| Project/Area Number |
23592390
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
SUGAWARA Junichi 東北大学, 東北メディカル・メガバンク機構, 教授 (60280880)
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| Co-Investigator(Kenkyū-buntansha) |
OKAE Hiroaki 東北大学, 医学系研究科, 助教 (10582695)
HIURA Hitoshi 東北大学, 医学系研究科, 助教 (70451523)
ARIMA Takahiro 東北大学, 医学系研究科, 教授 (80253532)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 胎盤幹細胞 / エピジェネティクス / 細胞系譜 |
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| Research Abstract |
The increased expression of Cdx2, and more differentiated embryonic body tissue to the blastocyst stage, extra-embryonic tissue to differentiate into placental tissue (TE) determines the cell fate. In this study, using 1) placental stem (TS) cells, and subjected to analysis for histone modification and DNA methylation of Cdx2 gene promoter region. As a result, the DNA methylation of the promoter region of the gene Cdx2 be low methylation status, that histone modifications are also active was found. As a result of using a TS cell line knockdown 2) of the Cdx2 gene was observed, were analyzed for cell cycle and morphological changes, G1 arrest was observed and reduced growth potential. From this fact, the differentiation and maintenance of TS cells, that Cdx2 gene plays an important role has been suggested.
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