| Project/Area Number |
23592982
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KENJI Izumi 新潟大学, 医歯学系, 教授 (80242436)
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| Co-Investigator(Renkei-kenkyūsha) |
TERASHI Hiroto 神戸大学, 医学部, 教授 (80217421)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 移植・再生医療 / 口腔顎顔面再建外科学 / 口腔粘膜 |
|---|
| Research Abstract |
We have investigated the application of ex vivo-produced oral mucosa equivalent (EVPOME) and its ability of oral mucosa regeneration. However, cultivated epithelial cells using the present methods has heterogeneity, consequently, there is a risk that EVPOME possess poor cell growth activity and capability of oral mucosa regeneration. The objective of the present study was to investigate the capability of EVPOME fabricated with small-sized cell population in which oral mucosal progenitor/stem-cell-enriched subpopulation present and the change after EVPOME grafting subcutaneously in mice histologically. More Ki-67 positive cells were observed in the epithelial cells of EPOME fabricated by small-sized cell population. Epithelial elongation of EVPOME fabricated by small-sized cell population occurs faster than that of EVPOME using present methods. These findings suggest that EVPOME with small-sized cell population has high activity and capability of oral mucosal regeneration.
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