The analysis of the shift from gastric to intestinal phenotype of stem cell in the stomach mucosa

• Project/Area Number: 23590921
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Nagoya City University
• Principal Investigator: MIZOSHITA Tsutomu 名古屋市立大学, 医学(系)研究科(研究院), 助教 (40347414)
• Co-Investigator(Kenkyū-buntansha): TANIDA Satoshi 名古屋市立大学, 大学院医学研究科, 講師 (30528782) ; JOH Takashi 名古屋市立大学, 大学院医学研究科, 教授 (30231369)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 胃幹細胞 / 三次元培養系

Research Abstract

We established a long-term three-dimensional primary culture of mouse glandular stomach epithelial cells, being cultivated for more than 2 months and maintaining the gastric phenotype without intestinal one. We consider that the shift from gastric to intestinal phenotype of stem cell in the stomach mucosa may be clarified by applying the above-mentioned three-dimensional primary culture to the glandular stomach of Helicobacter pylori-infected Mongolian gerbil model in which the intestinal metaplasia often occurs.

Research Products

• [Journal Article] Adalimumab Treatment in Biologically Naïve Crohn's Disease : Relationship with Ectopic MUC5AC Expression and Endoscopic Improvement (2014)
  • Author(s): Mizoshita, T., Tanida, S., Tsukamoto, H., Ozeki, K., Katano, T., Nishiwaki, H., Ebi, M., Mori, Y., Kubota, E., Kataoka, H., Kamiya, T., Joh, T
  • Journal Title: Gastroenterol Res Pract Volume: 2014 Pages: 687257-687257
  • DOI: 10.1155/2014/687257
  • Peer Reviewed
• [Journal Article] Colon Mucosa Exhibits Loss of Ectopic MUC5AC Expression in Patients with Ulcerative Colitis Treated with Oral Tacrolimus. (2013)
  • Author(s): Mizoshita, T., Tanida, S., Tsukamoto, H., Ozeki, K., Katano, T., Ebi, M., Mori, Y., Kataoka, H., Kamiya, T., and Joh, T.
  • Journal Title: ISRN Gastroenterol Volume: 2013 Pages: 304894-304894
  • DOI: 10.1155/2013/304894
  • Peer Reviewed
• [Journal Article] Maintenance of the remission stage of Crohn's disease with adalimumab therapy during pregnancy (2013)
  • Author(s): Mizoshita, T., Tanida, S., Tsukamoto, H., Ozeki, K., Katano, T., Ebi, M., Mori, Y., Kataoka, H., Kamiya, T., and Joh, T
  • Journal Title: Intern Med Volume: 52 Pages: 1049-53
  • NAID: 130003365608
  • URL: https://www.jstage.jst.go.jp/article/internalmedicine/52/10/52_52.9466/_article
  • Peer Reviewed
• [Journal Article] Establishment of a long-term three-dimensional primary culture of mouse glandular stomach epithelial cells within the stem cell niche. (2013)
  • Author(s): Katano, T., Ootani, A., Mizoshita, T., Tanida, S., Tsukamoto, H., Ozeki, K., Ebi, M., Mori, Y., Kataoka, H., Kamiya, T., Toda, S., Joh, T.
  • Journal Title: Biochem Biophys Res Commun. Volume: 432 Issue: 4 Pages: 558-563
  • DOI: 10.1016/j.bbrc.2013.02.051
  • Peer Reviewed
• [Journal Article] Maintenance of the remission stage of Crohn's disease with adalimumab therapy during pregnancy. (2013)
  • Author(s): Mizoshita, T., Tanida, S., Tsukamoto, H., Ozeki, K., Katano, T., Ebi, M., Mori, Y., Kataoka, H., Kamiya, T., and Joh, T.
  • Journal Title: Intern Med Volume: 52 Pages: 1049-53
  • NAID: 130003365608
  • Peer Reviewed
• [Journal Article] Adalimumab therapy following granulocyte and monocyte adsorptive apheresis in a patient with Crohn's disease accompanied by chronic myeloid leukemia (2012)
  • Author(s): Mizoshita, T., Tanida, S., Kusumoto, S., Ozeki, K., Tsukamoto, H., Ebi, M., Mori, Y., Kataoka, H., Kamiya, T., Joh, T
  • Journal Title: Clin J Gastroenterol Volume: 5 Pages: 302-306
  • URL: http://link.springer.com/article/10.1007%2Fs12328-012-0314-5
  • Peer Reviewed
• [Journal Article] Adalimumab therapy following granulocyte and monocyte adsorptive apheresis in a patient with Crohn's disease accompanied by chronic myeloid leukemia. (2012)
  • Author(s): Mizoshita, T., Tanida, S., Kusumoto, S., Ozeki, K., Tsukamoto, H., Ebi, M., Mori, Y., Kataoka, H., Kamiya, T., Joh, T.
  • Journal Title: Clin J Gastroenterol. Volume: 5 Pages: 302-306
  • Peer Reviewed
• [Journal Article] Lansoprazole induces collagenous colitis in the colon of Mongolian gerbils (2011)
  • Author(s): Mizushima, T., Mizoshita, T., Sasaki, M., Tanida, S., Tsukamoto, H., Shimura, T., Kanematsu, T., Kataoka, H., Kamiya, T., Tsukamoto, T., Tatematsu, M., Joh, T
  • Journal Title: Asian Pac J Cancer Prev Volume: 12 Pages: 2759-62
  • URL: http://www.apocpcontrol.org/page/apjcp_issues_view.php?sid=Entrez:PubMed&id=pmid:22320988&key=2011.12.10.2759
  • Peer Reviewed
• [Journal Article] Long-term high-dose proton pump inhibitor administration to Helicobacter pylori-infected Mongolian gerbils enhances neuroendocrine tumor development in the glandular stomach (2011)
  • Author(s): Tsukamoto, H., Mizoshita, T., Sasaki, M., Mizushima, T., Tanida, S., Ozeki, K., Hirata, Y., Shimura, T., Kataoka, H., Kamiya, T., Nojiri, S., Tsukamoto, T., Tatematsu, M., Joh, T
  • Journal Title: Asian Pac J Cancer Prev Volume: 12 Pages: 1049-54
  • URL: http://www.apocpcontrol.org/page/apjcp_issues_view.php?sid=Entrez:PubMed&id=pmid:21790250&key=2011.12.4.1049
  • Peer Reviewed
• [Journal Article] Long-term High-dose Proton Pump Inhibitor Administration to Helicobacter pylori-infected Mongolian gerbils Enhances Neuroendocrine tumor Development in the Glandular Stomach. (2011)
  • Author(s): Tsukamoto H, Mizoshita T, Sasaki M, Mizushima T, Tanida S, Ozeki K, Hirata Y, Shimura T, Kataoka H, Kamiya T, Nojiri S, Tsukamoto T, Tatematsu M, Joh T.
  • Journal Title: Asian Pac J Cancer Prev. Volume: 12(4) Pages: 1049-54
  • Peer Reviewed
• [Journal Article] Lansoprazole induces collagenous colitis in the colon of Mongolian gerbils. (2011)
  • Author(s): Mizushima T, Mizoshita T, Sasaki M, Tanida S, Tsukamoto H, Shimura T, Kanematsu T, Kataoka H, Kamiya T, Tsukamoto T, Tatematsu M, Joh T.
  • Journal Title: Asian Pac J Cancer Prev. Volume: 12(10) Pages: 2759-62
  • Peer Reviewed
• [Presentation] Adalimumabを投与したbiologic naive Crohn's disease症例の臨床的検討 (2014)
  • Author(s): 溝下勤, 谷田諭史, 尾関啓司, 塚本宏延, 片野敬仁, 林則之, 田中守, 濱野真吾, 西脇裕高, 海老正秀, 澤田武, 森義徳, 久保田英嗣, 片岡洋望, 城卓志
  • Organizer: 第10回日本消化管学会総会学術集会, 口演(一般演題, オーラルセッション9)
  • Place of Presentation: 福島(コラッセふくしま)
  • Year and Date: 2014-02-14
• [Presentation] Long-term three-dimensional primary gastric culture under an air-liquid interface environment (2013)
  • Author(s): Katano, T., Mizoshita, T., Joh, T
  • Organizer: 21th United European Gastroenterology Week
  • Place of Presentation: Germany(Berlin)
  • Year and Date: 2013-10-14
• [Presentation] ムチンコア蛋白MUC5ACは潰瘍性大腸炎の疾患活動性マーカーとして有用である-Tacrolimusを導入した中等症・重症例での検討- (2013)
  • Author(s): 溝下勤, 谷田諭史, 尾関啓司, 塚本宏延, 片野敬仁, 林則之, 田中守, 濱野真吾, 西脇裕高, 海老正秀, 森義徳, 久保田英嗣, 片岡洋望, 城卓志
  • Organizer: 第55回日本消化器病学会大会(第21回日本消化器関連学会週間)
  • Place of Presentation: 東京(品川プリンスホテル)(示説 大腸(潰瘍性大腸炎1))
  • Year and Date: 2013-10-10
• [Presentation] 当院でのbiologic naive Crohn's diseaseに対するadalimumab治療 (2013)
  • Author(s): 溝下勤, 谷田諭史, 尾関啓司, 塚本宏延, 城卓志
  • Organizer: 第9回日本消化管学会総会学術集会, IBD臨床4一般演題オーラルセッション27
  • Place of Presentation: 東京(京王プラザホテル)(O27-4)
  • Year and Date: 2013-01-26
• [Presentation] Tacrolimus・顆粒球単球吸着(GMA)併用療法による中等症・重症潰瘍性大腸炎症例への寛解導入の試み–当院での検討- (2012)
  • Author(s): 溝下勤, 谷田諭史, 尾関啓司, 塚本宏延, 城卓志
  • Organizer: 第54回日本消化器病学会大会(第20回日本消化器関連学会週間)
  • Place of Presentation: 神戸(神戸国際展示場)(示説{大腸(潰瘍性大腸炎)6})
  • Year and Date: 2012-10-11
• [Presentation] スナネズミはlansoprazole-associated collagenous colitis解析モデルとして有用である (2012)
  • Author(s): 溝下勤, 塚本宏延, 谷田諭史, 尾関啓司, 城卓志
  • Organizer: 第49回日本消化器免疫学会総会
  • Place of Presentation: 鹿児島(城山観光ホテル)(一般口演(一般演題, 消化管1))
  • Year and Date: 2012-07-05
• [Presentation] Biologic naïve Crohn's diseaseに対するadalimumab治療-名古屋市立大学病院での検討- (2012)
  • Author(s): 溝下勤, 谷田諭史, 塚本宏延, 尾関啓司, 水島隆史, 城卓志
  • Organizer: 第8回日本消化管学会総会学術集会
  • Place of Presentation: 仙台(仙台国際センター)(口演(ポスター, ポスターセッション22症例報告13, P-22-3))
  • Year and Date: 2012-02-10
• [Presentation] ランソプラゾール持続投与が胃腫瘍発生に与える影響–スナネズミモデルでの検討 (2011)
  • Author(s): 溝下勤, 佐々木誠人, 谷田諭史, 志村貴也, 森義徳, 片岡洋望, 神谷武, 城卓志
  • Organizer: 第22回日本消化器癌発生学会総会
  • Place of Presentation: 佐賀(ホテルニューオータニ佐賀)(一般口演)
  • Year and Date: 2011-11-25
• [Presentation] 高齢者の潰瘍性大腸炎患者の治療-名古屋市立大学病院の現状-(ワークショップ13高齢者における消化器疾患の診断と治療) (2011)
  • Author(s): 溝下勤, 谷田諭史, 城卓志
  • Organizer: 第53回日本消化器病学会大会(第19回日本消化器関連学会週間), ワークショップ
  • Place of Presentation: 福岡(福岡サンパレスパレスルーム)
  • Year and Date: 2011-10-22
• [Presentation] ランソプラゾール持続投与が胃腫瘍発生に与える影響 -スナネズミモデルでの検討 (2011)
  • Author(s): 溝下 勤 、佐々木誠人、谷田諭史、志村貴也、森 義徳、片岡洋望、神谷 武、城 卓志
  • Organizer: 第22回日本消化器癌発生学会総会
  • Place of Presentation: 佐賀（ホテルニューオータニ佐賀）
• [Presentation] ムチンコア蛋白MUC5ACは潰瘍性大腸炎の疾患活動性マーカーとして有用である -Tacrolimusを導入した中等症・重症例での検討-
  • Author(s): 溝下 勤、谷田諭史、尾関啓司、塚本宏延、片野敬仁、林 則之、田中 守、濱野真吾、西脇裕高、海老 正秀、森 義徳、久保田英嗣、片岡洋望、城 卓志
  • Organizer: 第55回 日本消化器病学会大会（第21回 日本消化器関連学会週間）
  • Place of Presentation: 東京（品川プリンスホテル）
• [Presentation] Adalimumabを投与したbiologic naive Crohn's disease症例の臨床的検討
  • Author(s): 溝下 勤、谷田諭史、尾関啓司、塚本宏延、片野敬仁、林 則之、田中 守、濱野真吾、西脇裕高、海老正秀、澤田 武、森 義徳、久保田英嗣、片岡洋望、城 卓志
  • Organizer: 第10回日本消化管学会総会学術集会
  • Place of Presentation: 福島（コラッセふくしま）
• [Presentation] Long-term three-dimensional primary gastric culture under an air-liquid interface environment.
  • Author(s): Takahito Katano、Tsutomu Mizoshita、Takashi Joh
  • Organizer: 21th United European Gastroenterology Week
  • Place of Presentation: Berlin (Germany)
• [Presentation] スナネズミはlansoprazole-associated collagenous colitis解析モデルとして有用である
  • Author(s): 溝下 勤、塚本宏延、谷田諭史、尾関啓司、城 卓志
  • Organizer: 日本消化器免疫学会総会
  • Place of Presentation: 城山観光ホテル（鹿児島）