Purification, structure characterization of bioactive substances from marine organisms and application them to the novel targeting oral cancer drugs

• Project/Area Number: 23592962
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: YUKIO YOSHIOKA 広島大学, 医歯薬保健学研究院, 助教 (20335665)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Tetsuji 広島大学, 医歯薬保健学研究院, 教授 (00169153) ; OJIKA Makoto 名古屋大学, 生命農学研究科, 教授 (50152492) ; HAYASHIDO Yasutaka 広島大学, 病院, 講師 (70243251) ; SHINTANI Tomoaki 広島大学, 病院, 助教 (90403518)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 海洋生物 / 生理活性物質 / 抗腫瘍効果 / 口腔がん / 癌幹細胞 / 分子標的薬 / 口腔癌 / 口腔癌幹細胞 / 分子標的治療薬 / がん幹細胞

Research Abstract

Marine organisms from Japanese adjacent Sea is collected and freeze-dried. They are homogenized in EtOH-MeOH(4:1)and distributed by HPLC into hexane layer and C2H5OCH3CO layer and aqueous layer. Following it, the extracted compounds are investigated their activity by bio-assay and are purified and decided their structure by LC/MS. Their analogues improving the structure of their branches are produced and massively synthesized them. The anti-tumor activity is investigated by the inoculated tumor cell on nude mouse. CD133 is a useful marker of the oral squamous cell carcinoma cells. The oral cancer stem cells were isolated from the oral squamous cell carcinoma cell line with index of CD133 and analyzed the character of a stem cell. The mechanism of the novel anti-tumor agents against not only the oral cancer but also the oral cancer stem cells is analyzed in vitro.

Research Products

• [Journal Article] Petrosiols A-E, neurotrophic diyene tetraols from the Okinawan sponge Petrosia strongylata (2013)
  • Author(s): Kouhei Horikawa, Takuya Yagyu, Yukio Yoshioka, Takeshi Fujiwara, Akihiko Kanamoto, Tetsuji Okamoto, Makoto Ojika
  • Journal Title: Tetrahedron Volume: 69 Pages: 101-106
  • Peer Reviewed
• [Journal Article] Ectomesenchymal chondromyxoid tumor of the tongue: insights on histogenesis. (2013)
  • Author(s): Yoshioka Y, , Toratani S, Okamoto T. 他3名
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol. Volume: Feb;115(2) Issue: 2 Pages: 233-240
  • DOI: 10.1016/j.oooo.2012.11.008
  • Peer Reviewed
• [Journal Article] Ameloblastic carcinoma, secondary type, of the mandible: a case report. (2013)
  • Author(s): Yoshioka Y, Toratani S, Ogawa I, Okamoto T.
  • Journal Title: J Oral Maxillofac Surg. Volume: Jan;71(1) Issue: 1 Pages: 58-62
  • DOI: 10.1016/j.joms.2012.09.005
  • Peer Reviewed
• [Journal Article] 多科連携により初期治療と欠損部の再建をなし得た重症口腔顎顔面外傷の一例 (2013)
  • Author(s): 吉岡幸男,虎谷茂昭,伊藤翼,山崎佐知子,藤井良典,小泉浩一,久保隆靖,林堂安貴,岡本哲治
  • Journal Title: 日本口腔顎顔面外傷学会雑誌 Volume: 12(1) Pages: 37-43
  • Peer Reviewed
• [Journal Article] Ectomesenchymal chondromyxoid tumor of the tongue: insights on histogenesis. (2013)
  • Author(s): Yoshioka Y, Ogawa I, Tsunematsu T, Sakaue T, Yamasaki S, Fukui Y, Hayashido Y, Toratani S, Okamoto T.
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol. Volume: 115 Pages: 230-240
  • Peer Reviewed
• [Journal Article] 多科連携により初期治療と欠損部の再建をなし得た重症口腔顎顔面外傷の一例 (2013)
  • Author(s): 吉岡幸男 虎谷茂昭 伊藤翼 山崎佐知子 藤井良典 小泉浩一 久保隆靖 林堂安貴 岡本哲治
  • Journal Title: 日本口腔顎顔面外傷学会雑誌 Volume: 12 Pages: 37-43
  • Peer Reviewed
• [Journal Article] Petrosiols A-E, neurotrophic diyne tetraols isolated from the Okinawan sponge Petrosia strongylata (2013)
  • Author(s): Kouhei horikawa,Takuya Yagyu, Yukio Yoshioka, Takeshi Fujiwara, Akihiko Kanamoto, Tetsuji Okamoto, Makoto Ojika
  • Journal Title: tetrahedron Volume: 69 Pages: 101-106
  • Peer Reviewed
• [Journal Article] Ameloblastic carcinoma, secondary type, of the mandible: a case report (2013)
  • Author(s): Yoshioka Y, Toratani S, Ogawa I, Okamoto T,
  • Journal Title: J. Oral Maxillofac Surg. Volume: 71(1)
  • Peer Reviewed
• [Journal Article] Ectomesenchymal chondromyxoid tumor of the tongue: insights on histogenesis (2013)
  • Author(s): Yoshioka Y, Ogawa I, Tsunematsu T, Sakaue T, Yamasaki S, Fukui Y, Hayashido Y, Toratani S, Okamoto T,
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol. Volume: 115(2) Pages: 233-40
  • Peer Reviewed
• [Journal Article] ビスフォスフォネート関連顎骨壊死にて腐骨除去術を行い長期観察し得た骨Paget's病の１例 (2012)
  • Author(s): 吉岡幸男 虎谷茂昭 向笠英恵 神田拓 新谷智章 岡本哲治
  • Journal Title: 日本口腔科学会雑誌 Volume: 61 Pages: 59-65
  • Peer Reviewed
• [Journal Article] Cyclolobatriene, a novel prenylated germacrene diterpene, from the soft coral Lobophytum pauciflorum. (2012)
  • Author(s): Govindam SV, Yoshioka Y, Kanamoto A, Fujiwara T, Okamoto T, Ojika M.
  • Journal Title: Bioorg Med Chem. Volume: 15;20(2) Issue: 2 Pages: 2687-692
  • DOI: 10.1016/j.bmc.2011.12.012
  • Peer Reviewed
• [Journal Article] ビスフォスフォネート関連顎骨壊死にて腐骨除去術を行い長期観察し得た骨Paget病の１例 (2012)
  • Author(s): 吉岡幸男 虎谷茂昭 向笠英恵 神田拓 新谷智章 岡本哲治
  • Journal Title: 日本口腔科学会雑誌 Volume: 61 Pages: 59-65
  • NAID: 10030795759
  • Peer Reviewed
• [Presentation] 当科における唾液腺腫瘍の臨床的検討鍋島巧 (2013)
  • Author(s): 濱田充子,吉岡幸男,小泉浩一,谷亮治,林堂安貴,虎谷茂昭,岡本哲治
  • Organizer: 第58回(公社)日本口腔外科学会総会・学術大会
  • Place of Presentation: 福岡
• [Presentation] 当科における唾液腺腫瘍の臨床的検討 (2013)
  • Author(s): 鍋島功，濱田充子，吉岡幸男，小泉浩一，谷亮治，林堂安貴，虎谷茂昭，岡本哲治
  • Organizer: 日本口腔外科学会総会・学術大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] Ectomesenchymal chondromyxoid tumorの1例 (2012)
  • Author(s): 坂上泰士,吉岡幸男,山崎佐知子,小川郁子,林堂安貴,虎谷茂昭,岡本哲治
  • Organizer: 第56回(社)日本口腔外科学会総会・学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-10-21
• [Presentation] 上唇粘膜下に生じた筋上皮腫の一例 (2012)
  • Author(s): 木村直大,吉岡幸男,小泉浩一,小川郁子,虎谷茂昭,岡本哲治
  • Organizer: 第40回(社)日本口腔外科学会中・四国地方会
  • Place of Presentation: 島根
  • Year and Date: 2012-05-28
• [Presentation] 左側顎下部にみられた石灰化リンパ節の1例 (2012)
  • Author(s): 藤井良典,吉岡幸男,新谷智章,常松貴明,小川郁子,虎谷茂昭,岡本哲治
  • Organizer: 第40回(社)日本口腔外科学会中・四国地方会
  • Place of Presentation: 島根
  • Year and Date: 2012-05-28
• [Presentation] 口腔内に生じたMALTリンパ腫の3例 (2012)
  • Author(s): 赤木恵理,吉岡幸男,岡本康正,小泉浩一,小川郁子,林堂安貴,虎谷茂昭,岡本哲治
  • Organizer: 第41回(社)日本口腔外科学会中・四国地方会
  • Place of Presentation: 愛媛
  • Year and Date: 2012-04-28
• [Presentation] 下顎粉砕骨折を伴った広範囲な重症顔面〜一次救命処置から欠損部再建へ〜 (2012)
  • Author(s): 吉岡幸男,虎谷茂昭,林堂安貴,伊藤奈七子,伊藤翼,久保隆靖,高本宗男,廣橋伸之,谷川攻一,岡本哲治
  • Organizer: 第66回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 広島
• [Presentation] 下顎粉砕骨折を伴った広範囲な重傷顎顔面外傷の治療の一例 (2012)
  • Author(s): 吉岡幸男，虎谷茂昭，林堂安貴，伊藤奈七子，伊藤翼，久保隆靖，高本宗男，広橋伸之，谷川功一，岡本哲治
  • Organizer: 日本口腔科学会学術集会
  • Place of Presentation: 広島国際会議場
• [Presentation] Ectomesenchymal chondromyxoid tumorの１例 (2011)
  • Author(s): 坂上泰士 吉岡幸男 山崎佐知子 小川郁子 林堂安貴 虎谷茂昭 岡本哲治
  • Organizer: 日本口腔外科学会
  • Place of Presentation: 大阪国際会議場