Analysis of virulence regulatory mechanism of group A streptococcal ncRNA
| Project/Area Number |
23592700
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
NAKATA Masanobu 大阪大学, 歯学研究科(研究院), 准教授 (90444497)
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| Co-Investigator(Kenkyū-buntansha) |
KAWABATA Shigetada 大阪大学, 歯学研究科, 教授 (50273694)
TERAO Yutaka 新潟大学, 医歯学系, 教授 (50397717)
SUMITOMO Tomoko 大阪大学, 歯学研究科, 助教 (50423421)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | A群レンサ球菌 / ncRNA / 細菌 / レンサ球菌 / non-coding RNA |
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| Research Abstract |
Streptococcus pyogenes regulates expression of virulence factors in infection sites, and causes purulent diseases. Recently, transcriptional and translational regulations by RNA have been noted. In this study, non-coding RNA candidates were searched in Streptococcus pyogenes, and target mRNAs of non-coding RNAs were investigated using constructed mutant strains. Moreover, regarding the essential gene encoding a transcriptional regulator, an riboswitch was introduced upstream of the gene, and the function of the essential gene was analysed. When the translation rate of the gene was reduced in the mutant strain, the growth was markedly inhibited. Moreover, the mutant showed an abnormal cellular morphology and irregular superficial structure. Our results indicate that riboswitch-based translational control is useful for analysis of essential factors of Streptococcus pyogens.
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Report
(4 results)
Research Products
(75 results)
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[Journal Article] Assembly mechanism of FCT region type 1 pili in serotype M6 Streptococcus pyogenes2011
Author(s)
Nakata M, Kimura KR, Sumitomo T, Wada S, Sugauchi A, Oiki E, Higashino M, Kreikemeyer B, Podbielski A, Okahashi N, Hamada S, Isoda R, Terao Y, and Kawabata S
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Journal Title
J Biol Chem
Volume: 286
Issue: 43
Pages: 37566-37577
DOI
Related Report
Peer Reviewed
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