elucidation of the molecular mechanisms about the protective effect of hydrogen against renal ischemia-reperfusion injury

• Project/Area Number: 23592258
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Nagasaki University
• Principal Investigator: MIYOSHI hiroshi 長崎大学, 大学病院, 助教 (90332858)
• Co-Investigator(Kenkyū-buntansha): MAEKAWA Takuji 長崎大学病院, 麻酔科, 講師 (10336167)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 腎虚血再灌流 / 水素 / 腎臓 / 保護

Research Abstract

Organ ischemia-reperfusion injury in a living body, greatly changed the life prognosis. The studies were conducted with a focus on the renal protective effect of hydrogen as an agent with the organ protective effects. Use the Wistar rat, anesthesia enforcement, make tracheal intubation, artificial respiration, after exposure to the kidney on both sides side abdominal incision, to clamp en bloc renal artery, renal vein, ureter. Ischemic time is 40 minutes. Administration of hydrogen was performed by dividing the low concentration and high concentration group. After that, I closed the wound. Blood samples were collected after 24 hours, and measured blood urea nitrogen, and creatinine. By hydrogen administration, increased blood urea nitrogen, creatinine can be suppressed has been found.