The elucidation of the acquisition of resistance mechanism in multidrug-resistant Pseudomonas aeruginosa and application to simple diagnosis system and infection control
| Project/Area Number |
23590691
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokai University |
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Principal Investigator |
ASAI Satomi 東海大学, 医学部, 講師 (60365989)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYACHI Hayato 東海大学, 医学部, 教授 (20174196)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 緑膿菌 / アシネトバクター / 多剤耐性 / 耐性獲得機構 / 迅速検査 / 感染制御 / 耐性緑膿菌 / 耐性アシネトバクター / 院内感染 / MDRP / Mex pump / 迅速診断検査 |
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| Research Abstract |
The antimicrobial resistance and the expression of the drug resistance related genes were investigated using clinical isolates of drug resistant Pseudomonas aeruginosa for the development of the examination for simple and quick diagnosis system. Using the clinical isolated P. aeruginosa which had co-expression of MexEF-OprN and MexXY-OprM, the development of the diagnosis systems for the detection of the resistance gene by observing presence of each gene expression visually and Enzyme-linked immunosorbent assay (the ELISA method) were tried.
The contribution of the drug effluxing pump was not strong in Acinetobacter baumannii, and contribution of other gene expression was considered.
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Report
(4 results)
Research Products
(91 results)
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[Journal Article] 造血幹細胞移植患者における内因性細菌叢監視培養検査の臨床的意義2013
Author(s)
大島利夫, 浅井さとみ, 大菅淳, 山本由香梨, 野宮沙織, 矢部みはる, 矢部普正, 加藤俊一, 鬼塚真仁, 安藤潔, 大崎敬子, 神谷茂, 宮地勇人
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Journal Title
J Germfree Life Gnotobiol
Volume: 43
Pages: 91-96
Related Report
Peer Reviewed
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[Journal Article] Localized or diffuse lesions of the submandibular glands in IgG4-related disease in association with differential organ involvement.2013
Author(s)
Asai, S, Okami K, Shiraishi, S, Sugimoto R, Damdinsuren A, Sato S, Matsushita H, Suzuki Y, Miyachi H.
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Journal Title
J Ultrasound in Med
Volume: 32
Issue: 5
Pages: 731-736
DOI
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Peer Reviewed
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[Journal Article] Clinical and pathological features of B-cell non-Hodgkin lymphomas lacking the surface expression of immunoglobulin light chains.2012
Author(s)
Matsushita H, Nakamura N, Tanaka Y, Ohgiya D, Tanaka Y, Damdinsuren A, Asai S, Yabe M, Kawada H, Ogawa Y, Ando K, Miyachi H.
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Journal Title
Clin Chem Lab Med
Volume: 50
Pages: 1665-1670
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Peer Reviewed
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[Book] 尿沈渣アトラス2012
Author(s)
野崎司, 浅井さとみ, 宮地勇人
Total Pages
172
Publisher
Sysmex
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