Epigenetic regulation of primary lymphoid organ microenvironment

• Project/Area Number: 23616003
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: epigenetics
• Research Institution: Hiroshima University
• Principal Investigator: KANNO Masamoto 広島大学, 医歯薬保健学研究院(医), 教授 (40161393)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ポリコーム / タンパク質複合体 / KOマウス / lincRNA / 胸腺 / T細胞分化 / エピジェネティックス / リンパ球分化 / RNA / noncoding RNA / 蛋白質複合体 / 胸腺内T細胞分化 / ポリコーム蛋白質複合体 / 国際情報交流 / 英国・スイス / lncRNA / 胸腺上皮細胞

Research Abstract

We succeeded to purify thymic development stage specific PRC1 protein complexes. At ETP stage, PRC1.2 is major form of PRC1, however, at DN3 PRC1.4 form of PRC1 is major form. This corresponds well for the phenotype of different PCGF gene KO mice. We also succeeded to establish several floxed PcG gene KO mice. Also we identified new lincRNA with might bind with PRC2

Research Products

• [Journal Article] The Arf/p53 protein module, which induces apoptosis, down-regulates histone H2AX to allow normal cells to survive in the presence of anti-cancer drugs. (2013)
  • Author(s): Yuko Atsumi, Aki lnase, Eiji Sugihara, Ryo Sakasai, Hiroaki Fujimori, Hirobumi Teraoka, Hideyuki Saya, Masamoto Kanno, Fumio Tashiro, Hitoshi Nakagami, Mitsuko Masutani, Ken-ichi Yoshioka
  • Journal Title: The Journal of Biological Chemistry Volume: 288巻 Issue: 19 Pages: 13269-13277
  • DOI: 10.1074/jbc.m112.402560
  • Peer Reviewed
• [Journal Article] Ink4a and Arf are crucial factors in the determination of the cell of origin and the therapeutic sensitivity of Myc-induced mouse lymphoid tumor (2012)
  • Author(s): Sugihara E, Saya H, et al
  • Journal Title: Oncogene Volume: (印刷中) Issue: 23 Pages: 2849-2861
  • DOI: 10.1038/onc.2011.462
  • Peer Reviewed
• [Journal Article] A methyl-deficient diet modifies early B cell development (2012)
  • Author(s): T. Kurogi
  • Journal Title: Pathobiology Volume: 79 Issue: 4 Pages: 209-218
  • DOI: 10.1159/000337290
  • Peer Reviewed
• [Journal Article] Mel-18 controls the enrichment of tumor-initiating cells in SP fraction in mouse breast cancer (2011)
  • Author(s): Janakiraman H, Nobukiyo A, Inoue H, Kanno M
  • Journal Title: Hiroshima J Med Sci Volume: 60(2) Pages: 25-35
  • NAID: 120005323960
  • URL: http://ir.lib.hiroshima-u.ac.jp/metadb/up/kiyo/AA00664312/HiroshimaJMedSci_60_25.pdf
  • Peer Reviewed
• [Journal Article] Polycomb group gene Bmi1 plays a role in the growth of thymic epithelial cells (2011)
  • Author(s): Guo Y, Miyazaki M, Itoi M, Satoh R, Iwama A, Amagai T, Kawamoto H, Kanno M
  • Journal Title: Eur J Immunol Volume: 41 Issue: 4 Pages: 1098-1107
  • DOI: 10.1002/eji.201040794
  • Peer Reviewed
• [Presentation] Heterogeneity of Polycomb Repressive Complexes (PRCs) and long-noncoding RNA dictate the regulation network of intra-thymic T cell development (2014)
  • Author(s): Masamoto Kanno, Yun Guo, Yoshinori Ohno and Yoshihiro Takihara
  • Organizer: 4th International Symposium of RIRBM
  • Place of Presentation: Hiroshima/JAPAN
• [Presentation] Heterogeneity of Polycomb repressive complexes and long-noncoding RNA dictate the regulation network of thymic T cell development (2013)
  • Author(s): M. Kanno, Y. Guo, W.S. Kong, A. Nobukiyo, H. Inoue
  • Organizer: 15^<th> International congress of Immunology
  • Place of Presentation: Milan/Italy
• [Presentation] Polycomb Connection between Coronary Artery Disease Risk and Irradiation-Regeneration process of Thymus via Long-noncoding RNA (2013)
  • Author(s): M.Kanno, A.Nobukiyo, Y.Ohno, Y.Takihara, Y.Guo
  • Organizer: 3rd International Symposium of RIRBM
  • Place of Presentation: Hiroshima University(Hiroshima Japan)
• [Presentation] Heterogeneity of Polycomb repressive complexes and long-noncoding RNA dictate the regulation network of thymic T cell development (2013)
  • Author(s): Masamoto Kanno, Yun Guo, Kong Weng Sheng, Asako Nobukiyo, and Hiroko Inoue
  • Organizer: 15th International Congress of Immunology 2013
  • Place of Presentation: Milan/ITALY
• [Presentation] Arfとp53に依存的なヒストンH2AXの低下と静止状態 (2012)
  • Author(s): 大澤智之, 熱海悠子, 杉原英志, 佐谷秀行, 菅野雅元, 田代文夫, 益谷美都子, 吉岡研一
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 抗癌剤存在下での優先的な癌細胞死と正常細胞の生存に資するArf/p53依存的H2AX制御機構 (2012)
  • Author(s): 熱海悠子, 稲瀬安希, 杉原英志, 佐谷秀行, 菅野雅元, 益谷美都子, 吉岡研一
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] UVに応答した核内のIKKbetaを介したNF-kappaB活性化と細胞死 (2012)
  • Author(s): 土谷佳弘, 金本麻裕, 浅野知一郎, 菅野雅元, 鎌田英明
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Arfとp53に依存的なヒストンH2AXの低下と静止状態 (2012)
  • Author(s): 大澤 智之,熱海 悠子,杉原 英志,佐谷 秀行,菅野 雅元,田代 文夫,益谷 美都子,吉岡 研一
  • Organizer: 第３５回日本分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 抗癌剤存在下での優先的な癌細胞死と正常細胞の生存に資するArf/p53依存的H2AX制御機構 (2012)
  • Author(s): 熱海 悠子,稲瀬 安希,杉原 英志,佐谷 秀行,菅野 雅元,益谷 美都子,吉岡 研一
  • Organizer: 第３５回日本分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] UVに応答した核内のIKKbetaを介したNF-kappaB活性化と細胞死 (2012)
  • Author(s): 土谷 佳弘,金本 麻裕,浅野 知一郎,菅野 雅元,鎌田 英明
  • Organizer: 第３５回日本分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] The function of Bmi-1 in the thymic epithelial cells (2011)
  • Author(s): ・ Yun Guo, Masaki Miyzaki, Manami Itoi, Takashi Amagai, Rumi Satoh, Hiroshi Kawamoto, Masamoto Kanno
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張メッセ
• [Presentation] The impact of metabolism of methylation: importance of methyl donor for epigenetic reggulation of lymphocyte (2011)
  • Author(s): masamoto KANNO
  • Organizer: kyoto T cell conference
  • Place of Presentation: 京都平安ホテル