The role of IL-33 and its reseptor in contact hypersensitivity reaction
| Project/Area Number |
23591652
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
KOMINE Mayumi 自治医科大学, 医学部, 准教授 (00282632)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | IL-33 / ケラチノサイト / 発現調節 / インターフェロンγ / ＵＶＢ / 表皮細胞 / ＩＬ－３３ / 炎症性皮膚疾患 / ＩＦＮγ / ＩＬ－１７ / ケモカイン / シグナル伝達 |
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| Research Abstract |
We investigated the hypersensitivity reaction to FITC and oxazolone in wild type and soluble ST2 transgenic mice. There were no statistical differences in ear thickness and histopathological observasion in these mice. UVB, IFNg, and IL-17 induced IL-33 expression in protein and mRNA levels in cultured normal human epidermal keratinocytes (NHEKs). The induction of IL-33 expression in NHEKs was dependent on EGFR and ERK with these stimulations. In case of IL-17 and IFNg, the induction was dependent also on STAT1 and JAK. We have identified three distinct exon1s in the upstream of exon2. The putative promoter regions of IL-33 were cloned into luciferase vectors, and we identified active promoter region in the 5'flanking region of second exon1 (exon1b). This promoter region was active in NHEKs, normal human dermal fibroblasts, and human umbilical vein endothelial cells. The promoter activity was induced by the stimulation with IFNg and IL-17, consistent to our protein and mRNA results.
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] Sustained elevation of interleukin-33 in sera and synovial fluids from patients with rheumatoid arthritis non-responsive to anti-tumor necrosis factor : possible association with persistent IL-1βsignaling and a poor clinical response2012
Author(s)
Matsuyama Y, Okazaki H, Hoshino M, Onishi S, Kamata Y, Nagatani K, Nagashima T, Iwamoto M, Yoshio T, Ohto-Ozaki H, Tamemoto H, Komine M, Sekiya H, Tominaga S, Minota S
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Journal Title
Rheumatol Int
Volume: 32(5)
Pages: 1397-401
Related Report
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[Journal Article] Novel splice variants of IL-33: Differential expression in normal and transformed cells2012
Author(s)
Tsuda, H., Komine, M., Karakawa, M., Etoh, T., Tominaga, S., and Ohtsuki, M
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Journal Title
J. Invest. Dermatol
Volume: 132
Issue: 11
Pages: 2661-2664
DOI
Related Report
Peer Reviewed
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[Journal Article] The levels of soluble ST2 in sera and bullous fluid from patients with bullous pemphigoid.2012
Author(s)
Wakatabi, K., Komine, M., Meephansan, J., Matsuyama, Y., Tsuda, H., Tominaga, S., and Ohtsuki, M.
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Journal Title
Eur. J. Dermatol
Volume: 22
Issue: 3
Pages: 333-336
DOI
Related Report
Peer Reviewed
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