Quality control of iPS cells by studying differentiation resistance.
Project/Area Number |
23618011
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Regenerative medicine
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Research Institution | Keio University |
Principal Investigator |
NAGAMATSU Go 慶應義塾大学, 医学部, 助教 (70453545)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 幹細胞 / 多能性 / リプログラミング / 分化 / 安全性 |
Research Abstract |
A tagging system was developed to sort induced pluripotent stem (iPS) cells according to the expression levels of each of the four reprogramming factors. Using this system, the effective ratio (Oct3/4-high, Sox2-low, Klf4-high, c-Myc-high) was. To investigate the molecular basis, microarray analysis was performed. Pathway analysis revealed that the G protein-coupled receptor (GPCR) pathway was up-regulated significantly under the high efficiency condition and treatment with the chemokine, C-C motif ligand 2, a member of the GPCR family, enhanced somatic cell reprogramming. Furthermore, the genetic modifier, Whsc1l1 (variant 1), also improved the efficiency of somatic cell reprogramming.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Induction of pluripotent stem cells from primordial germ cells by single reprogramming factors2013
Author(s)
Nagamatsu G, Kosaka T, Saito S, Honda H, Takubo K, Kinoshita T, Akiyama H, Sudo T, Horimoto K, Oya M, Suda T
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Journal Title
Stem Cells
Volume: 31(3)
Pages: 479-87
Related Report
Peer Reviewed
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[Journal Article] Posttranscriptional Regulation of Histone Lysine Methyltransferase GLP in Embryonic Male Mouse Germ Cells.2013
Author(s)
Deguchi K, Nagamatsu G, Miyachi H, Kato Y, Morita S, Kimura H, Kitano S, Hatada I, Saga Y, Tachibana M, Shinkai Y.
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Journal Title
Biol Reprod
Volume: 88
Issue: 2
Pages: 36-36
DOI
Related Report
Peer Reviewed
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