Roles of a cell membrane-nuclear shuttle molecule Hic-5 in skin wound healing
| Project/Area Number |
23592647
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Osaka University |
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Principal Investigator |
INUI Shigeki 大阪大学, 医学(系)研究科(研究院), 寄附講座准教授 (30324750)
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| Co-Investigator(Kenkyū-buntansha) |
ITAMI Satoshi 大阪大学, 大学院医学系研究科, 寄附講座教授 (30136791)
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| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 創傷治癒学 / メラノサイト / Hic-5 / 核内因子 / 増殖 / 運動 / 浸潤 / 細胞増殖因子 / 線維芽細胞 / ケラチノサイト / 創傷治癒 / 表皮 / 皮膚再生 / 分化 |
|---|
| Research Abstract |
The immunocytochemistry revealed that Hic-5 shifts from peri-nuclei to cell membrane during keratinocyte differentiation. Using a stable knockdown HaCat keratinocytes (Hic-5KD-HaCat) and control HaCat (cHaCat), in vitro assays revealed that Hic-5 knockdown suppresses DNA synthesis and accelerates migration more than cHaCat. During HaCat keratinocyte differentiation, keratin 1 expression is suppressed in Hic-5KD-HaCat. Regarding adhesion ability to type IV collagen-coated plates, it was suppressed by Hic-5 knockdown. From these results, Hic-5 plays multipotential functions in growth, differentiation, migration and adhesion of human keratinocytes in wound healing. The 16-F1 murine melanoma cells as a model cell line for normal melanocytes were studied likewise, reveling the potential roles of Hic-5 in the proliferation and motility, suggesting its roles in re-pigmentation during wound healing.
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Report
(4 results)
Research Products
(70 results)
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[Journal Article] A practical estimation method for analyzing adverse drug reactions using data mining.2013
Author(s)
Shirakuni Y, Okamoto K, Ueshima E, Inui S, Takahara J, Ohgaru T, Yamasaki H, Tian Y, Kawashita N, Inoue R, Yasunaga T, Takagi T
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Journal Title
Drug Inf J
Volume: 47
Pages: 235-241
Related Report
Peer Reviewed
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[Journal Article] Induction of transforming growth factor-beta 1 by androgen is mediated by reactive oxygen species in hair follicle dermal papilla cells.2013
Author(s)
Shin H, Yoo HG, Inui S, Itami S, Kim IG, Cho AR, Lee DH, Park WS, Kwon O, Cho KH, Won CH
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Journal Title
BMB Rep
Volume: 46
Pages: 460-464
Related Report
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