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Involvements of TRPV1 and TRPV4 channels in regulatory function to maintain bladder stability during urine storage: integrated analysis of behavior and reflex

Research Project

Project/Area Number 23592361
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Urology
Research InstitutionUniversity of Yamanashi

Principal Investigator

MOCHIZUKI Tsutomu  山梨大学, 医学工学総合研究部, 医学研究員 (50377496)

Co-Investigator(Kenkyū-buntansha) YOSHIYAMA Mitsuharu  山梨大学, 医学工学総合研究部, 教授 (20422694)
TAKEDA Masayuki  山梨大学, 医学工学総合研究部, 教授 (80197318)
NAKAGOMI Hiroshi  山梨大学, 医学部附属病院, 助教 (80418714)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords膀胱上皮 / TRPV4 / ATP / メカノセンサー / TRPV4 / Piezo1 / TRPV4 / 求心性シグナル伝達 / 蓄尿障害
Research Abstract

These studies using transient receptor potential vanilloid (TRPV)1-knock out (KO) mice and TRPV4-KO mice were conducted to examine functional roles of TRPV1 and TRPV4 channels in regulation of lower urinary tract activity. Analysis of voiding behavior with novel metabolic cages showed that TRPV4-KO mice have markedly higher voiding frequency and TRPV1-KO mice exhibit similar VFD.Evaluation of reflex micturition under decerebrate unanesthetized conditions using in vivo cystometrograms revealed that both TRPV1-KO and TRPV4-KO mice exhibit a large number of non-voiding bladder contractions (NVCs) whereas WT hardly show NVCs. These results suggested the possibilities that TRPV1 and TRPV4 channels are involved in modulation to stabilize detrusor activity during urine storage and that conscious TRPV4-KO mice are more vulnerable to NVCs, which are transient but repeatedly-bothering events, than TRPV1-KO mice, therefore leading to urinary incontinence more easily.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (13 results)

All 2014 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (6 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Functional role for Piezo1 in stretch-evoked Ca2+ influx and ATP release in urothelial cell cultures2014

    • Author(s)
      Tatsuya Miyamoto, Tsutomu Mochizuki, Hiroshi Nakagomi, Satoru Kira, Masaki Watanabe, Yasunori Takayama, Yoshiro Suzuki, Schuichi Koizumi, Masayuki Takeda and MakotoTominaga
    • Journal Title

      J Biol Chem

      Volume: (Epub ahead of print)

    • Related Report
      2013 Final Research Report
  • [Journal Article] Functional role for Piezo1 in stretch-evoked Ca 2+ influx and ATP release in urothelial cell cultures.2014

    • Author(s)
      Miyamoto T, Mochizuki T, Nakagomi H, Kira S, Watanabe M, Takayama Y, Suzuki Y, Koizumi S, Takeda M, Tominaga M.
    • Journal Title

      J Biol Chem.

      Volume: 53 Pages: 1230-1238

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 膀胱上皮におけるTRPVチャネルとATP放出メカニズム2013

    • Author(s)
      中込宙史、望月 勉、武田正之
    • Journal Title

      膀胱上皮におけるTRPVチャネルとATP放出メカニズム

      Volume: vol.20 Pages: 7-14

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 膀胱上皮におけるTRPVチャネルとATP放出メカニズム2012

    • Author(s)
      中込宙史、望月勉、武田正之
    • Journal Title

      排尿障害プラクティス

      Volume: vol.20, No.1 Pages: 7-14

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] VNUT(Vesicular Nucleotide Transporter)を介する膀胱上皮からのATP放出メカニズム2011

    • Author(s)
      中込宙史、望月勉、芳山充晴、宮本達也吉良聡、川原和也、荒木勇雄、小泉修一森山芳則、武田正之
    • Journal Title

      日本排尿機能学会誌

      Volume: Vol.22(1) Pages: 121-121

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 腎平滑筋種の1例2011

    • Author(s)
      望月勉
    • Organizer
      第78回日本泌尿器科学会山梨地方会
    • Place of Presentation
      山梨
    • Related Report
      2013 Final Research Report
  • [Presentation] 新規のマウス用排尿代謝ケージ構築ならびに各種パラメーターについて~野生型マウスとTRPV4ノックアウトマウスとの比較~2011

    • Author(s)
      望月勉
    • Organizer
      第18回日本排尿機能学会
    • Place of Presentation
      福井
    • Related Report
      2013 Final Research Report
  • [Presentation] TRPV4-DEFICIENT MICE EXHIBIT INCREASED FREQUENCY OF VOIDING : ~EVALUATION OF MICTURITION BEHAVIOUR USING NOVEL METABOLIC CAGE SYSTEM~2011

    • Author(s)
      Tsutomu Mochizuki
    • Organizer
      International Continence Society
    • Place of Presentation
      イギリス
    • Related Report
      2013 Final Research Report
  • [Presentation] 新規ディバイス排尿用代謝ケージを用いたマウス排尿における各種パラメーターについて~野生型マウスとTRPVノックアウトマウスとの比較~2011

    • Author(s)
      望月勉
    • Organizer
      第99回日本泌尿器科学会総会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Final Research Report
  • [Presentation] TRPV4-KO mice exhibit increased voiding frequency2011

    • Author(s)
      Tsutomu Mochizuki
    • Organizer
      ICS41st Annual Meeting
    • Place of Presentation
      Scotish Exhibition&conference Center(Glasgow, U.K)
    • Related Report
      2011 Research-status Report
  • [Presentation] 排尿用代謝ケージを用いたマウス排尿における各種パラメータについて2011

    • Author(s)
      望月勉
    • Organizer
      日本排尿機能学会
    • Place of Presentation
      ホテルフジタ福井(福井県福井市)
    • Related Report
      2011 Research-status Report
  • [Book] 排尿障害プラクティス2012

    • Author(s)
      中込宙史、望月勉、武田正之
    • Total Pages
      8
    • Publisher
      メディカルレビュー社
    • Related Report
      2012 Research-status Report
  • [Remarks] なし

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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