Ovarian cancer specific gene therapy by polymer-coated oncolytic adenovirus

• Project/Area Number: 23592453
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Ehime University
• Principal Investigator: HAMADA Katsuyuki 愛媛大学, 医学部附属病院, 講師 (90172973)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: オンコリティックアデノウイルス / 抗体 / ポリマー加工 / ポリエチレンイミン / コンドロイチン硫酸 / oncolytic adenovirus / immunogenecity / ovarian cancer / polyethylenimine / chondroitin sulfate / neutralizing antibody / 卵巣癌 / オンコリティックウイルス

Research Abstract

Because oncolytic adenovirus, AdE3-midkine is negatively charged, positively charged polyethylenimine (PEI) and negatively charged chondroitin sulfate were respectively layered on the surface of AdE3-midkine. In the antiadenovirus antibody, 12 layers with PEI and chondroitin sulfate showed the most potent antiproliferative effect. In the B6C3F1 syngeneic mouse model, respectively 90% and 60% of subcutaneous and intraperitoneal tumor growth were completely reduced.

Research Products

• [Journal Article] Biosafety studies of carrier cells infected with a replication-competent adenovirus introduced by IAI.3B promoter (2014)
  • Author(s): Hamada, K., Shirakawa, T., Terao, S., Gotoh, A., Tani, K., Huang, W
  • Journal Title: Molecular Therapy--Methods & Clinical Development Volume: (In print)
• [Journal Article] Biosafety studies of carrier cells infected with a replication-competent adenovirus introduced by IAI.3B promoter. (2014)
  • Author(s): 2. Hamada, K., Shirakawa, T., Terao, S., Gotoh, A., Tani, K., Huang, W.
  • Journal Title: Molecular Therapy--Methods & Clinical Development Volume: in print
  • Peer Reviewed
• [Journal Article] (2013) Concurrent chemoradiotherapy with nedaplatin in patients with stage IIA to IVA cervical carcinoma (2013)
  • Author(s): Fujioka, T., Yasuoka, T., Koizumi, M., Tanaka, H., Hashimoto, H., Nabeta, M., Koizumi, K., Matsubara, Y., Hamada, K., Matsubara, K., Katayama, T., Nawa, A
  • Journal Title: Molecular and clinical oncology Volume: 1 Pages: 165-170
• [Journal Article] Concurrent chemoradiotherapy with nedaplatin in patients with stage IIA to IVA cervical carcinoma. (2013)
  • Author(s): Fujioka, T., Yasuoka, T., Koizumi, M., Tanaka, H., Hashimoto, H., Nabeta, M., Koizumi, K., Matsubara, Y., Hamada, K., Matsubara, K., Katayama, T., Nawa, A.
  • Journal Title: Mol Clin Oncol. Volume: 1 Pages: 165-170
  • DOI: 10.3892/mco.2012.27
  • Peer Reviewed
• [Journal Article] (2012) Oncolytic plasmid : A novel strategy for tumor immuno-gene therapy (2012)
  • Author(s): Yoshihara, C., Hamada, K., Kuroda, M. and Koyama, Y
  • Journal Title: Oncology Letters Volume: 3 Pages: 387-390
• [Journal Article] (2012) Antitumor effect of chondroitin sulfate-coated ternary GM-CSF plasmid complex for ovarian cancer (2012)
  • Author(s): Hamada, K., Yoshihara, C., Ito, T., Tani, K., Tagawa, M., Sakuragawa, N., Itoh, H. and Koyama, Y
  • Journal Title: Journal of Gene Medicine Volume: 14:
• [Journal Article] Improved gene transfer into bladder cancer cells using adenovirus vector containing RGD motif (2012)
  • Author(s): Hiwasa, K., Nagaya, H., Terao, S., Acharya, B., Hamada, K., Mizuguchi, H. and Gotoh, A
  • Journal Title: Anticancer research Volume: 32 Pages: 3137-3140
• [Journal Article] Oncolytic plasmid: A novel strategy for tumor immuno-gene therapy. (2012)
  • Author(s): CHIEKO YOSHIHARA, 他
  • Journal Title: Oncology Letters Volume: 3 Pages: 387-390
  • DOI: 10.3892/ol.2011.467
  • Peer Reviewed
• [Journal Article] Antitumor effect of chondroitin sulfate-coated ternary GMCSF plasmid complex for ovarian cancer (2012)
  • Author(s): Katsuyuki Hamada, 他
  • Journal Title: The Journal of Gene Medicine Volume: 14 Pages: 120-127
  • DOI: 10.1002/jgm.1647
  • Peer Reviewed
• [Journal Article] Improved gene transfer into bladder cancer cells using adenovirus vector containing RGD motif. (2012)
  • Author(s): Hiwasa, K., Nagaya, H., Terao, S., Acharya, B., Hamada, K., Mizuguchi, H. and Gotoh, A.
  • Journal Title: Anticancer research Volume: 32 Pages: 3137-3140
  • Peer Reviewed
• [Journal Article] (2011) Intravenous injection of irradiated tumor cell vaccine carrying oncolytic adenovirus suppressed the growth of multiple lung tumors in a mouse squamous cell carcinoma model (2011)
  • Author(s): Saito, A., Morishita, N., Mitsuoka, C., Kitajima, S., Hamada, K., Lee, K.M., Kawabata, M., Fujisawa, M. and Shirakawa, T
  • Journal Title: Journal of Gene Medicine Volume: 13 Pages: 353-361
• [Journal Article] Gene therapy for oral squamous cell carcinoma with IAI.3B promoter-driven oncolytic adenovirus-infected carrier cells (2011)
  • Author(s): Zhang, T., Hamada, K., Hyodo, M., Itoh, H., Tani, K., Goda, H., Nakashiro, K. and Hamakawa, H
  • Journal Title: Oncology Reports Volume: 25 Pages: 795-802
• [Journal Article] Intravenous injection of irradiated tumor cell vaccine carrying oncolytic adenovirus suppressed the growth of multiple lung tumors in a mouse squamous cell carcinoma model. (2011)
  • Author(s): Saito, A., Morishita, N., Mitsuoka, C., Kitajima, S., Hamada, K., Lee, K.M., Kawabata, M., Fujisawa, M. and Shirakawa, T.
  • Journal Title: Journal of Gene Medicine Volume: 13 Pages: 353-361
  • Peer Reviewed
• [Journal Article] Gene therapy for oral squamous cell carcinoma with IAI.3B promoter-driven oncolytic adenovirus-infected carrier cells. (2011)
  • Author(s): Zhang, T., Hamada, K., Hyodo, M., Itoh, H., Tani, K., Goda, H., Nakashiro, K. and Hamakawa, H.
  • Journal Title: Oncology Reports Volume: 24 Pages: 795-802
  • Peer Reviewed
• [Presentation] 卵巣がんに対するコンドロイチン硫酸およびポリエチレンイミンによる多重コーテイングを施行したオンコリティックアデノウィルスの遺伝子治療 (2013)
  • Author(s): 高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三朗、那波明宏
  • Organizer: 第55回日本婦人科腫瘍学会学術講演会
  • Place of Presentation: 京都
• [Presentation] midkineプロモーター導入オンコリティックアデノウイルスによるコンドロイチン硫酸ポリマー多重加工人工皮膜を用いた卵巣癌に対する抗腫瘍効果 (2013)
  • Author(s): 高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三朗、那波明宏
  • Organizer: 第35回日本バイオマテリアル学会大会
  • Place of Presentation: 東京
• [Presentation] 卵巣癌に対するコンドロイチン硫酸を用いたオンコリティックアデノウイルスのポリマー多重加工による抗腫瘍効果 (2013)
  • Author(s): 高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三朗、那波明宏
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 東京
• [Presentation] ポリマー多重加工処理によるオンコリティックウイルスの電子顕微鏡による評価 (2013)
  • Author(s): 高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三郎、那波明宏
  • Organizer: 第19回日本遺伝子治療学会学術総会
  • Place of Presentation: 岡山
• [Presentation] Cancer gene therapy with oncolytic adenovirus by cell and polymer processing (2013)
  • Author(s): Hamada, K., Takagi, K., Tani, K., Nawa, A.
  • Organizer: 第19回日本遺伝子治療学会学術総会
  • Place of Presentation: 岡山
• [Presentation] transfer of chondroitin sulfate-coated granulocyte macrophage-colony stimulating factor in ovarian cancer. The ASGCT 1 (2013)
  • Author(s): Hamada, K., Takagi, K., Yoshihara, C., Ito, T., Koyama, Y., Itoh, H., Nawa, A. Gene
  • Organizer: 6th Annual Meeting
  • Place of Presentation: Salt Lake City, UT, USA
• [Presentation] Gene transfer of chondroitin sulfate-coated granulocyte macrophage-colony stimulating (2013)
  • Author(s): Hamada, K., Takagi, K., Yoshihara, C., Ito, T., Koyama, Y., Itoh, H., Nawa, A.
  • Organizer: The ASGCT 16th Annual Meeting
  • Place of Presentation: Salt Lake City, USA
• [Presentation] ポリマー多重加工処理によるオンコリティックウイルスの電子顕微鏡による評価. (2013)
  • Author(s): 高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三郎、那波明宏.
  • Organizer: 第19回日本遺伝子治療学会学術総会
  • Place of Presentation: 岡山
• [Presentation] 卵巣癌に対するコンドロイチン硫酸を用いたオンコリティックアデノウイルスのポリマー多重加工による抗腫瘍効果． (2013)
  • Author(s): 高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三朗、那波明宏．
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 東京
• [Presentation] midkineプロモーター導入オンコリティックアデノウイルスによるコンドロイチン硫酸ポリマー多重加工人工皮膜を用いた卵巣癌に対する抗腫瘍効果． (2013)
  • Author(s): 高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三朗、那波明宏．
  • Organizer: 第35回日本バイオマテリアル学会大会
  • Place of Presentation: 東京
• [Presentation] 卵巣がんに対するコンドロイチン硫酸およびポリエチレンイミンによる多重コーテイングを施行したオンコリティックアデノウィルスの遺伝子治療. (2013)
  • Author(s): 高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三朗、那波明宏.
  • Organizer: 第55回日本婦人科腫瘍学会学術講演会
  • Place of Presentation: 京都
• [Presentation] 犬のがん遺伝子治療におけるオンコリティックアデノウイルス感染キャリアー細胞の体内動態 (2012)
  • Author(s): 鈴木基史、佐々悠木子、鈴木和彦、成田洋平、荒木陽一、濱田雄行、小林正行、岸本海織、高島一昭、山根義久、水谷哲也、伊藤博
  • Organizer: 第33回動物臨床医学会年次大会
  • Place of Presentation: 大阪
• [Presentation] ポリマー多重加工処理によるオンコリティックアデノウイルスの免疫原性克服と抗腫瘍効果の検討 (2012)
  • Author(s): 濱田雄行、高木香津子、芳原智恵子、小山義之、那波明宏
  • Organizer: 第71回日本癌学会
  • Place of Presentation: 札幌
• [Presentation] Gene therapy of chondroitin sulfate-coated ternary GM-CSF plasmid complex for ovarian cancer. American Society of Gene & Cell Therapy (2012)
  • Author(s): Hamada, K., Yoshihara, C., Ito, T., Koyama, Y., Itoh, H., Takagi, K., Nawa, A.
  • Organizer: 15th Annual Meeting.
  • Place of Presentation: Philadelphia, Pennsylvania
• [Presentation] Gene therapy of chondroitin sulfate-coated ternary GM-CSF plasmid complex for ovarian cancer. (2012)
  • Author(s): Hamada, K., Yoshihara, C., Ito, T., Koyama, Y., Itoh, H., Takagi, K., Nawa, A.
  • Organizer: American Society of Gene & Cell Therapy 15th Annual Meeting
  • Place of Presentation: Philadelphia, USA
• [Presentation] 頭頚部癌におけるIAI.3Bプロモーター導入オンコリティックアデノウイルス感染キャリアー細胞の抗腫瘍効果 (2011)
  • Author(s): 濱田雄行
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 名古屋
• [Presentation] ウィルス抗原遺伝子を用いたガン免疫治療の新しい考えかた (2011)
  • Author(s): 小山義之、芳原智恵子、黒田美奈子、濱田雄行
  • Organizer: 第21回バイオ・高分子シンポジウム
  • Place of Presentation: 吹田市
• [Presentation] Novel adenovirus complexes having artificial envelope for tumor gene therapy (2011)
  • Author(s): Yoshihara, C., Hamada, K., Koyama, Y
  • Organizer: 第17回日本遺伝子治療学会
  • Place of Presentation: 福岡
• [Presentation] (2011) Efficacy and biosafety test of carrier cell infected with oncolytic adenovirus (2011)
  • Author(s): Hamada, K., Zhang, T., Shirakawa, T., Huang, W
  • Organizer: 102th AACR
  • Place of Presentation: Orland Florida
• [Presentation] Carrier cells infected with IAI.3B promoter-driven oncolytic adenovirus overcome immunogenicity and induce complete tumor reduction in oral squamous carcinoma cells The ASGCT (2011)
  • Author(s): Hamada, K., Zhang, T., Nakashiro, K., Hamakawa, H
  • Organizer: 14th Annual Meeting
  • Place of Presentation: Seattle, WA, USA
• [Presentation] Carrier cells infected with IAI.3B promoter-driven oncolytic adenovirus overcome immunogenicity and induce complete tumor reduction in oral squamous carcinoma cells (2011)
  • Author(s): Hamada, K., Zhang, T., Nakashiro, K., Hamakawa, H.
  • Organizer: The ASGCT 14th Annual Meeting
  • Place of Presentation: Seattle, USA
• [Presentation] Efficacy and biosafety test of carrier cell infected with oncolytic adenovirus (2011)
  • Author(s): Hamada, K., Zhang, T., Shirakawa, T., Huang, W.
  • Organizer: 102th AACR
  • Place of Presentation: Orland, USA
• [Presentation] ovel adenovirus complexes having artificial envelope for tumor gene therapy. (2011)
  • Author(s): Yoshihara, C., Hamada, K., Koyama, Y.
  • Organizer: 第17回日本遺伝子治療学会
  • Place of Presentation: 福岡
• [Presentation] ウィルス抗原遺伝子を用いたガン免疫治療の新しい考えかた. (2011)
  • Author(s): 小山義之、芳原智恵子、黒田美奈子、濱田雄行.
  • Organizer: 第21回バイオ・高分子シンポジウム
  • Place of Presentation: 吹田市
• [Patent(Industrial Property Rights)] 新規細胞、それを用いた抗腫瘍効果の 誘導剤、癌の遺伝子治療用医薬および抗腫瘍 効果の誘導方法 (2014)
  • Inventor(s): 濱田雄行
  • Industrial Property Rights Holder: 愛媛大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-074181
  • Filing Date: 2014-03-31
  • Application Year: 2014
  • Overseas
• [Patent(Industrial Property Rights)] 新規細胞、それを用いた抗腫瘍効果の誘導剤、癌の遺伝子治療用医薬および抗腫瘍効果の誘導方法 (2014)
  • Inventor(s): 濱田雄行
  • Industrial Property Rights Holder: 濱田雄行
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-074181
  • Filing Date: 2014-03-31
  • Application Year: 2014