Serum calcium upregulates Fgf23 expression by Dickopf-1-mediated inhibition of Wnt/beta-catenin signaling in bone

• Project/Area Number: 23592696
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Mukogawa Women's University (2012-2013); Tokyo Medical and Dental University (2011)
• Principal Investigator: TAMAMURA Yoshihiro 武庫川女子大学, 健康・スポーツ科学部, 博士研究員 (70431963)
• Co-Investigator(Renkei-kenkyūsha): YAMAGUCHI Akira 東京医科歯科大学, 口腔病理学講座, 教授 (00142430) ; IIMURA Tadahiro 愛媛大学, プロテオサイエンスセンターバイオイメージング部門, 准教授 (20282775) ; SAKAMOTO Kei 東京医科歯科大学, 口腔病理学講座, 助教 (00302886)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: Fgf23 / Wnt / カルシウム

Research Abstract

Fgf23 is a phosphaturic factor which is secreted from osteocytes in bone. Fgf23 plays a central role for phosphate metabolism as demonstrated by elevated serum levels of Fgf23 in hypophosphatemic diseases. Post-transcriptional modification of Fgf23 is extensively studied, however mechanisms of transcriptional regulation of Fgf23 are not fully understood. In this study, we demonstrated that serum calcium regulates Fgf23 expression by Dickopf-1-mediated inhibition of Wnt/beta-catenin signaling in bone. This result will provide the possibilities of a new therapy for hypophosphatemic diseases by using anti-Dickpof-1 antibody.

Research Products

• [Journal Article] CCN3 protein participates in bone regeneration as an inhibitory factor (2013)
  • Author(s): Matsusita Y, Sakamoto K, Tamamura Y, Shibata Y, Minamizato T, Kihara T, Ito M, Katsube K, Hiraoka S, Koseki H, Harada K, Yamaguchi A
  • Journal Title: J. Biol. Chem. Volume: 288(27) Pages: 19973-19985
  • Peer Reviewed
• [Journal Article] Spatiotemporal disorder in the axial skeleton development of the Mesp2-null mouse : a model of spondylocostal disostosis and spondylothoracic dysostosis (2013)
  • Author(s): Makino Y, Takahashi Y, Tanabe R, Tamamura Y, Watanabe T, Haraikawa M, Hamagaki M, Hata K, Kanno J, Yoneda T, Saga Y, Goseki-Sone M, Kaneko K, Yamaguchi A, Iimura T
  • Journal Title: Bone Volume: 53(1) Pages: 248-258
  • Peer Reviewed
• [Journal Article] CCN3 protein participates in bone regeneration as an inhibitory factor. (2013)
  • Author(s): Matsushita Y, Sakamoto K, Tamamura Y, Shibata Y, Minamizato T, Kihara T, Ito M, Katsube K, Hiraoka S, Koseki H, Harada K, Yamaguchi A.
  • Journal Title: The Journal of Biological Chemistry Volume: 288 Issue: 27 Pages: 19973-19985
  • DOI: 10.1074/jbc.m113.454652
  • Peer Reviewed
• [Journal Article] Spatiotemporal disorder in endochondral ossification during axial skeleton development in the Mesp2-null mouse: A developmental etiology of spondylocostal dysostosis and spondylothoracic dysostosis. (2013)
  • Author(s): Makino Y, Takahashi Y, Tanabe R, Tamamura Y, Watanabe T, Haraikawa M, Hamagaki M, Hata K, Kanno J, Yoneda T, Saga Y, Goseki-Sone M, Kaneko K, Yamaguchi A, Iimura T
  • Journal Title: BONE Volume: 53 Issue: 1 Pages: 248-258
  • DOI: 10.1016/j.bone.2012.11.033
  • Peer Reviewed
• [Journal Article] Increasing participation of sclerostin in postnatal bone development, revealed by three-dimensional immunofluorescence morphometry (2012)
  • Author(s): Watanabe T, Tamamura Y, Hoshino A, Makino Y, Kamioka H, Amagasa T, Yamaguchi A, Iimura T
  • Journal Title: Bone Volume: 51(3) Pages: 447-458
  • Peer Reviewed
• [Journal Article] Bone and tooth in calcium and phosphate metabolism (2012)
  • Author(s): Tamamura Y, Yamaguchi A
  • Journal Title: Clin. Calcium Volume: 22(1) Pages: 11-17
• [Journal Article] Bone and tooth in calcium and phosphate metabolism (2012)
  • Author(s): Tamamura, Y
  • Journal Title: Clin. Calcium Volume: 22 Pages: 11-17
• [Journal Article] Comparative morphology of the osteocyte lacunocanalicular system in various vertebrates (2011)
  • Author(s): Cao L, Moriishi T, Miyazaki T, Iimura T, Hamagaki M, Nakane A, Tamamura Y, Komori T, Yamaguchi A
  • Journal Title: J. Bone Miner. Metab. Volume: 29(6) Pages: 662-670
  • NAID: 10030895041
  • Peer Reviewed
• [Journal Article] Comparative morphology of the osteocyte lacunocanalicular system in various vertebrates (2011)
  • Author(s): Cao, L
  • Journal Title: J. Bone Miner. Metab. Volume: 29 Pages: 662-670
  • Peer Reviewed