Purification, structure characterization of bioactive substances from marine organisms and application them to the novel targeting oral cancer drugs
| Project/Area Number |
23592962
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
YUKIO YOSHIOKA 広島大学, 医歯薬保健学研究院, 助教 (20335665)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Tetsuji 広島大学, 医歯薬保健学研究院, 教授 (00169153)
OJIKA Makoto 名古屋大学, 生命農学研究科, 教授 (50152492)
HAYASHIDO Yasutaka 広島大学, 病院, 講師 (70243251)
SHINTANI Tomoaki 広島大学, 病院, 助教 (90403518)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 海洋生物 / 生理活性物質 / 抗腫瘍効果 / 口腔がん / 癌幹細胞 / 分子標的薬 / 口腔癌 / 口腔癌幹細胞 / 分子標的治療薬 / がん幹細胞 |
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| Research Abstract |
Marine organisms from Japanese adjacent Sea is collected and freeze-dried. They are homogenized in EtOH-MeOH(4:1)and distributed by HPLC into hexane layer and C2H5OCH3CO layer and aqueous layer. Following it, the extracted compounds are investigated their activity by bio-assay and are purified and decided their structure by LC/MS. Their analogues improving the structure of their branches are produced and massively synthesized them. The anti-tumor activity is investigated by the inoculated tumor cell on nude mouse. CD133 is a useful marker of the oral squamous cell carcinoma cells. The oral cancer stem cells were isolated from the oral squamous cell carcinoma cell line with index of CD133 and analyzed the character of a stem cell. The mechanism of the novel anti-tumor agents against not only the oral cancer but also the oral cancer stem cells is analyzed in vitro.
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Report
(4 results)
Research Products
(21 results)
