Artifical kidney established by human kidney derived iPS cells

• Project/Area Number: 23591183
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: The University of Tokyo
• Principal Investigator: HISHIKAWA Keiichi 東京大学, 医学部附属病院, 准教授 (50255460)
• Co-Investigator(Renkei-kenkyūsha): FUJITA Toshiro 東京大学, 先端研, 特任教授 (10114125) ; NAKAUCHI Hiromitsu 東京大学, 医科学研究所, 教授 (40175485) ; ABURATANI Hiroyuki 東京大学, 先端研, 教授 (10202657)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 幹細胞 / iPS cells / メチル化 / 再生医学 / iPS細胞 / 再生医療 / マイクロアレイ

Outline of Final Research Achievements

In this study, we firstly identified key genes that determine memory of kidney derived iPS cells. By using these genes, we induced kidney cells efficiently from iPS cells. Finally, we tried to establish artificial kidney that was reported by Lanza et al. However, we could not reproduce the device by using human kidney derived iPS cells.

Research Products

• [Journal Article] Adult stem-like cells in kidney. (2015)
  • Author(s): Hishikawa K, Takase O, Yoshikawa M, Tsujimura T, Nangaku M, Takato T.
  • Journal Title: World J Stem Cells. Volume: 7 Issue: 2 Pages: 490494-490494
  • DOI: 10.4252/wjsc.v7.i2.490
  • Peer Reviewed / Open Access
• [Journal Article] Immunomodulation with eicosapentaenoic acid supports the treatment of autoimmune small-vessel vasculitis. (2014)
  • Author(s): Hirahashi J, Kawahata K, Arita M, Iwamoto R, Hishikawa K, Honda M, Hamasaki Y, Tanaka M, Okubo K, Kurosawa M, Takase O, Nakakuki M, Saiga K, Suzuki K, Kawachi S, Tojo A, Seki G, Marumo T, Hayashi M, Fujita T.
  • Journal Title: Sci. Rep Volume: 4 Issue: 1 Pages: 6406-6406
  • DOI: 10.1038/srep06406
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Basichelix-loop-helix transcriptional factor MyoR regulates BMP-7 in acute kidney injury. (2013)
  • Author(s): Kamiura N, Hirahashi J, Matsuzaki Y, Idei M, Takase O, Fujita T, TakatoT, Hishikawa K.
  • Journal Title: Am J Physiol RenalPhysiol. Volume: 304(9) Issue: 9 Pages: 2493-2500
  • DOI: 10.1152/ajprenal.00510.2012
  • Peer Reviewed
• [Journal Article] The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells. (2013)
  • Author(s): Takase O, Yoshikawa M, Idei M, Hirahashi J, Fujita T, Takato T, Isagawa T, Nagae G, Suemori H, Aburatani H, Hishikawa K.
  • Journal Title: PLoS One. Volume: 8
  • Peer Reviewed
• [Journal Article] 人工ゲルによる幹細胞機能制御 (2012)
  • Author(s): 菱川慶一
  • Journal Title: 先端バイオマテリアルハンドブック Volume: 1 Pages: 304-308
  • Peer Reviewed
• [Journal Article] Eicosapentaenoic acid regulates IkBa and prevents tubulointestitial injury in kidney (2011)
  • Author(s): Takase O, Hishikawa K, Kamiura M, Kawano H, Mizuguchi K, Fujita T
  • Journal Title: Eur J Pharmacol Volume: 669 Pages: 128-135
  • Peer Reviewed
• [Presentation] 腎臓由来iPS細胞を用いた2つの腎系統特異的分化誘導法の検討 (2015)
  • Author(s): 高瀬 敦、辻村 太郎、出射真奈、宮本寛治、吉川真弘、南学正臣、高戸毅、菱川慶一
  • Organizer: 第１４回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] 腎系統特異的分化刺激を用いた腎上皮細胞由来iPS細胞の検討 (2014)
  • Author(s): 高瀬 敦、吉川真弘、高戸毅、南学正臣、菱川慶一
  • Organizer: 第５７回日本腎臓学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-07-04 – 2014-07-06
• [Presentation] 腎上皮細胞由来iPS細胞と線維芽細胞由来iPS細胞の比較による腎系統特異的分化誘導法の検討 (2014)
  • Author(s): 高瀬 敦、出射 真奈、宮本 寛治、吉川 真弘、高戸 毅、菱川 慶一
  • Organizer: 日本再生医療学会総会
  • Place of Presentation: 京都国際会館
• [Presentation] ヒト腎癌細胞のiPS化における細胞記憶に着目した新規治療標的分子の探索 (2014)
  • Author(s): 菱川 慶一、高瀬 敦、出射 真奈、宮本 寛治、吉川 真弘、高戸 毅
  • Organizer: 日本再生医療学会総会
  • Place of Presentation: 京都国際会館
• [Presentation] 3種類の腎臓由来iPS細胞からの腎臓再生の可能性について (2013)
  • Author(s): 高瀬 敦、吉川 真弘、高戸 毅、菱川 慶一
  • Organizer: 日本腎臓学会学術総会
  • Place of Presentation: 東京フォーラム
• [Presentation] TolvaptanのNF-kappa B を介した抗炎症、腎保護作用の検討 (2013)
  • Author(s): 高瀬 敦、吉川 真弘、高戸 毅、菱川 慶一
  • Organizer: 日本高血圧学会総会
  • Place of Presentation: 大阪国際会議場
• [Presentation] ヒト腎臓由来iPS細胞のDNAメチル化プロファイルと細胞記憶 (2011)
  • Author(s): 菱川慶一、高瀬敦、出射真奈、藤田敏郎
  • Organizer: 日本腎臓学会
  • Place of Presentation: 横浜
• [Book] 再生医療叢書 (2011)
  • Author(s): 菱川慶一
  • Total Pages: 90
  • Publisher: 腎臓再生を目指した包括的戦略