A search for candidate genes involved in invasion and metastasis of cutaneous squamous cell carcinoma
| Project/Area Number |
23591624
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
TAKEI Kimiko 琉球大学, 医学(系)研究科(研究院), 助教 (90325861)
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| Co-Investigator(Kenkyū-buntansha) |
UEZATO Hiroshi 琉球大学, 大学院医学研究科, 教授 (10137721)
INAMINE Morihiko 琉球大学, 大学院医学研究科, 助教 (90437989)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 有棘細胞癌 / 転移 / ケラチン / マイクロRNA / シグナル伝達 / 予後マーカー |
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| Outline of Final Research Achievements |
To search for biomarkers to identify patients with a high risk of cSCC, we compared protein and microRNA (miRNA) expression profiles between transformed mouse keratinocytes and its metastatic derivatives. MiR-221/222, keratin 8 (K8)/18 were upregulated in metastatic derivatives.
We then examined whether miR-221/222 and K8/18 could serve as markers to identify high-risk patients. Of the 448 patients diagnosed/treated between 1978 and 2012 at Ryukyu University Hospital, 19 (4.2%) were metastatic, and 11 of which could be enrolled. Thus, we compared miR-221/222 and K8/18 expression between 11 metastatic and 11 non-metastatic patients in a matched-pair case-control study. MiR-221/222-double-positive staining and K8/18-double-positive staining were highly significantly associated with metastasis (each p=0.004, binomial exact test). To our knowledge, this is the first report on the potential utility of miR-221/222 and K8/18 as biomarkers for high-risk cSCCs.
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Report
(5 results)
Research Products
(1 results)
