Analysis of Dkk-1 function in castrate-resistant prostate cancer
| Project/Area Number |
23592339
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kumamoto University |
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Principal Investigator |
KAWANO Yoshiaki 熊本大学, 大学院生命科学研究部, 助教 (30593793)
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| Co-Investigator(Kenkyū-buntansha) |
ETO Masatoshi 熊本大学, 生命科学研究部, 教授 (90315078)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 前立腺癌 |
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| Research Abstract |
Amongst prostate cancer cell lines, we found Dkk1 was expressed in the culture supernatant of castrate-resistant PC-3 cells by western blot. We established LNCaP cell line inducibly expressing Dkk1 in a tetracycline dependent manner. However, those cells did not exhibit castration resistance in vitro by inducible Dkk1 expression. On the other hand, invasion of PC-3 in boyden chamber was significantly inhibited by Dkk1 knockdown by siRNA. These results suggest that Dkk1 expression does not directly contribute to acquisition of castration resistance but is rather involved in invasiveness of castrate resistant prostate cancer cells.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Enhancement of human cancer cell motility and invasiveness by anaphylatoxin C5a via aberrantly expressed C5a-receptor (CD88)2013
Author(s)
Hidetoshi Nitta, Yoshihiro Wada, Yoshiaki Kawano, Yoji Murakami, Atsushi Irie, Keisuke Taniguchi, Ken Kikuchi, Gen Yamada, Kentaro Suzuki, Jiro Honda, Masayo Wilson-Morifuji, Norie Araki, Masatoshi Eto, Hideo Baba and Takahisa Imamura
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Journal Title
Clin Cancer Res
Volume: 19(8)
Pages: 2004-2013
Related Report
Peer Reviewed
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[Journal Article] Severe bleeding tendency caused by a rare complication of excessive fibrinolysis with disseminated intravascular coagulation in a 51-year-old Japanese man with prostate cancer : a case report2012
Author(s)
Wada Y, Uchiba M, Kawano Y, Kai N, Takahashi W, Honda J, Tanoue K, Maeda Y, Murakami Y, Eto M, Imamura T
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Journal Title
J Med Case Rep
Volume: 19(4)
Pages: 284-295
Related Report
Peer Reviewed
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[Journal Article] Severe bleeding tendency caused by excessive fibrinolysis with disseminated intravascular coagulation in a prostate cancer patient: a case report.2012
Author(s)
Wada, Y., Uchiba, M., Kawano, Y., Kai, N., Takahashi, W., Honda, J., Tanoue, K., Maeda, Y., Murakami, Y., Eto, M., Imamura, T.
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Journal Title
J Med Case Rep
Volume: 6
Pages: 378-378
DOI
Related Report
Peer Reviewed
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[Presentation] Loss of Dickkopf-3 expression impairs prostate acinar morphogenesis via aberrant TGF-β/Smad signalling activation.2013
Author(s)
Y. Kawano, D. Romero, N. Bengoa, N. Maltry, M. Walker, M. Eto, J. Waxman, C. Niehrs, R. Kypta
Organizer
The Annual Meeting of American Urological Association
Place of Presentation
San Diego, CA, USA
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