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Ovarian cancer specific gene therapy by polymer-coated oncolytic adenovirus

Research Project

Project/Area Number 23592453
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionEhime University

Principal Investigator

HAMADA Katsuyuki  愛媛大学, 医学部附属病院, 講師 (90172973)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsオンコリティックアデノウイルス / 抗体 / ポリマー加工 / ポリエチレンイミン / コンドロイチン硫酸 / oncolytic adenovirus / immunogenecity / ovarian cancer / polyethylenimine / chondroitin sulfate / neutralizing antibody / 卵巣癌 / オンコリティックウイルス
Research Abstract

Because oncolytic adenovirus, AdE3-midkine is negatively charged, positively charged polyethylenimine (PEI) and negatively charged chondroitin sulfate were respectively layered on the surface of AdE3-midkine. In the antiadenovirus antibody, 12 layers with PEI and chondroitin sulfate showed the most potent antiproliferative effect. In the B6C3F1 syngeneic mouse model, respectively 90% and 60% of subcutaneous and intraperitoneal tumor growth were completely reduced.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (42 results)

All 2014 2013 2012 2011

All Journal Article (14 results) (of which Peer Reviewed: 7 results) Presentation (26 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Biosafety studies of carrier cells infected with a replication-competent adenovirus introduced by IAI.3B promoter2014

    • Author(s)
      Hamada, K., Shirakawa, T., Terao, S., Gotoh, A., Tani, K., Huang, W
    • Journal Title

      Molecular Therapy--Methods & Clinical Development

      Volume: (In print)

    • Related Report
      2013 Final Research Report
  • [Journal Article] Biosafety studies of carrier cells infected with a replication-competent adenovirus introduced by IAI.3B promoter.2014

    • Author(s)
      2. Hamada, K., Shirakawa, T., Terao, S., Gotoh, A., Tani, K., Huang, W.
    • Journal Title

      Molecular Therapy--Methods & Clinical Development

      Volume: in print

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] (2013) Concurrent chemoradiotherapy with nedaplatin in patients with stage IIA to IVA cervical carcinoma2013

    • Author(s)
      Fujioka, T., Yasuoka, T., Koizumi, M., Tanaka, H., Hashimoto, H., Nabeta, M., Koizumi, K., Matsubara, Y., Hamada, K., Matsubara, K., Katayama, T., Nawa, A
    • Journal Title

      Molecular and clinical oncology

      Volume: 1 Pages: 165-170

    • Related Report
      2013 Final Research Report
  • [Journal Article] Concurrent chemoradiotherapy with nedaplatin in patients with stage IIA to IVA cervical carcinoma.2013

    • Author(s)
      Fujioka, T., Yasuoka, T., Koizumi, M., Tanaka, H., Hashimoto, H., Nabeta, M., Koizumi, K., Matsubara, Y., Hamada, K., Matsubara, K., Katayama, T., Nawa, A.
    • Journal Title

      Molecular and clinical oncology

      Volume: 1 Issue: 1 Pages: 165-170

    • DOI

      10.3892/mco.2012.27

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] (2012) Oncolytic plasmid : A novel strategy for tumor immuno-gene therapy2012

    • Author(s)
      Yoshihara, C., Hamada, K., Kuroda, M. and Koyama, Y
    • Journal Title

      Oncology Letters

      Volume: 3 Pages: 387-390

    • Related Report
      2013 Final Research Report
  • [Journal Article] (2012) Antitumor effect of chondroitin sulfate-coated ternary GM-CSF plasmid complex for ovarian cancer2012

    • Author(s)
      Hamada, K., Yoshihara, C., Ito, T., Tani, K., Tagawa, M., Sakuragawa, N., Itoh, H. and Koyama, Y
    • Journal Title

      Journal of Gene Medicine

      Volume: 14:

    • Related Report
      2013 Final Research Report
  • [Journal Article] Improved gene transfer into bladder cancer cells using adenovirus vector containing RGD motif2012

    • Author(s)
      Hiwasa, K., Nagaya, H., Terao, S., Acharya, B., Hamada, K., Mizuguchi, H. and Gotoh, A
    • Journal Title

      Anticancer research

      Volume: 32 Pages: 3137-3140

    • Related Report
      2013 Final Research Report
  • [Journal Article] Oncolytic plasmid : A novel strategy for tumor immuno-gene therapy2012

    • Author(s)
      CHIEKO YOSHIHARA, 他
    • Journal Title

      ONCOLOGY LETTERS

      Volume: 3 Issue: 2 Pages: 387-390

    • DOI

      10.3892/ol.2011.467

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Antitumor effect of chondroitin sulfate-coated ternary granulocyte macro phage-colony-stimulating factor plasmid complex for ovarian cancer2012

    • Author(s)
      Katsuyuki Hamada, 他
    • Journal Title

      JOURNAL OF GENE MEDICINE

      Volume: 14 Issue: 2 Pages: 120-127

    • DOI

      10.1002/jgm.1647

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Improved gene transfer into bladder cancer cells using adenovirus vector containing RGD motif.2012

    • Author(s)
      Hiwasa, K., Nagaya, H., Terao, S., Acharya, B., Hamada, K., Mizuguchi, H. and Gotoh, A.
    • Journal Title

      Anticancer research

      Volume: 32 Pages: 3137-3140

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] (2011) Intravenous injection of irradiated tumor cell vaccine carrying oncolytic adenovirus suppressed the growth of multiple lung tumors in a mouse squamous cell carcinoma model2011

    • Author(s)
      Saito, A., Morishita, N., Mitsuoka, C., Kitajima, S., Hamada, K., Lee, K.M., Kawabata, M., Fujisawa, M. and Shirakawa, T
    • Journal Title

      Journal of Gene Medicine

      Volume: 13 Pages: 353-361

    • Related Report
      2013 Final Research Report
  • [Journal Article] Gene therapy for oral squamous cell carcinoma with IAI.3B promoter-driven oncolytic adenovirus-infected carrier cells2011

    • Author(s)
      Zhang, T., Hamada, K., Hyodo, M., Itoh, H., Tani, K., Goda, H., Nakashiro, K. and Hamakawa, H
    • Journal Title

      Oncology Reports

      Volume: 25 Pages: 795-802

    • Related Report
      2013 Final Research Report
  • [Journal Article] Intravenous injection of irradiated tumor cell vaccine carrying oncolytic adenovirus suppressed the growth of multiple lung tumors in a mouse squamous cell carcinoma model.2011

    • Author(s)
      Saito, A., Morishita, N., Mitsuoka, C., Kitajima, S., Hamada, K., Lee, K.M., Kawabata, M., Fujisawa, M. and Shirakawa, T.
    • Journal Title

      Journal of Gene Medicine

      Volume: 13 Pages: 353-361

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gene therapy for oral squamous cell carcinoma with IAI.3B promoter-driven oncolytic adenovirus-infected carrier cells.2011

    • Author(s)
      Zhang, T., Hamada, K., Hyodo, M., Itoh, H., Tani, K., Goda, H., Nakashiro, K. and Hamakawa, H.
    • Journal Title

      Oncology Reports

      Volume: 24 Pages: 795-802

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 卵巣がんに対するコンドロイチン硫酸およびポリエチレンイミンによる多重コーテイングを施行したオンコリティックアデノウィルスの遺伝子治療2013

    • Author(s)
      高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三朗、那波明宏
    • Organizer
      第55回日本婦人科腫瘍学会学術講演会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] midkineプロモーター導入オンコリティックアデノウイルスによるコンドロイチン硫酸ポリマー多重加工人工皮膜を用いた卵巣癌に対する抗腫瘍効果2013

    • Author(s)
      高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三朗、那波明宏
    • Organizer
      第35回日本バイオマテリアル学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] 卵巣癌に対するコンドロイチン硫酸を用いたオンコリティックアデノウイルスのポリマー多重加工による抗腫瘍効果2013

    • Author(s)
      高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三朗、那波明宏
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] ポリマー多重加工処理によるオンコリティックウイルスの電子顕微鏡による評価2013

    • Author(s)
      高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三郎、那波明宏
    • Organizer
      第19回日本遺伝子治療学会学術総会
    • Place of Presentation
      岡山
    • Related Report
      2013 Final Research Report
  • [Presentation] Cancer gene therapy with oncolytic adenovirus by cell and polymer processing2013

    • Author(s)
      Hamada, K., Takagi, K., Tani, K., Nawa, A.
    • Organizer
      第19回日本遺伝子治療学会学術総会
    • Place of Presentation
      岡山
    • Related Report
      2013 Final Research Report
  • [Presentation] transfer of chondroitin sulfate-coated granulocyte macrophage-colony stimulating factor in ovarian cancer. The ASGCT 12013

    • Author(s)
      Hamada, K., Takagi, K., Yoshihara, C., Ito, T., Koyama, Y., Itoh, H., Nawa, A. Gene
    • Organizer
      6th Annual Meeting
    • Place of Presentation
      Salt Lake City, UT, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Gene transfer of chondroitin sulfate-coated granulocyte macrophage-colony stimulating2013

    • Author(s)
      Hamada, K., Takagi, K., Yoshihara, C., Ito, T., Koyama, Y., Itoh, H., Nawa, A.
    • Organizer
      The ASGCT 16th Annual Meeting
    • Place of Presentation
      Salt Lake City, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Cancer gene therapy with oncolytic adenovirus by cell and polymer processing.2013

    • Author(s)
      Hamada, K., Takagi, K., Tani, K., Nawa, A.
    • Organizer
      第19回日本遺伝子治療学会学術総会
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
  • [Presentation] ポリマー多重加工処理によるオンコリティックウイルスの電子顕微鏡による評価.2013

    • Author(s)
      高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三郎、那波明宏.
    • Organizer
      第19回日本遺伝子治療学会学術総会
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
  • [Presentation] 卵巣癌に対するコンドロイチン硫酸を用いたオンコリティックアデノウイルスのポリマー多重加工による抗腫瘍効果.2013

    • Author(s)
      高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三朗、那波明宏.
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] midkineプロモーター導入オンコリティックアデノウイルスによるコンドロイチン硫酸ポリマー多重加工人工皮膜を用いた卵巣癌に対する抗腫瘍効果.2013

    • Author(s)
      高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三朗、那波明宏.
    • Organizer
      第35回日本バイオマテリアル学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 卵巣がんに対するコンドロイチン硫酸およびポリエチレンイミンによる多重コーテイングを施行したオンコリティックアデノウィルスの遺伝子治療.2013

    • Author(s)
      高木香津子、濱田雄行、伊藤智子、芳原智恵子、小山義之、谷憲三朗、那波明宏.
    • Organizer
      第55回日本婦人科腫瘍学会学術講演会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] 犬のがん遺伝子治療におけるオンコリティックアデノウイルス感染キャリアー細胞の体内動態2012

    • Author(s)
      鈴木基史、佐々悠木子、鈴木和彦、成田洋平、荒木陽一、濱田雄行、小林正行、岸本海織、高島一昭、山根義久、水谷哲也、伊藤博
    • Organizer
      第33回動物臨床医学会年次大会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report
  • [Presentation] ポリマー多重加工処理によるオンコリティックアデノウイルスの免疫原性克服と抗腫瘍効果の検討2012

    • Author(s)
      濱田雄行、高木香津子、芳原智恵子、小山義之、那波明宏
    • Organizer
      第71回日本癌学会
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Gene therapy of chondroitin sulfate-coated ternary GM-CSF plasmid complex for ovarian cancer. American Society of Gene & Cell Therapy2012

    • Author(s)
      Hamada, K., Yoshihara, C., Ito, T., Koyama, Y., Itoh, H., Takagi, K., Nawa, A.
    • Organizer
      15th Annual Meeting.
    • Place of Presentation
      Philadelphia, Pennsylvania
    • Related Report
      2013 Final Research Report
  • [Presentation] Gene therapy of chondroitin sulfate-coated ternary GM-CSF plasmid complex for ovarian cancer.2012

    • Author(s)
      Hamada, K., Yoshihara, C., Ito, T., Koyama, Y., Itoh, H., Takagi, K., Nawa, A.
    • Organizer
      American Society of Gene & Cell Therapy 15th Annual Meeting
    • Place of Presentation
      Philadelphia, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] 頭頚部癌におけるIAI.3Bプロモーター導入オンコリティックアデノウイルス感染キャリアー細胞の抗腫瘍効果2011

    • Author(s)
      濱田雄行
    • Organizer
      第70回日本癌学会総会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Final Research Report
  • [Presentation] 頭頚部癌におけるIAI.3Bプロモーター導入オンコリティックアデノウイルス感染キャリアー細胞の抗腫瘍効果2011

    • Author(s)
      濱田雄行
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] ウィルス抗原遺伝子を用いたガン免疫治療の新しい考えかた2011

    • Author(s)
      小山義之、芳原智恵子、黒田美奈子、濱田雄行
    • Organizer
      第21回バイオ・高分子シンポジウム
    • Place of Presentation
      吹田市
    • Related Report
      2013 Final Research Report
  • [Presentation] Novel adenovirus complexes having artificial envelope for tumor gene therapy2011

    • Author(s)
      Yoshihara, C., Hamada, K., Koyama, Y
    • Organizer
      第17回日本遺伝子治療学会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] (2011) Efficacy and biosafety test of carrier cell infected with oncolytic adenovirus2011

    • Author(s)
      Hamada, K., Zhang, T., Shirakawa, T., Huang, W
    • Organizer
      102th AACR
    • Place of Presentation
      Orland Florida
    • Related Report
      2013 Final Research Report
  • [Presentation] Carrier cells infected with IAI.3B promoter-driven oncolytic adenovirus overcome immunogenicity and induce complete tumor reduction in oral squamous carcinoma cells The ASGCT2011

    • Author(s)
      Hamada, K., Zhang, T., Nakashiro, K., Hamakawa, H
    • Organizer
      14th Annual Meeting
    • Place of Presentation
      Seattle, WA, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Carrier cells infected with IAI.3B promoter-driven oncolytic adenovirus overcome immunogenicity and induce complete tumor reduction in oral squamous carcinoma cells2011

    • Author(s)
      Hamada, K., Zhang, T., Nakashiro, K., Hamakawa, H.
    • Organizer
      The ASGCT 14th Annual Meeting
    • Place of Presentation
      Seattle, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Efficacy and biosafety test of carrier cell infected with oncolytic adenovirus2011

    • Author(s)
      Hamada, K., Zhang, T., Shirakawa, T., Huang, W.
    • Organizer
      102th AACR
    • Place of Presentation
      Orland, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] ovel adenovirus complexes having artificial envelope for tumor gene therapy.2011

    • Author(s)
      Yoshihara, C., Hamada, K., Koyama, Y.
    • Organizer
      第17回日本遺伝子治療学会
    • Place of Presentation
      福岡
    • Related Report
      2011 Research-status Report
  • [Presentation] ウィルス抗原遺伝子を用いたガン免疫治療の新しい考えかた.2011

    • Author(s)
      小山義之、芳原智恵子、黒田美奈子、濱田雄行.
    • Organizer
      第21回バイオ・高分子シンポジウム
    • Place of Presentation
      吹田市
    • Related Report
      2011 Research-status Report
  • [Patent(Industrial Property Rights)] 新規細胞、それを用いた抗腫瘍効果の 誘導剤、癌の遺伝子治療用医薬および抗腫瘍 効果の誘導方法2014

    • Inventor(s)
      濱田雄行
    • Industrial Property Rights Holder
      愛媛大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-074181
    • Filing Date
      2014-03-31
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 新規細胞、それを用いた抗腫瘍効果の誘導剤、癌の遺伝子治療用医薬および抗腫瘍効果の誘導方法2014

    • Inventor(s)
      濱田雄行
    • Industrial Property Rights Holder
      濱田雄行
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-074181
    • Filing Date
      2014-03-31
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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