Enhancement of natural immunity in influenza by maoto
| Project/Area Number |
23590906
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General internal medicine (including Psychosomatic medicine)
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | インフルエンザ / 漢方 / 麻黄湯 / 抗インフルエンザ薬 / PR8 / オートファジー / エンドサイトーシス / 漢方薬 |
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| Outline of Final Research Achievements |
Although maoto has been known to be effective traditionally in influenza, little is known about its mechanisms. We investigated the anti-virus capacity of maoto in in-vitro system using A549 cell line infected with influenza virus (PR8). Twenty-four hours later, virus titer in medium, viral RNA, and viral proteins were significantly reduced when added with maoto. To investigate the phase of the experimental infection at which maoto is effective, we added it to culture wells at different times and assayed the virus titer and intracellular virus RNA level 24 hr post-infection. When maoto was added in the pre-treatment, infection, and early propagation phases (<8 hrs), the virus titers and viral RNA were significantly lower than with medium only.
We suggest that maoto may block the invasion of influenza virus to cytoplasm or inhibit the early propagation phase of influenza virus.
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Report
(5 results)
Research Products
(9 results)
