Ready to go for treatment of Inflammatory bowel disease by using analyzing MRP4 SNPs.

• Project/Area Number: 23590936
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: BAN Hiromitsu 滋賀医科大学, 医学部, 特任助教 (30598363)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 炎症性腸疾患 / 潰瘍性大腸炎 / 薬剤感受性遺伝子 / 遺伝子一塩基多型 / MRP4 / クローン病 / ITPase / TPMT

Research Abstract

Sample DNA of ulcerative colitis, the Crohn disease patient who collected it by 2014 and the physically unimpaired person volunteer examined 279 personality in total, SNP of 3 genes in the both sides of the TaqMan probe method and the conventional Direct sequence method. As for the MRP4 G2269A SNP, 68 people, A/A were seven people G/A, and the allyl frequency was 14.7%. As for the TPMT A719G SNP, five people, G/G were 0 people A/G, and the allyl frequency was 0.9%. As for the ITPA C94A SNP, 61 people, A/A were six people C/A, and the allyl frequency was 13.1%. There was no great difference and a result of HapMap, and there was a difference by the TaqMan probe method and the Direct sequence method neither. It was revealed that the TaqMan probe method using TaqMan SNP Genotyping Assays was more useful for quick SNP analysis than the above-mentioned result.

Research Products

• [Journal Article] Polymorphisms of the Niemann-Pick C1-like 1 gene in a Japanese population. (2013)
  • Author(s): Osaki R, Imaeda H, Takahashi K, Fujimoto T, Takeuchi T, Fujiyama Y, Andoh A.
  • Journal Title: Biomed Rep. Volume: 1 Issue: 1 Pages: 156-160
  • DOI: 10.3892/br.2012.24
  • Peer Reviewed
• [Journal Article] 炎症性腸疾患-病因解明と診断・治療の最新知見-特論チオプリン代謝関連酵素の遺伝子多型と日本人における特徴 (2012)
  • Author(s): 安藤朗, 今枝広丞, 大崎理恵, 伴宏充
  • Journal Title: 日本臨床 Volume: 70(1) Pages: 639-645
  • Peer Reviewed
• [Journal Article] Accuracy of genotyping using the TaqMan PCR assay for single nucleotide polymorphisms responsible for thiopurine sensitivity in Japanese patients with inflammatory bowel disease (2011)
  • Author(s): Osaki R, Imaeda H, Ban H, Aomatsu T, Bamba S, Tsujikawa T, Sasaki M, Fujiyama Y, Andoh A
  • Journal Title: Exp Ther Med Volume: 2(5) Pages: 783-786
  • Peer Reviewed
• [Journal Article] 潰瘍性大腸炎のTherapeutic Strategy Standardな治療法から最新情報まで-病態解析に基づく実現性の高い治療戦略-日本人特有のチオプリン高感受性遺伝子の同定とテーラーメイド医療の展開 (2011)
  • Author(s): 安藤朗, 伴宏充, 大崎理恵, 今枝広丞
  • Journal Title: Intestine Volume: 15(3) Pages: 267-272
• [Presentation] 遺伝子多型解析と消化器疾患炎症性腸疾患患者におけるチオプリン高感受性遺伝子の検討 (2013)
  • Author(s): 伴宏充, 安藤朗, 藤山佳秀
  • Organizer: 第99回日本消化器病学会総会
• [Presentation] 遺伝子多型解析と消化器疾患 炎症性腸疾患患者におけるチオプリン高感受性遺伝子の検討 (2013)
  • Author(s): 伴 宏充, 安藤 朗, 藤山 佳秀
  • Organizer: 日本消化器病学会
  • Place of Presentation: 東京
• [Presentation] 日本人におけるNiemann-Pick C1-like 1遺伝子多型の解析 (2013)
  • Author(s): 大崎 理英, 今枝 広丞, 辻川 知之, 佐々木 雅也, 斎藤 康晴, 藤山 佳秀, 安藤 朗
  • Organizer: 日本内科学会
  • Place of Presentation: 東京
• [Presentation] MRP4/ABCC4遺伝子多型と炎症性腸疾患日本人特有のチオプリン高感受性との関連 (2011)
  • Author(s): 安藤朗, 今枝広丞, 伴宏充
  • Organizer: 第84回日本生化学会大会
• [Presentation] アザチオプリン/6-メルカプトプリン投与時におけるTaqMan probeを用いたチオプリン体代謝酵素一塩基多型の迅速診断法 (2011)
  • Author(s): 大崎理英, 安藤朗, 伴宏充,今枝広丞, 馬場重樹, 塩谷淳, 青松友槻, 稲富理, 佐々木雅也, 辻川知之, 斎藤康晴, 藤山佳秀
  • Organizer: 第53回日本消化器病学会大会