Development of Lactobacillus-based therapeutic vaccine against Human papillomavirus for treatment of CIN3 patients

• Project/Area Number: 23592436
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: The University of Tokyo
• Principal Investigator: KAWANA kei 東京大学, 医学部附属病院, 准教授 (60311627)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 子宮頸癌 / ヒトパピローマウイルス（HPV） / HPV分子標的がん免疫療法 / 粘膜免疫 / 経口ワクチン / HPV治療ワクチン / 漢方アジュバント / 乳酸菌 / 子宮頚癌前癌病変 / 癌ワクチン / アジュバント / HPV / 乳酸菌ワクチン / E7 / 漢方薬

Outline of Final Research Achievements

Cervicalcancer and its precursor lesion,cervicalintraepithelial neoplasia grade3(CIN3)are a mucosal precancerous lesion caused by high-riskHPV.Induction ofimmunological clearance ofCIN3 by targetingHPV antigens is a strategy for CIN3therapy.We've evaluated the clinical efficacy of an attenuated Lactobacillus casei expressing HPV16E7,GLBL101c,in patients with HPV16-associatedCIN3.GLBL101c had a regression onCIN3to CIN2,but the efficacy remained partial.So we developed next generation E7-expressing Lactobacillus-basedvaccine to improve induction ofmucosalimmune responses to HPVE7.
1)TheJapanese herbalmedicines,JuzenTaihoTo(JTT)/HochuEkkiTo(HET)showed the synergy ofan oralLactobacillus-based vaccine on mucosalimmune responses.2)0.3μgofE7in1.2x10~8cells ofLactobacilluscasei wasthe most effective dose forimmunological inducibility with 2-fold higher thanthe existing,GLBL101c.The optimized newcancer-vaccine with increased pharmacologicaleffect will be a reliable drug for treatmentofCIN2-3.

Research Products

• [Journal Article] Characterization of novel transcripts of human papillomavirus type 16 using CAGE technology (2015)
  • Author(s): Taguchi A, Nagasaka K, Kawana K, Hashimoto K, Kusumoto-Matsuo R, Plessy C, Miranda T, Nakamura H, Bonetti A, Oda K, Kukimoto I, Carninci P, Banks L, Osuga Y, Fujii T,
  • Journal Title: J Virol Volume: 89 Issue: 4 Pages: 2448-2452
  • DOI: 10.1128/jvi.03433-14
  • Peer Reviewed / Open Access
• [Journal Article] Oral vaccination against HPV E7 for treatment of cervical intraepithelial neoplasia grade 3 (CIN3) elicits E7-specific mucosal immunity in the cervix of CIN3 patients (2014)
  • Author(s): Kawana K, Adachi K, Kojima S, Taguchi A, Tomio K, Yamashita A, Nishida H, Nagasaka K, Arimoto T, Yokoyama T, Wada-Hiraike O, Oda K, Sewaki T, Osuga Y, Fujii T
  • Journal Title: Vaccine Volume: 32 Issue: 47 Pages: 6233-6239
  • DOI: 10.1016/j.vaccine.2014.09.020
  • Peer Reviewed
• [Journal Article] Positive peritoneal cytology at interval debulking surgery is independent poor prognostic factor in patients with stage T3c advanced ovarian carcinoma (2014)
  • Author(s): Nagasaka K, Kawana K, Tomio K, Eguchi S, Miura S, Tsuruga T, Mori-Uchino M, Tanikawa M, Tsukazaki T, Sone K, Adachi K, Matsumoto Y, Arimoto T, Oda K, Osuga Y, Fujii T
  • Journal Title: J Obstet Gynecol Res Volume: Nov. 25 Issue: 5 Pages: 554-560
  • DOI: 10.1111/jog.12616
  • Peer Reviewed
• [Journal Article] Human papillomavirus genotype distribution in cervical intraepithelial neoplasia grade 2/3 and invasive cervical cancer in Japanese women (2014)
  • Author(s): Azuma Y, Kusumoto-Matsuo R, Takeuchi F, Uenoyama A, Kondo K, Tsunoda H, Nagasaka K, Kawana K, Morisada T, Iwata T, Aoki D, Kukimoto I
  • Journal Title: Japan J Clin Oncol, Volume: 44(10) Issue: 10 Pages: 910-7
  • DOI: 10.1093/jjco/hyu112
  • Peer Reviewed / Open Access
• [Journal Article] Matrix metalloproteinase (MMP)-9 in cancer-associated fibroblasts (CAFs) is suppressed by omega-3 polyusaturated fatty acids in vitro and in vivo. (2014)
  • Author(s): Taguchi A, Kawana K, Tomio K, Yamashita A, Isobe Y, Nagasaka K, Koga K, Inoue T, Nishida H, kojima S, Adachi K, Matsumoto Y, Arimoto T, Wada-Hiraike O, Oda K, kang JX, Arai H, Arita M, Osuga Y, Fujii T.
  • Journal Title: PLoS One Volume: 27 Issue: 2 Pages: e89605-e89605
  • DOI: 10.1371/journal.pone.0089605
  • Peer Reviewed
• [Journal Article] Resveratrol suppresses inflammatory responses in endometrial stromal cells derived from endometriosis: a possible role of the sirtuin 1 pathway. (2014)
  • Author(s): Taguchi A, Wada-Hiraike O, Kawana K, Koga K, Yamashita A, Shirane A, Urata Y, Kozuma S, Osuga Y, Fujii T,
  • Journal Title: 10.1111/jog.12252 Volume: 40 Pages: 770-778
  • Peer Reviewed
• [Journal Article] High-risk HPV correlates with recurrence after laser ablation for treatment of patients with CIN3: a long-term follow-up retrospective study, (2014)
  • Author(s): Inaba K, Nagasaka K, Kawana K, Arimoto T, Matsumoto Y, Tsuruga T, Mori-Uchino M, Miura S, Sone K, Oda K, Nakagawa S, Yano T, Kozuma S, Fujii T
  • Journal Title: J Obstet Gynaecol Res Volume: 40 Issue: 2 Pages: 554-60
  • DOI: 10.1111/jog.12196
  • Peer Reviewed
• [Journal Article] Anti-tumor activity of olaparib, a poly (ADP-ribose) polymerase (PARP) inhibitor, in cultured endometrial carcinoma cells. (2014)
  • Author(s): A. Miyasaka, K. Oda, Y. Ikeda, O. Wada-Hiraike, T. Kashiyama, A. Enomoto, N. Hosoya, T. Koso, T. Fukuda, K. Inaaba, K. Sone, Y. Uehara, R. Kurikawa, K. Nagasaka, Y.Matsumoto, T. Arimoto, S. Nakagawa, H. Kuramoto, K. Miyagawa, T. Yano, K. Kawana, Y. Osuga, T. Fujii.
  • Journal Title: BMC Cancer Volume: - Issue: 1 Pages: 179-179
  • DOI: 10.1186/1471-2407-14-179
  • Peer Reviewed
• [Journal Article] High-risk HPV correlates with recurrence after laser ablation for treatment of patients with CIN3: a long-term follow-up retrospective study, (2014)
  • Author(s): Inaba K, Nagasaka K, Kawana K, Arimoto T, Matsumoto Y, Tsuruga T, Mori-Uchino M, Miura S, Sone K, Oda K, Nakagawa S, Yano T, Kozuma S, Fujii T.
  • Journal Title: The journal of obstetrics and gynaecology research Volume: 40 Pages: 554-60
  • Peer Reviewed
• [Journal Article] HPV分子を標的とした粘膜免疫を介したがん免疫療法の開発 (2014)
  • Author(s): 川名 敬
  • Journal Title: 診断と治療社 Volume: 81 Pages: 210-216
• [Journal Article] PDZ domains and viral infection: versatile potentials of HPV-PDZ interactions in relation to malignancy. (2013)
  • Author(s): Nagasaka K, Kawana K, Osuga Y, Fujii T.
  • Journal Title: BioMed research international Volume: 2013 Pages: 369712-369712
  • DOI: 10.1155/2013/369712
  • Peer Reviewed
• [Journal Article] A novel interaction between hScrib and PP1γ downregulates ERK signaling and suppresses oncogene-induced cell transformation (2013)
  • Author(s): Nagasaka K, Seiki T, Yamashita A, Massimi P, Subbaiah VK, Thomas M, Kranjec C, Kawana K, Nakagawa S, Yano T, Taketani Y, Fujii T, Kozuma S, Banks L
  • Journal Title: PLosOne Volume: E-pub Issue: 1 Pages: 53752-53760
  • DOI: 10.1371/journal.pone.0053752
  • Peer Reviewed
• [Journal Article] Association between carotenoids and outcome of cervical intraepithelial neoplasia: a prospective cohort study. (2013)
  • Author(s): Fujii T
  • Journal Title: Int J Clin Oncol Volume: in press Issue: 6 Pages: 1091-101
  • DOI: 10.1007/s10147-012-0486-5
  • Peer Reviewed
• [Journal Article] Sequential effects of the proteasome inhibitor bortezomib and chemotherapeutic agents in uterine cervical cancer cell lines. (2013)
  • Author(s): Miyamoto Y, Nakagawa S, Wada-Hiraike O, Seiki T, Tanikawa M, Hiraike H, Sone K, Nagasaka K, Oda K, Kawana K, Nakagawa K, Fujii T, Yano T, Kozuma S, Taketani Y.
  • Journal Title: Oncol Rep. Volume: 29(1) Issue: 1 Pages: 51-7
  • DOI: 10.3892/or.2012.2072
  • Peer Reviewed
• [Journal Article] The prevalence of cervical regulatory T cells in HPV-related cervical intraepithelial neoplasia (CIN) correlates inversely with spontaneous regression of CIN (2013)
  • Author(s): Kojima S, Kawana K, Tomio K, Yamashita A, Taguchi A, Nagamatsu T, Nagasaka K, Matsumoto Y, Arimoto T, Oda K, Wada-Hiraike O, Yano T, Taketani Y, Fujii T, Schust DJ, Kozuma S
  • Journal Title: Am J Reprod Immunol, Volume: 69 Issue: 2 Pages: 134-144
  • DOI: 10.1111/aji.12030
  • Peer Reviewed
• [Journal Article] 新たなHPVワクチンの開発 (2013)
  • Author(s): 川名 敬
  • Journal Title: 臨床婦人科産科 Volume: 67 Pages: 790-797
• [Journal Article] Effective treatment of pelvic lymphocele by lymphaticovenular anastomosis (2013)
  • Author(s): Todokoro T, Furniss D, Oda K, Kawana K, Narushima M, Mihara M, Kikuchi K, Hara H, Yano T, Koshima I
  • Journal Title: Gynecol Oncol Volume: E-pub Issue: 2 Pages: 209-214
  • DOI: 10.1016/j.ygyno.2012.11.014
  • Peer Reviewed
• [Journal Article] Association between carotenoids and outcome of cervical intraepithelial neoplasia: a prospective cohort study (2013)
  • Author(s): Fujii T, Takatsuka N, Nagata C, Matsumoto K, Oki A, Furuta R, Maeda H, Yasugi T, Kawana K, Mitsuhashi A, Hirai Y, Iwasaka T, Yaegashi N, Watanabe Y, Nagai Y, Kitagawa T, Yoshikawa H
  • Journal Title: Int J Clin Oncol Volume: E-pub
  • Peer Reviewed
• [Journal Article] The influence of infertility treatment on the prognosis of endometrial cancer and atypical complex endometrial hyperplasia (2013)
  • Author(s): Ichinose M, Fujimoto A, Osuga Y, Minaguchi T, Kawana K, Yaho T, Kozuma S.
  • Journal Title: Int J Gynecol Cancer Volume: 23 Issue: 2 Pages: 288-293
  • DOI: 10.1097/igc.0b013e31827c18a1
  • Peer Reviewed
• [Journal Article] Therapeutic human papillomavirus (HPV) vaccines: a novel approach (2012)
  • Author(s): Kawana K, Adachi K, Kojima S, Kozuma S, Fujii T
  • Journal Title: Open Virol J Volume: 6 Issue: 1 Pages: 264-269
  • DOI: 10.2174/1874357901206010264
  • Peer Reviewed
• [Journal Article] Adjuvant effect of Japanese herbal medicines on the mucosal type 1 immune response to human papillomavirus (HPV) E7 in mice immunized orally with Lactobacillus-based therapeutic HPV vaccine in a synergistic manner. (2012)
  • Author(s): Taguchi A, Kawana K, Yokoyama T, Adachi K, Yamashita A, Tomio K, Kojima S, Oda K, Fujii T, Kozuma S
  • Journal Title: Vaccine Volume: 30 Issue: 36 Pages: 5368-5372
  • DOI: 10.1016/j.vaccine.2012.06.027
  • Peer Reviewed
• [Journal Article] Genome-wide single nucleotide polymorphism arrays as a diagnostic tool in patients with synchronous endometrial and ovarian cancer. (2012)
  • Author(s): Ikeda Y, Oda K, Nakagawa S
  • Journal Title: Int J Gynecol Cancer Volume: 22(5） Issue: 5 Pages: 725-31
  • DOI: 10.1097/igc.0b013e31824c6ea6
  • Peer Reviewed
• [Journal Article] Genotype-dependent efficacy of a dual PI3K/mTOR inhibitor, NVP-BEZ235, and an mTOR inhibitor, RAD001, in endometrial carcinomas. (2012)
  • Author(s): Shoji K, Oda K, Nakagawa S,-Hiraike O, McCormick F, Aburatani H, Yano T, Kozuma S, Taketani Y.
  • Journal Title: PLoS One Volume: 7(5): Issue: 5 Pages: e37431-e37431
  • DOI: 10.1371/journal.pone.0037431
  • Peer Reviewed
• [Journal Article] Regulation of SIRT1 determines initial step of endometrial receptivity by controlling E-cadherin expression (2012)
  • Author(s): Shirane A, Wada-Hiraike O, Tanikawa M, Seiki T, Hiraike H, Miyamoto Y, Sone K, Hirano M, Oishi H, Oda K, Kawana K, Nakagawa S, Osuga Y, Fujii T, Yano T, Kozuma S, Taketani Y
  • Journal Title: Biochem Biophys Res Commun Volume: 424 Issue: 3 Pages: 604-10
  • DOI: 10.1016/j.bbrc.2012.06.160
  • Peer Reviewed
• [Journal Article] 12.Retreatment with nedaplatin in patients with recurrent gynecological cancer after the development of hypersensitivity reaction to carboplatin (2012)
  • Author(s): Arimoto T, Oda K, Nakagawa S, Kawana K, Tsukazaki T, Adachi K, Matsumoto Y, Yano T, Kozuma S, Taketani Y.
  • Journal Title: J Obstet Gynaecol Res Volume: 39 Pages: 336-40
  • Peer Reviewed
• [Journal Article] Low-grade endometrial stromal sarcoma developing in a postmenopausal woman under toremifene treatment for breast cancer (2012)
  • Author(s): Kashiyama T, Oda K, Kawana K, Arimoto T, Kanetaka Y, Takazawa Y, Maeda D, Nakagawa S, Yano T, Kozuma S.
  • Journal Title: J Obstet Gynaecol Res Volume: 39 Pages: 424-9
  • Peer Reviewed
• [Journal Article] A possible coagulation-independent mechanism for pregnancy 1 loss involving β2glycoprotein 1-dependent antiphospholipid antibodies and CD1d. (2012)
  • Author(s): Iwasawa Y, Kawana K, Fujii T, Schust DJ, Nagamatsu T, Kawana Y, Sayama S, Miura S, Matsumoto J, Adachi K, Hyodo H, Yamashita T, Kozuma S, Taketani Y
  • Journal Title: Am J Reprod Immunol Volume: 67 Pages: 54-65
  • Peer Reviewed
• [Journal Article] Introducing HPV vaccine and scaling up screening procedures to prevent deaths from cervical cancer in Japan: A cost-effectiveness analysis. (2012)
  • Author(s): Yamamoto N, Mori R, Jacklin P, Osuga Y, Kawana K, Shibuya K, Taketani Y
  • Journal Title: Br J Obstet and Gynecol, Volume: 119 Pages: 177-186
  • Peer Reviewed
• [Journal Article] Do neutralizing antibody responses generated by human papillomavirus infections favor a better outcome of low-grade cervical lesions? (2012)
  • Author(s): Ochi H, Matsumoto K, Kondo K, Oki A, Furuta R, Hirai Y, Yasugi T, Takatsuka N, Maeda H, Mitsuhashi A, Fujii T, Kawana K, Iwasaka T, Yaegashi N, Watanabe Y, et al
  • Journal Title: J Med Virol Volume: in-press
  • Peer Reviewed
• [Journal Article] Characterization of intraepithelial lymphocytes (IELs) residing in the cervical mucosa of patients with human papillomavirus (HPV)-infected intraepithelial neoplastic lesions. (2011)
  • Author(s): Kojima S, Kawana K, Fujii T, Yokoyama T, Miura S, Tomio K, Tomio A, Yamashita A, Adachi K, Sato H, Nagamatsu T, Schust DJ, Kozuma S, Taketani Y
  • Journal Title: Am J Reprod Immunol, Volume: 66 Pages: 435-443
  • Peer Reviewed
• [Journal Article] Interstitial pneumonitis induced by pegylated liposomal doxorubicin in a patient with recurrent ovarian cancer. (2011)
  • Author(s): Inaba K, Arimoto T, Hoya M, Kawana K, Nakagawa S, Kozuma S, Taketani Y
  • Journal Title: Med Oncol Volume: E-pub
  • Peer Reviewed
• [Journal Article] Second-line chemotherapy with docetaxel and carboplatin in paclitaxel and platinum-pretreated ovarian, fallopian tube, and peritoneal cancer. (2011)
  • Author(s): Arimoto T, Nakagawa S, Oda K, Kawana K, Yasugi T, Taketani Y
  • Journal Title: Med Oncol Volume: E-pub
  • Peer Reviewed
• [Journal Article] Subsequent risks for cervical precancer and cancer in women with low-grade squamous intraepithelial lesions unconfirmed by colposcopy-directed biopsy: Results from a multicenter, prospective, cohort study (2011)
  • Author(s): Matsumoto K, Hirai Y, Furuta R, Takatsuka N, Oki A, Yasugi T, Maeda H, Mitsuhashi A, Fujii T, Kawana K, Iwasaka T, Yaegashi N, Watanabe Y, Nagai Y, Kitagawa T, Yoshikawa H
  • Journal Title: Int J Clin Oncol Volume: E-pub
  • NAID: 10030951526
  • Peer Reviewed
• [Presentation] HPVを標的とした抗腫瘍、抗ウイルス免疫療法の新展開 (2014)
  • Author(s): 川名 敬
  • Organizer: 第62回日本ウイルス学会
  • Place of Presentation: 東京・横浜
  • Year and Date: 2014-11-10 – 2014-11-12
  • Invited
• [Presentation] ワークショップ CINに対するHPV分子標的経口癌ワクチンに関するTR研究 (2014)
  • Author(s): 川名 敬
  • Organizer: 第56回日本婦人科腫瘍学会
  • Place of Presentation: 宇都宮
  • Year and Date: 2014-07-17 – 2014-07-19
  • Invited
• [Presentation] CIN3に対する粘膜免疫を介した抗HPV分子標的癌免疫療法の第I/IIa相臨床試験の最終解析 (2014)
  • Author(s): 足立克之、川名 敬、長阪一憲、横山照史、江口聡子、冨尾賢介、松本陽子、有本貴英、織田克利、大須賀穣、藤井知行
  • Organizer: 第66回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2014-04-18 – 2014-04-20
• [Presentation] HPV感染症を見直すー基礎から臨床まで (2013)
  • Author(s): 川名 敬
  • Organizer: 日本性感染症学会
  • Place of Presentation: 長良川国際会議場
  • Invited
• [Presentation] HPV-associated cancer and development of a novel anti-cancer HPV therapeutic vaccine (2013)
  • Author(s): Kawana K
  • Organizer: 19th International Charles Heidelberger Symposium
  • Place of Presentation: 鹿児島
  • Invited
• [Presentation] HPVに対する腸管粘膜免疫を介した子宮頸癌治療ワクチンの開発 (2013)
  • Author(s): 川名 敬
  • Organizer: 日本薬学会
  • Place of Presentation: 横浜
  • Invited
• [Presentation] Current issues and future for prophylactic and therapeutic HPV vaccines, (2012)
  • Author(s): Kawana K
  • Organizer: 第10回日本臨床腫瘍学会 ワークショップ
  • Place of Presentation: 大阪
  • Invited
• [Presentation] 性感染症に対する粘膜免疫を介したワクチン開発 (2012)
  • Author(s): 川名 敬
  • Organizer: 第16回日本ワクチン学会
  • Place of Presentation: 横浜
  • Invited
• [Presentation] Development of novel HPV vaccines: broad-spectrum prophylactic and therapeutic (2011)
  • Author(s): Kawana K
  • Organizer: 第63回日本産科婦人科学会、日韓シンポジウム(招待講演)
  • Place of Presentation: 大阪
• [Presentation] Novel immunotherapy and the clinical trial for cervical cancer via mucosal immunity to human papillomavirus E7 (2011)
  • Author(s): Kawana K, Terufumi Yokoyama, Satoko Kojima, Shunsuke Nakagawa, Katsutoshi Oda, Tetsu Yano, Yuji Taketani
  • Organizer: 第70回日本癌学会
  • Place of Presentation: 名古屋
• [Presentation] 日本エイズ学会日本性感染症学会合同シンポジウム：婦人科領域における性感染症～HPVワクチンによる予防を含めて (2011)
  • Author(s): 川名敬、
  • Organizer: 日本性感染症学会(招待講演)
  • Place of Presentation: 東京
• [Presentation] Immunotherapy for cervical neoplasia through HPV E7-specific mucosal immunity
  • Author(s): Kawana K
  • Organizer: The 51th Annual meeting of Japanese Society of Clinical Oncology (JSCO2013), Kyoto, 2013
  • Place of Presentation: 国立京都国際会館
  • Invited
• [Presentation] Immunology of HPV infection and pathophysiology of cervical cancer
  • Author(s): Kawana K
  • Organizer: The 23rd Asian and Oceanic Congress of Obstetrics and Gynecology (AOCOG2013)
  • Place of Presentation: Centara Grand Bangkok Convention Center
  • Invited
• [Presentation] A novel approach: Immunotherapy for cervical intraepithelial neoplastic (CIN) lesions through HPV E7-specific mucosal immunity
  • Author(s): Kawana K
  • Organizer: The 12th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路夢舞台国際会議場
  • Invited
• [Presentation] HPVと子宮頸癌～HPVを標的とした創薬の臨床応用
  • Author(s): 川名 敬
  • Organizer: 第61回日本ウイルス学会
  • Place of Presentation: 神戸国際会議場
  • Invited
• [Patent(Industrial Property Rights)] 乳酸菌含有組成物、ＨＰＶ感染症及びＨＰＶ関連腫瘍の少なくともいずれかの治療用経口医薬組成物、及び粘膜免疫誘導剤 (2015)
  • Inventor(s): 川名 敬、五十君 靜信
  • Industrial Property Rights Holder: 川名 敬、五十君 靜信
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-017407
  • Filing Date: 2015-01-30
• [Patent(Industrial Property Rights)] 粘膜免疫賦活化剤及びHPV感染症治療用経口医薬組成物 (2013)
  • Inventor(s): 川名敬、田口歩
  • Industrial Property Rights Holder: 国立大学法人東京大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-138943
  • Filing Date: 2013-06-19
• [Patent(Industrial Property Rights)] 粘膜免疫賦活化剤及びHPV感染症治療用経口医薬組成物 (2012)
  • Inventor(s): 川名 敬
  • Industrial Property Rights Holder: 国立大学法人東京大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-138943
  • Filing Date: 2012-06-20