A basic study for evaluation method of brain function on forensic autopsy cases.

• Project/Area Number: 23590851
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: The University of Tokushima
• Principal Investigator: NISHIMURA Akiyoshi 徳島大学, ヘルスバイオサイエンス研究部, 教授 (60283561)
• Co-Investigator(Kenkyū-buntansha): TOKUNAGA Itsuo 徳島大学, 大学院ヘルスバイオサイエンス研究部, 准教授 (30116842) ; ISHIGAMI Akiko 徳島大学, 大学院ヘルスバイオサイエンス研究部, 助教 (60359916) ; NUSHIDA Hideyuki 兵庫医科大学, 医学部, 講師 (90335448)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 法医病理学 / dementia / schizophrenia / hippocampus / lectin / erythropoietin receptor / NOS / N-Cadherin / chondroitin sulfate / Alzheimer / Braak stage / dopaminergic / serotonin / cholinergic / microglia / astrocyte / レクチン / アルツハイマー / Gallias-Braak / 海馬歯状回

Research Abstract

Autopsied brains with dementia were examined by means of immunohistochemical staining method using 17 antibodies, related with neuronal degeneration, cellular damage and oxidative stress. Especially, a large number of SPDs were stained by antibodies of YKL-39, Tau, CS and HSP70. Furthermore, development frequency of SPDs between by mean of NOS and GFAP, NOS and CS, ApoE and CS, Tau and HSP70, and CS and HSP70, showed positive correlations. Substances relating with dementia are detected in same SPD by mean of multi fluorescent immunehistochemical staining visualized by confocal laser microscopy. Although, SPD were related with apoptosis of neurons in schizophrenia, SPD might come from not only neuronal degeneration but also glial in degenerative dementia. SPDs with reactivity of EAAC1 and/or alpha-syn related with neuronal degeneration were detected in the early stages and they with reactivity of S-100 and/or YKL-40 related with astrocytes were detected in the middle or later stages.

Research Products

• [Presentation] Histopathological investigations of lectin positive spherical deposits detected in the molecular layer of the hippocampal formation with neurofibrillary tangles (2014)
  • Author(s): Akiyoshi NISHIMURA, Akiko ISHIGAMI,Hiromitsu, KURATA, Itsuo TOKUNAGA
  • Organizer: 9th International Symposium on Advances in Legal Medicine (ISALM)
  • Place of Presentation: Fukuoka International Congress Center (Fukuoka Prefecture, Fukuoka City)
• [Presentation] Histopathological investigations of lectin positive spherical deposits detected in the molecular layer of the hippocampal formation with neurofibrillary tangles. (2014)
  • Author(s): Akiyoshi NISHIMURA, Akiko ISHIGAMI，Hiromitsu, KURATA, Itsuo TOKUNAGA
  • Organizer: The 9th International ADVANCES IN LEGAL MEDICINE
  • Place of Presentation: Fukuoka International Congress Center(福岡県)
• [Presentation] ヒト剖検脳の海馬歯状回分子層における球状沈着物の免疫組織化学的検討 (2013)
  • Author(s): 山田茉樹, 石上安希子, 徳永逸夫, 西村明儒
  • Organizer: 日本法医学会総会
  • Place of Presentation: ロイトン札幌(北海道札幌市)