Development of the new bone regeneration method by transplanting jawbone periosteum sheets as a mechanosensor.

• Project/Area Number: 23592979
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hirosaki University
• Principal Investigator: KIMURA Hiroto 弘前大学, 医学(系)研究科(研究院), 教授 (90142851)
• Co-Investigator(Kenkyū-buntansha): WATARU Kobayashi 弘前大学, 医学研究科, 准教授 (50234860) ; NAKAGAWA Hiroshi 弘前大学, 医学研究科, 助教 (00529688)
• Research Collaborator: MATSUMIYA Tomoh 弘前大学, 医学研究科, 助教 (30344592) ; KUBOTA Kousei 弘前大学, 医学部附属病院, 助教 (10529689) ; SAKAKI Hirotaka 弘前大学, 医学部附属病院, 講師 (90374850) ; ITO Ryohei 弘前大学, 医学部附属病院, 医員 (20638902)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 骨膜 / メカノセンサー / 骨代謝 / Wnt pathway / BMP pathway / メカニカルストレス / Wnt signaling / BMP signaling / 骨リモデリング / 歯学 / 移植・再生医療 / 細胞・組織 / 臨床 / 骨増生法

Research Abstract

The relationship between mechanical stress and osteogenesis has been widely studied. However, little is known about the functions of the periosteum in bone remodeling under mechanical stress. We hypothesized that the periosteum senses a mechanical stress to induce bone remodeling, therefore, we subjected periosteum cells to load mechanical stretching and characterized their gene expression profiles.
Periosteum cells appear to induce, in response to the stretch stimulus, gene expression changes in WNT pathway and BMP pathway, which cooperate to promote osteoblast differentiation and osteogenesis. Our results also suggest that during mechanical stress-induced bone remodeling in the body, the periosterum senses the mechanical stress to control signals for promoting osteoblast differentiation and osteogenesis.

Research Products

• [Journal Article] Periosteum-derived cells respond to mechanical stretch and activate Wnt andBMP signaling pathways (2014)
  • Author(s): Ito R, Matsumiya T, Kon T, Narita N, Kubota K, Sakaki H, Ozaki T, Imaizumi T, Kobayashi W, Kimura H
  • Journal Title: Biomedical Research Volume: 35 Issue: 1 Pages: 69-79
  • DOI: 10.2220/biomedres.35.69
  • NAID: 130004903861
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed
• [Journal Article] Periosteum-derived cells respond to mechanical stretch and activate Wnt and BMP signaling pathways (2014)
  • Author(s): Ryohei Ito, Tomoh Matsumiya, Takao Kon, Norihiko Narita, Kosei Kubota, Hirotaka Sakaki, Hidemi Yoshida, Tadaatsu Imaizumi, Wataru Kobayashi, Hiroto Kimura
  • Journal Title: Biomedical Reserch Volume: vol.35 Pages: 69-79
  • Peer Reviewed
• [Presentation] 骨膜由来細胞はメカニカルストレスに応答しWnt signaling pathwayとBMP signaling pathwayが活性化される (2014)
  • Author(s): 伊藤良平、中川祥、小林恒、木村博人、他
  • Organizer: 日本口腔科学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-05-09
• [Presentation] 骨膜由来細胞はメカニカルストレスに応答しWnt signaling pathwayと BMP signaling pathwayが活性化される (2014)
  • Author(s): 伊藤 良平、佐藤 寿、榊 宏剛、久保田 耕世、今 敬生、小林 恒、木村 博人
  • Organizer: 日本口腔科学会
  • Place of Presentation: 東京都新宿区
• [Presentation] Usefulness of two-stage surgery for benign odontogenic tumors showing large cystic, intraosseous lesions in the mandible (2012)
  • Author(s): Hiroto KIMURA
  • Organizer: 中華民国口腔外科学会
  • Place of Presentation: 台中市
  • Year and Date: 2012-03-10
  • Invited
• [Presentation] Usefulness of two-stage surgery for benign odontogenic tumors showing large cystic, intraosseous lesions in the mandible (2012)
  • Author(s): Hiroto KIMURA
  • Organizer: 第13回中華民国口腔顎面外科学会(招待講演)
  • Place of Presentation: 中山医科大学(台中市)