Analysis of structural changes in rat retinal layers by optical coherence tomography in glaucoma-related models

• Project/Area Number: 23592557
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Kanazawa University
• Principal Investigator: HIGASHIDE TOMOMI 金沢大学, 大学病院, 講師 (20291370)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ラット / 網膜 / 光干渉断層計 / 緑内障

Research Abstract

We examined whether our optical coherence tomography (OCT) for rat eyes is useful for quantitative evaluation of structural changes in the retinal layers in a model with intravitreal injection of endothelin-1 (ET-1) and for evaluation of neuroprotective effects of drugs. We found that the minimum dose of ET-1 to cause retinal injuries was 20 pmol, and that the thickness of the inner retinal layer was well correlated with the retinal ganglion cell loss. We further examined the effects of topical tafluprost applied once daily on the retinal injuries by 20 pmol ET-1 in a double blinded manner. Topical tafluprost reduced the thinning of the retinal nerve fiber layer and the inner retinal layer by OCT and retinal ganglion cell loss. These results indicate that our OCT system is useful for evaluation of neuroprotective effects in rat glaucoma –related models.

Research Products

• [Journal Article] OCT evaluation of neuroprotective effects of tafluprost on retinal injury after intravitreal injection of endothelin-1 in the rat eye. (2014)
  • Author(s): Nagata A, Omachi K, Higashide T, Shirae S, Shimazaki A, Nakamura M, Ishida N, Sugiyama K.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 55 Issue: 2 Pages: 1040-1047
  • DOI: 10.1167/iovs.13-13056
  • Peer Reviewed
• [Journal Article] Structural and functional assessment by hemispheric asymmetry testing of the macular region in preperimetric glaucoma (2014)
  • Author(s): Kawaguchi C, Nakatani Y, Ohkubo S, Higashide T, (他2名)
  • Journal Title: Jpn J Ophthalmol Volume: 58 Issue: 2 Pages: 197-204
  • DOI: 10.1007/s10384-013-0293-1
  • NAID: 210000180148
  • Peer Reviewed
• [Journal Article] Effect of cataract and its removal on ganglion cell complex thickness and peripapillary retinal nerve fiber layer thickness measurements by fourier-domain optical coherence tomography (2013)
  • Author(s): Nakatani Y, Higashide T, (他3名)
  • Journal Title: J Glaucoma Volume: 22 Issue: 6 Pages: 447-455
  • DOI: 10.1097/ijg.0b013e3182894a16
  • Peer Reviewed
• [Journal Article] Differentiation by imaging of superior segmental optic hypoplasia and normal-tension glaucoma with inferior visual field defects only (2013)
  • Author(s): Yamada M, Ohkubo S, Higashide T, (他3名)
  • Journal Title: Jpn J Ophthalmol Volume: 57 Issue: 1 Pages: 25-33
  • DOI: 10.1007/s10384-012-0200-1
  • NAID: 10031168351
  • Peer Reviewed
• [Journal Article] Relationship between optic disc ovality and horizontal disc tilt in normal young subjects (2013)
  • Author(s): Takasaki H, Higashide T, (他3名)
  • Journal Title: Jpn J Ophthalmol Volume: 57 Issue: 1 Pages: 34-40
  • DOI: 10.1007/s10384-012-0193-9
  • NAID: 10031168352
  • Peer Reviewed
• [Journal Article] Relationship between macular ganglion cell complex parameters and visual field parameters after tumor resection in chiasmal compression (2012)
  • Author(s): Ohkubo S, Higashide T, (他4名)
  • Journal Title: Jpn J Ophthalmol Volume: 56 Issue: 1 Pages: 68-75
  • DOI: 10.1007/s10384-011-0093-4
  • NAID: 10030248441
  • Peer Reviewed
• [Presentation] Effects of tafluprost on endothelin-1-induced retinal injury in the rat (2013)
  • Author(s): Nagata A, Oomachi K, Higashide T, (他5名)
  • Organizer: ARVO 2013 Annual Meeting
  • Place of Presentation: Seattle, USA
  • Year and Date: 2013-05-05
• [Presentation] ラットエンドセリン硝子体内投与モデルにおけるタフルプロストの神経保護効果の検討 (2012)
  • Author(s): 長田敦,大町勝美,東出朋巳,(他5名)
  • Organizer: 第23回日本緑内障学会
  • Place of Presentation: 金沢市
  • Year and Date: 2012-09-28
• [Presentation] モデル動物評価法:小動物の生体眼底観察 (2012)
  • Author(s): 東出朋巳
  • Organizer: 第32回比較眼科学会年次大会
  • Place of Presentation: 名古屋市(教育講演)
  • Year and Date: 2012-07-21
• [Presentation] モデル動物評価法:小動物の生体眼底観察 (2012)
  • Author(s): 東出朋巳
  • Organizer: 第116回日本眼科学会総会
  • Place of Presentation: 東京都(教育セミナー)
  • Year and Date: 2012-04-06
• [Presentation] ラットエンドセリン硝子体内投与による網膜各層の障害と網膜神経節細胞数との関連 (2011)
  • Author(s): 長田敦,大町勝美,東出朋巳,(他5名)
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 東京都
  • Year and Date: 2011-05-13
• [Presentation] In-vivo evaluation of thickness changes of retinal layers and retinal ganglion cells after intravitreal injection of endothelin-1 in rat eye (2011)
  • Author(s): Nagata A,Omachi K,Higashide T, (他5名
  • Organizer: World Glaucoma Congress
  • Place of Presentation: Paris,France
• [Presentation] ラットエンドセリン硝子体内投与モデルにおけるタフルプロストの神経保護効果の検討
  • Author(s): 長田 敦、大町勝美、東出朋巳ほか
  • Organizer: 第23回日本緑内障学会
  • Place of Presentation: 石川県立音楽堂（石川県）