Attempts to identify the hard tissue regeneration by human dental pulp stem cells

• Project/Area Number: 23592806
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Meikai University
• Principal Investigator: KATAYAMA Tadashi 明海大学, 歯学部, 教授 (10105596)
• Co-Investigator(Kenkyū-buntansha): SAKAGAMI Hiroshi 明海大学, 歯学部, 教授 (50138484) ; UMEMURA Naoki 明海大学, 歯学部, 助教 (80609107) ; ISHIHARA Sachiyo 明海大学, 歯学部, 講師 (10223017) ; 岩坂 憲助 明海大学, 歯学部, 講師 (60424058)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 再生医学 / 歯学 / 生体材料 / 歯髄 / 幹細胞 / 歯髄幹細胞 / 硬組織再生 / 多血小板血漿

Research Abstract

In general, undifferentiated mesenchymal cells are in abundant supply in human dental pulp tissue, and it is known that containing a large number of stem cells. However, the clarification of characteristics of dental pulp stem cells (DPSCs) and differentiation signaling are still unknown. Therefore, We determined expression of CD44 that is known as a stem cell surface marker upon human DPSCs. The CD44 expression was approximately 60%. Then, hyaluronic acid that is a ligand of CD44 treatment induced expression of alkaline phosphatase. These data suggested that CD44 signaling may induce efficient calcification on DPSCs.

Research Products

• [Journal Article] 3,5-bis{4-[4-(2-Aminoethoxy)phenylcarbo nyloxy]benzylidene}-1-methyl-4-piperido nes : A novel class of tumour-selective cytotoxins (2014)
  • Author(s): Alaa Baryyan, Umashankar Das, Hiroshi Sakagami, Tadashi Katayama, Jan Balzarini, Erik De Clercq, Masami Kawase Joseph Molnár, Rajendra K. Sharma, and Jonathan R.Dimmock
  • Journal Title: J Enzyme Inhib Med Chem Volume: (in press)
• [Journal Article] CD44 expression in human head and neck squamous cell carcinoma (HNSCC)is stimulated by baicalin, a common constituent of traditional herbal (Kampo)medicines classified as Bupleurum root drugs, and its aglycone baicalein (2014)
  • Author(s): Emika Ohkoshi, Naoki Umemura, Tadashi Katayama, Hiroshi Sakagami
  • Journal Title: Food and Chemical Toxicology Volume: (in press)