Elucidation of the pathological mechanism on abnormal iron metabolism using a rat model

• Project/Area Number: 23592735
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: NOMA Takafumi 徳島大学, ヘルスバイオサイエンス研究部, 教授 (40189428)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: Sp6 / 歯原性上皮細胞 / 鉄代謝 / テトラサイクリン遺伝子誘導系 / 細胞内輸送 / Rock1 / 切歯歯原性上皮細胞 / エナメル芽細胞分化 / 遺伝子発現調節 / 転写調節 / ヒストン修飾 / 翻訳後修飾 / プロテアソーム / iron metabolism

Research Abstract

Based on our finding that Sp6 functions in not only morphological differentiation but also iron metabolism during tooth development, I performed a study to clarify a role of iron metabolism in the developmental control of Sp6. The iron supply for the ameloblast was the same as normal control in Sp6 Tg rat, suggesting two possibilities of the release imperfection from lysosomal ferritin and iron complex in the cell, or the secretion imperfection from the ameloblasts. Then, to further analyze the relationship between Sp6 and lysosomal function or secreting mechanism, I tried to condtruct the tet induction system for Sp6 using the dental epithelial cells that were originally established in my laboratory. Unfortunately, I could not lead to establish the tet inducible system by this moment. In addition, I found the involvement of Sp6 in Rock1 gene regulation, which is related to secretion mechanism.

Research Products

• [Journal Article] Sp6 regulation of Rock1 promoter activity in dental epithelial cells (2014)
  • Author(s): Yanuaryska RD, Miyoshi K, Adiningrat A, Horiguchi T, Tanimura A, Hagita H, and Noma T.
  • Journal Title: J Med Invest Volume: (in press)
  • NAID: 130004822736
  • Peer Reviewed
• [Journal Article] Ctip2-mediated Sp6 transcriptional regulation in dental epithelium-derived cells (2014)
  • Author(s): Adiningrat A, Tanimura A, Miyoshi K, Yanuaryska RD, Hagita H, Horiguchi T, and Noma T
  • Journal Title: J Med Invest Volume: 61 Pages: 126-136
  • NAID: 130004822699
  • Peer Reviewed
• [Journal Article] Sp6 regulation of Rock1 promoter activity in dental epithelial cells (2014)
  • Author(s): Ryna Dwi Yanuaryska, Keiko Miyoshi, Arya Adiningrat, Taigo Horiguchi, Ayako Tanimura, Hiroko Hagita and Takafumi Noma
  • Journal Title: The Journal of Medical Investigation Volume: ―
  • NAID: 130004822736
  • Peer Reviewed
• [Journal Article] Ctip2-mediated Sp6 transcriptional regulation in dental epithelium-derived cells (2014)
  • Author(s): Arya Adiningrat, Ayako Tanimura, Keiko Miyoshi, Ryna Dwi Yanuaryska, Hiroko Hagita, Taigo Horiguchi, and Takafumi Noma
  • Journal Title: The Journal of Medical Investigation Volume: 61 Pages: 126-136
  • NAID: 130004822699
  • Peer Reviewed
• [Journal Article] Dynamic Changes of Sp6 Transgene Expression in Dental Epithelial Cells during Long-term Culture. (2013)
  • Author(s): Utami TW et al
  • Journal Title: Indonesian Journal of Dental Research Volume: in press
  • Peer Reviewed
• [Journal Article] Novel genetic linkage of rat Sp6 mutation to Amelogenesis imperfecta. (2012)
  • Author(s): Muto T, Miyoshi K et al
  • Journal Title: Orphanet Journal of Rare Diseases Volume: Jun 7 Issue: 1 Pages: 34-44
  • DOI: 10.1186/1750-1172-7-34
  • Peer Reviewed
• [Journal Article] Dissection of morphological and metabolic differentiation of ameloblasts via ectopic SP6 expression (2012)
  • Author(s): Muto T, Miyoshi K, Horiguchi T
  • Journal Title: J Med Invest Volume: 59 Pages: 59-68
  • Peer Reviewed
• [Journal Article] Dissection of morphological and metabolic differentiation of ameloblasts via ectopic SP6 expression. (2012)
  • Author(s): Muto T, Miyoshi K et al
  • Journal Title: J Med Invest. Volume: 59(1-2) Pages: 59-68
  • Peer Reviewed
• [Journal Article] Dissection of morphological and metabolic differentiation of ameloblasts via ectopic SP6 expression (2012)
  • Author(s): Muto T,et al.
  • Journal Title: J Med Investigation Volume: 59 Pages: 59-68
  • Peer Reviewed
• [Journal Article] iPS細胞を用いた臨床応用の基礎と課題 (2012)
  • Author(s): 野間隆文
  • Journal Title: 四国歯学会雑誌 Volume: 24 Pages: 59-68
  • NAID: 120005832463
• [Journal Article] Possible linkage of SP6 transcriptional activity with amelogenesis by protein stabilization (2011)
  • Author(s): Utami TW, Miyoshi K, Hagita H, Yanuaryska RD, Horiguchi T, Noma T
  • Journal Title: J Biomed Biotech Volume: ID320987 Issue: 1 Pages: 1-10
  • DOI: 10.1155/2011/320987
  • NAID: 120006783225
  • Peer Reviewed
• [Presentation] Role of Ctip2 in Sp6 gene regulation (2013)
  • Author(s): Adiningrat A, Tanimura A, Miyoshi K, Yanuaryska RD, Horiguchi T, Hagita H, and Noma T
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2013-12-04
• [Presentation] SP6 Positively Regulates Rock1 Promoter Activity in Dental Epithelial Cells (2013)
  • Author(s): Yanuaryska RD, Miyoshi K, Horiguchi T, Tanimura A, Adiningrat A, and Noma T
  • Organizer: 第55回歯科基礎医学会
  • Place of Presentation: 岡山コンベンションセンター(岡山県)
  • Year and Date: 2013-09-21
• [Presentation] 転写因子SP6の構造と機能解析 (2013)
  • Author(s): 三好圭子, Yanuaryska RD, Adiningrat A,萩田浩子,谷村綾子,堀口大吾
  • Organizer: 第54回日本生化学会中国・四国支部例会
  • Place of Presentation: 徳島大学大塚講堂(徳島県)
  • Year and Date: 2013-06-01
• [Presentation] SP6 and AP2 regulate Rock1 expression in dental epithelial cells (2013)
  • Author(s): Yanuaryska RD, Miyoshi K, Horiguchi T, Tanimura A, Hagita H, and Noma T
  • Organizer: 第54回日本生化学会中国・四国支部例会
  • Place of Presentation: 徳島大学大塚講堂(徳島県)
  • Year and Date: 2013-06-01
• [Presentation] Role of Ctip2 in Sp6 gene regulation (2013)
  • Author(s): Adiningrat Arya, Ayako Tanimura, Keiko Miyoshi, Yanuaryska Dwi Ryna, Taigo Horiguchi, 萩田 浩子 and Takafumi Noma
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
• [Presentation] SP6 Positiyvely Regulates Rock1 Promoter Activity in Dental Epithelial Cells (2013)
  • Author(s): Yanuaryska Dwi Ryna, Keiko Miyoshi, Taigo Horiguchi, Ayako Tanimura, Adiningrat Arya and Takafumi Noma
  • Organizer: 第55回歯科基礎医学会
  • Place of Presentation: 岡山コンベンションセンター（岡山県岡山市）
• [Presentation] 転写因子SP6の構造と機能解析 (2013)
  • Author(s): 三好 圭子, Yanuaryska Dwi Ryna, Adiningrat Arya, 萩田 浩子, 谷村 綾子, 堀口 大吾, 野間 隆文
  • Organizer: 第54回 日本生化学会 中国・四国支部例会
  • Place of Presentation: 徳島大学大塚講堂（徳島県徳島市）
• [Presentation] P6 and AP2 regulate Rock1 expression in dental epithelial cells (2013)
  • Author(s): Ryna Dwi Yanuaryska, Keiko Miyoshi, Taigo Horiguchi, Ayako Tanimura, Hiroko Hagita and Takafumi Noma
  • Organizer: 第54回 日本生化学会 中国・四国支部例会
  • Place of Presentation: 徳島大学大塚講堂（徳島県徳島市）
• [Presentation] エナメル質形成不全症の新規遺伝子の発見 (2012)
  • Author(s): 三好圭子,武藤太郎,堀口大吾、萩田浩子、野間隆文
  • Organizer: 第57回日本人類遺伝学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2012-10-25
• [Presentation] Role of Bcl11b/Ctip2 on Sp6 gene expression in dental epithelial cells. (2012)
  • Author(s): Arya Adiningrat, et al
  • Organizer: ASEAN plus and TOKUSHIMA Joint International Conference
  • Place of Presentation: Royal Ambarukmo Hotel(Indonesia)
• [Presentation] Sp6 regulation of Rock1 expression indental epithelial cells. (2012)
  • Author(s): Ryna Dwi Yanuariska, et al
  • Organizer: ASEAN plus and TOKUSHIMA Joint International Conference.
  • Place of Presentation: Royal Ambarukmo Hotel(Indonesia)
• [Presentation] A frameshift mutation in Sp6 linked to Amelogenesis imperfect. (2012)
  • Author(s): Miyoshi K, Muto T, et al
  • Organizer: TOKUSHIMA Joint International Conference.
  • Place of Presentation: Royal Ambarukmo Hotel(Indonesia)
• [Presentation] Regulation of Sp6 gene expression and cell-type specific function in dental epithelial cells (2012)
  • Author(s): Utami TW
  • Organizer: The 2nd international Joint Sympojiumu on Oral and Dental Sciences in conjugation with Dental Specialists Seminar
  • Place of Presentation: Inna Garuda Hotel(Indonesia yogyakarta)
• [Presentation] Identification of SP6 target genes in rat dental epithelial cell (2011)
  • Author(s): Yanuaryska RD, Miyoshi K, Utami TW, Horiguchi T, and Noma T
  • Organizer: 第53回歯科基礎医学会
  • Place of Presentation: 長良川国際会議場(岐阜県)
  • Year and Date: 2011-10-01
• [Presentation] SP6 stability and functional linkage during amelogenesis (2011)
  • Author(s): Utami TW, Miyoshi K, Hagita H, Yanuaryska RD, Horiguchi T, and Noma T
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-09-22
• [Presentation] Regulation of Sp6 expression and function in C9 cells (2011)
  • Author(s): Utami TW
  • Organizer: 第52回日本生化学会中国四国支部総会
  • Place of Presentation: 広島大学広仁会館（広島市）
• [Presentation] Regulation of Sp6 expression and function in dental epithelial cells (2011)
  • Author(s): Utami TW
  • Organizer: 第５回日本エピジェネティック研究会
  • Place of Presentation: ＫＫＲホテル熊本（熊本市）
• [Presentation] Sp6トランスジェニックラットに見られた鉄代謝異常 (2011)
  • Author(s): 野間隆文
  • Organizer: 第35回日本鉄バイオサイエンス学会
  • Place of Presentation: 徳島文理大学国際会議場（徳島市）
• [Presentation] SP6 stability and functional linkage during amelogenesis (2011)
  • Author(s): Utami TW
  • Organizer: 第84回日本生化学大会
  • Place of Presentation: 国立京都国際会館（京都市）
• [Presentation] Identification of SP6 target genes in rat ental epithelial cells (2011)
  • Author(s): Ryna Dwi yanuaryska
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 長良川国際会議場（岐阜市）