Development of the new Helicobacter pylori eradication method for using a phage lytic enzyme
| Project/Area Number |
23591478
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Hiroaki 高知大学, 教育研究部医療学系, 講師 (90346560)
UCHIYAMA Jumpei 高知大学, 教育研究部医療学系, 助教 (20574619)
DAIBATA Masanori 高知大学, 教育研究部医療学系, 教授 (50263976)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | バクテリオファージ / ピロリ菌 / ファージ療法 / 吸着タンパク質 / 溶菌酵素 / 外膜 / リガンド分子 |
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| Research Abstract |
To develop a novel eradication method of Helicobater pylori using bacteriolytic activity of a bacteriophage (phage) KHP30, the morphological, physical, and genetic aspects and the life-cycle of KHP30 have been studied. The host-range of KHP30 was about 65%, suggesting that it is suitable for application use as antibacterial agent. The complete DNA nucleotide sequence was determined and the open reading frames (ORFs) were annotated. The genome consisted of 26 kbp including 30 ORFs. By homology search of the genome, the ORF for the holin protein, one component of general lytic system, was found, but the ORF for the lysin, the counterpart of the system was not found. Therefore, H. pylori phage KHP30 was supposed to have a unique bacteriolytic system different from those of other phages.
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Report
(4 results)
Research Products
(101 results)
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[Journal Article] Genome Analysis of Pseudomonas aeruginosa Bacteriophage KPP23, Belonging to the Family Siphoviridae2014
Author(s)
Yamaguchi K, Miyata R, Shigehisa R,Uchiyama J, Takemura-Uchiyama I, Kato S, Ujihara T, Sakaguchi Y, Daibata M, Matsuzaki S
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Journal Title
Genome Announc
Volume: 2(3)
Related Report
Peer Reviewed
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[Journal Article] Characterization of a novel Pseudomonas aeruginosa bacteriophage, KPP25, of the family Podoviridae2014
Author(s)
Miyata R, Yamaguchi K, Uchiyama J, Shigehisa R, Takemura-Uchiyama I, Kato SI, Ujihara T, Sakaguchi Y, Daibata M, Matsuzaki S
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Journal Title
Virus Res
Volume: 5(189C)
Pages: 43-46
Related Report
Peer Reviewed
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[Journal Article] In silico analysis of AHJD-like viruses, Staphylococcus aureus phages S24-1 and S13', and study of phage S24-1 adsorption2014
Author(s)
Uchiyama J, Takemura-Uchiyama I, Kato S, Sato M, Ujihara T, Matsui H, Hanaki H, Daibata M, Matsuzaki S
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Journal Title
Microbiologyopen
Volume: 3(2)
Pages: 257-70
Related Report
Peer Reviewed
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[Journal Article] Characterization of a novel Pseudomonas aeruginosa bacteriophage, KPP25, of the family Podoviridae.2014
Author(s)
Miyata R, Yamaguchi K, Uchiyama J, Shigehisa R, Takemura-Uchiyama I, Kato SI, Ujihara T, Sakaguchi Y, Daibata M, Matsuzaki S.
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Journal Title
Virus Res.
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Draft Genome Sequence of Clostridium botulinum Type B Strain Osaka05, Isolated from an Infant Patient with Botulism in Japan.2014
Author(s)
Sakaguchi Y, Hosomi K, Uchiyama J, Ogura Y, Umeda K, Sakaguchi M, Kohda T, Mukamoto M, Misawa N, Matsuzaki S, Hayashi T, Kozaki S.
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Journal Title
Genome Announc.
Volume: 2
Related Report
Peer Reviewed
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[Journal Article] In silico analysis of AHJD-like viruses, Staphylococcus aureus phages S24-1 and S13', and study of phage S24-1 adsorption.2014
Author(s)
Uchiyama J, Takemura-Uchiyama I, Kato S, Sato M, Ujihara T, Matsui H, Hanaki H, Daibata M, Matsuzaki S.
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Journal Title
Microbiologyopen.
Volume: 3
Issue: 2
Pages: 257-270
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Pseudomonas aeruginosa keratitis in mice: effects of topical bacteriophage KPP12 administration2012
Author(s)
Fukuda K, Ishida W, Uchiyama J, Rashel M, Kato S, Morita T, Muraoka A, Sumi T, Matsuzaki S, Daibata M, Fukushima A
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Pseudomonas aeruginosa keratitis in mice: effects of topical bacteriophage KPP12 administration.2012
Author(s)
Fukuda K, Ishida W, Uchiyama J, Rashel M, Kato S, Morita T, Muraoka A, Sumi T, Matsuzaki S, Daibata M, Fukushima A
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Journal Title
Related Report
Peer Reviewed
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[Presentation] A Novel type of spherical bacteriophge, Helicobacter pylori bacteriophage KHP30.2013
Author(s)
Uchiyama J, Takeuchi H, Sakagucvhi Y, Gamo K, Kato S, Takemura-Uchiyama I, Ujihara T, Daibata M, Matsuzaki S.
Organizer
5Th Congress of Europea Microbiologists (FEMS 2013)
Place of Presentation
Leipzig, Germany
Related Report
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[Presentation] C型ボツリヌス毒素変換ファージのゲノム情報を基盤とした偽溶原性メカニズムの解析2012
Author(s)
阪口義彦,Oliva Maria A., Martin-Galiano Antonio J., Andreu Jose M., 阪口政清,内山淳平,小椋義俊,山本由弥子,鈴木智典,織田華絵,津田千秋,松崎茂展,林哲也,小熊恵二
Organizer
第35回日本分子生物学会年会
Place of Presentation
福岡国際会議揚・マリンメッセ福岡(福岡)
Related Report
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