| Project/Area Number |
23591239
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Gifu University |
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Principal Investigator |
KIMURA Akio 岐阜大学, 医学(系)研究科(研究院), 准教授 (00362161)
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|---|
| Co-Investigator(Kenkyū-buntansha) |
YANO Takeo 三重大学, 地域イノベーション学研究科, 教授 (00569197)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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|---|
| Keywords | 抗神経抗体 / 自己免疫介在性脳炎 / 傍腫瘍性神経症候群 / 抗血管内皮抗体 / プロテオーム解析 / 二次元免疫ブロット / 免疫沈降 / 質量分析 |
|---|
| Research Abstract |
We identified novel anti-neural and anti-endothelial cell antibodies in serum and cerebrospinal fluid samples from patients with autoimmune-mediated encephalitis, who had no previous reported anti-neural and anti-endothelial cell antibodies. To identify these novel antibodies, we employed two-dimensional immunoblotting or immunoprecepitation using human neuroblastoma cells, human brain microvascular endothelial cells, or rat brain homogenate as the antigen source. As a result, we identified antibodies against intracellular adhesion molecule 5 in serum from a patient with Hashimoto encephalitis, ryanodine receptor 1 in serum from a patient with paraneoplastic cerebellar degeneration, and myosin regulatory light chain 12A in cerebrospinal fluid from a patient with paraneoplastic limbic encephalitis.
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