9 Issue: 9 Pages: 3449-3453
  • DOI: 10.1109/jsen.2013.2265014
  • Peer Reviewed
• [Journal Article] Application of Kelvin force microscopy for evaluation of oxidized low-density lipoprotein (2012)
  • Author(s): Seiji Takeda, Futaba Ohkawa, Toshihiro Sakurai, Shigeki Jin, Hirotoshi Fuda, Shu-Ping Hui, Kazuhisa Sueoka, Hitoshi Chiba
  • Journal Title: Proceeding of IEEE SENSORS Pages: 1751-1754
• [Journal Article] Application of Kelvin force microscopy for evaluation of oxidized low-density lipoprotein (2012)
  • Author(s): Takeda, Seiji; Ohkawa, Futaba; Sakurai, Toshihiro; Jin, Shigeki; Fuda, Hirotoshi; Hui, Shu-Ping; Sueoka, Kazuhisa Chiba, Hitoshi
  • Journal Title: IEEE SENSORS 2012 Volume: 1 Pages: 1751-1754
  • DOI: 10.1109/icsens.2012.6411519
• [Presentation] 走査型プローブ顕微鏡を利用した一粒子レベルでの低比重リポ蛋白質の酸化状態の評価 (2014)
  • Author(s): 武田晴治, 武笠幸一, 惠淑萍, 布田博敏, 神繁樹, 布田博敏, 末岡和久, 千葉仁志
  • Organizer: 第81回電気化学会
  • Place of Presentation: 大阪
• [Presentation] Investigation of antioxidant activity of compounds against LDL oxidation using a CNT electrode (2014)
  • Author(s): Seiji Takeda, Shu-Ping Hui, Futaba Ohkawa, Shigeki Jin, Hirotoshi Fuda, Koichi Mukasa, Kazuhisa Sueoka, Hitoshi Chiba
  • Organizer: SFRRI2014
  • Place of Presentation: 京都
• [Presentation] Properties of oxidized LDL were investigated by a carbon nanotube based electrode (2014)
  • Author(s): Syun Terashima, Seiji Takeda, Shu-Ping Hui, Futaba Ohkawa, Shigeki Jin, Hirotoshi Fuda, Koichi Mukasa. Kazuhisa Sueoka. Hitoshi Chiba
  • Organizer: SFRRI2014
  • Place of Presentation: 京都
• [Presentation] 走査型プローブ顕微鏡を利用した一粒子レベルでの低比重リポ蛋白質の酸化状態の評価 (2014)
  • Author(s): 武田晴治、武笠幸一、惠淑萍、布田博敏、神繁樹、布田博敏、末岡和久、千葉仁志
  • Organizer: 第81回電気化学会
  • Place of Presentation: 関西大学 (大阪府)
• [Presentation] Investigation of antioxidant activity of compounds against LDL oxidation using a CNT electrode (2014)
  • Author(s): Seiji Takeda; Shu-Ping Hui; Futaba Ohkawa; Shigeki Jin; Hirotoshi Fuda;Koichi Mukasa; Kazuhisa Sueoka; Hitoshi Chiba
  • Organizer: SFRRI 2014
  • Place of Presentation: 京都国際会議場 (京都府)
• [Presentation] Properties of oxidized LDL were investigated by a carbon nanotube based electrode (2014)
  • Author(s): Syun Terashima; Seiji Takeda; Shu-Ping Hui; Futaba Ohkawa; Shigeki Jin; Hirotoshi Fuda;Koichi Mukasa; Kazuhisa Sueoka; Hitoshi Chiba
  • Organizer: SFRRI 2014
  • Place of Presentation: 京都国際会議場 (京都府)
• [Presentation] ケルビン測定を利用した低比重リポ蛋白質の酸化状態の評価 (2013)
  • Author(s): 武田晴治, 惠淑萍, 武笠幸一, 櫻井俊宏, 神繁樹, 布田博敏, 末岡和久, 千葉仁志
  • Organizer: 第80回電気化学会
  • Place of Presentation: 仙台
  • Year and Date: 2013-03-31
• [Presentation] 原子間力顕微鏡によるLDL酸化状態評価 (2013)
  • Author(s): 武田晴治, 大川芙多葉, 櫻井俊宏, 神繁樹, 布田博敏, 惠淑萍, 千葉仁志
  • Organizer: 第53回日本臨床化学会年次学術集会
  • Place of Presentation: 徳島
• [Presentation] 原子間力顕微鏡によるLDL酸化状態評価 (2013)
  • Author(s): 武田晴治，大川芙多葉，櫻井俊宏，神繁樹，布田博敏，惠淑萍，千葉仁志
  • Organizer: 53回日本臨床化学会年次学術集会
  • Place of Presentation: 徳島県郷土文化会館 (徳島県)
• [Presentation] ケルビン測定を利用した低比重リポ蛋白質の酸化状態の評価 (2013)
  • Author(s): 武田晴治、惠淑萍、武笠幸一、櫻井俊宏、神繁樹、布田 博敏、 末岡和久、千葉仁志
  • Organizer: 第80回 電気化学会
  • Place of Presentation: 東北大学(仙台市)
• [Presentation] Application of Kelvin force microscopy for evaluation of oxidized low-density lipoprotein (2012)
  • Author(s): Seiji Takeda, Futaba Ohkawa, Toshihiro Sakurai, Shigeki Jin, Hirotoshi Fuda, Shu-Ping Hui, Kazuhisa Sueoka, Hitoshi Chiba
  • Organizer: IEEE SENSORS 2012
  • Place of Presentation: 台北台湾
  • Year and Date: 2012-10-30
• [Presentation] 酸化LDL由来物質に関する物理化学的研究 (2012)
  • Author(s): 山田理絵, 武田晴治, 大川芙多葉, 櫻井俊宏, 神繁樹, 布田博敏, 惠淑萍, 千葉仁志
  • Organizer: 日本臨床化学会北海道支部例会
  • Place of Presentation: 旭川
  • Year and Date: 2012-10-06
• [Presentation] 原子間力顕微鏡を用いた酸化LDLの電位変化測定 (2012)
  • Author(s): 武田晴治, 大川芙多葉, 櫻井俊宏, 神繁樹, 布田博敏, 惠淑萍, 千葉仁志
  • Organizer: 第52回日本臨床化学会年次学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 2012-09-08
• [Presentation] 原子間力顕微鏡を用いた酸化LDLの電位変化測定 (2012)
  • Author(s): 武田晴治、大川芙多葉、櫻井俊宏、神繁樹、布田博敏、惠淑萍、千葉仁志
  • Organizer: 第５２回日本臨床化学会年会年次学術集会
  • Place of Presentation: いわて県民情報交流センター(盛岡市)
• [Presentation] 酸化LDL由来物質に関する物理化学的研究 (2012)
  • Author(s): 山田理絵，武田晴治，大川芙多葉，櫻井俊宏，神 繁樹，布田博敏，惠 淑萍， 千葉仁志
  • Organizer: 第22回日本臨床化学会 北海道支部例会
  • Place of Presentation: 旭川医大 (旭川市)
• [Presentation] Application of Kelvin force microscopy for evaluation of oxidized low-density lipoprotein (2012)
  • Author(s): Takeda, Seiji; Ohkawa, Futaba; Sakurai, Toshihiro; Jin, Shigeki; Fuda, Hirotoshi; Hui, Shu-Ping; Sueoka, Kazuhisa Chiba, Hitoshi
  • Organizer: IEEE SENSORS 2012
  • Place of Presentation: Taipei International Convention Center (Taiwan)
• [Remarks] 北海道大学高度脂質分析ラボの共用機器にリンクしたページを作成予定