| Project/Area Number |
23592754
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
INOUYE Satoshi JNC(株), 横浜研究所, 主席研究員 (40426622)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 生物発光イメージング / MMP-2 / 開口分泌 / タンパク質分泌 / がん細胞 / 遊走細胞 / bioluminescence imaging / Gaussia luciferase / exocytosis / coelenterazine / Bioluminescence imaging / cell migration / bioluminescence |
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| Research Abstract |
MMP-2 is one of the enzymes in degradation of basement membrane collagen and has a major role in cancer cell invasion. MMP-2 is secreted as the inactive pro-form, and the inactive pro-form of MMP-2 binds on the cell surface, and then active MMP-2 is produced. We have established a method of video-rate bioluminescence imaging to visualize exocytotic protein secretion from a single living cell. This bioluminescence method has advantages for estimation of time-dependent changes of the amount of secreted protein and or visualization of protein secretion over the whole surface of a cell. As a result, we demonstrated that the repeated secretion of MMP-2 from specific sites at the leading edge of the cell. The analyses of bioluminescence video images of MMP2-GLase showed repeated secretion of MMP-2 from specific sites at the leading edge and bottom side of the cell, suggesting that the sites of MMP-2 secretion differ from the MMP-2 binding sites.
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