Optogenetic approach of gene regulation
Project/Area Number |
23650172
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neuroscience in general
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Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
HAYASHI Yasunori 独立行政法人理化学研究所, 記憶メカニズム研究チーム, チームリーダー (90466037)
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Project Period (FY) |
2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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Keywords | 遺伝子発現 / Cre / LOVドメイン / 光遺伝学 / Creレコンビナーゼ |
Research Abstract |
In order to generate photoactivatable Cre, we made a series of fusion proteins between LOV domain and Cre and introduce into HEK cells along with lox-stop-lox-GFP, which allows detection of Cre activity by GFP. However, irrespective illumination, we saw GFP signal, indicating that LOV domain did not effectively suppress the activity of Cre. However, in some of constructs, GFP was dimmer than others. We will further pursue these constructs to see if we can improve them by adding additional mutations.
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Report
(2 results)
Research Products
(22 results)
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[Journal Article] A rapid and effective FISH protocol with hybridization-sensitive fluorescent linear oligo probes2012
Author(s)
Wang, D. O., Matsuno, H., Ikeda, S., Nakamura, A., Yanagisawa, H., Hayashi, Y., Okamoto, A
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Journal Title
RNA
Volume: 18
Pages: 166-175
Related Report
Peer Reviewed
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[Journal Article] Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo2011
Author(s)
Mower, A. F., Kwok, S. M., Yu, H., Majewska, A. K., Okamoto, K.-I., Hayashi, Y., Sur, M.
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Journal Title
Proc. Natl. Acad. Sci
Volume: 108
Pages: 21241-21246
Related Report
Peer Reviewed
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[Journal Article] Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo2011
Author(s)
Mower, A.F., Kwok, S.M., Yu, H., Majewska, A.K., Okamoto, K.-I., Hayashi, Y.*, Sur, M.* (*: co-corresponding authors)
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Journal Title
Proc.Natl.Acad.Sci.
Volume: 108
Issue: 52
Pages: 21241-21246
DOI
Related Report
Peer Reviewed
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[Presentation] CaMKII gates rapid structural plasticity in hippocampal dendritic spines2011
Author(s)
Kim, K., Hayashi, M., Narayanan, R., Suzuki, A., Matsuura, K., Okamoto, K., Hayashi, Y.
Organizer
SfN2011
Place of Presentation
Washington DC
Year and Date
2011-11-16
Related Report
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[Presentation] The role of CaMKII beta in modulating dendritic spine structural plasticity2011
Author(s)
Lakhanpal, G., Kim, K., Kato-Hayashi, M., Hayashi, Y., Okamoto, K.
Organizer
SfN 2011
Place of Presentation
Washington DC, USA
Year and Date
2011-11-16
Related Report
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[Presentation] CaMKII gates rapid structural plasticity in hippocampal dendritic spines2011
Author(s)
Kim, K., Hayashi, M., Narayanan, R., Suzuki, A., Matsuura, K., Okamoto, K., Hayashi, Y.
Organizer
SfN 2011
Place of Presentation
Washington DC, USA
Year and Date
2011-11-16
Related Report
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[Presentation] In vivo two-photon imaging of neuronal circuit activity using transgenic mice that express fluorescent calcium sensor proteins in the brain2011
Author(s)
Sato, M., Kawano, M., Islam, T., Yamaguchi, Y., Ohkura, M., Nakai, J., Hayashi, Y.
Organizer
第34回日本神経科学大会
Place of Presentation
横浜
Year and Date
2011-09-16
Related Report
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[Presentation] The regulation of dendritic spine structural plasticity by CaMKII beta2011
Author(s)
Lakhanpal, G. K., Kim, K., Kato-Hayashi, M., Hayashi, Y.,& Okamoto, K
Organizer
8th IBRO World Congress of Neuroscience
Place of Presentation
Florence, Italy
Year and Date
2011-07-15
Related Report
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[Presentation] The regulation of dendritic spine structural plasticity by CaMKII beta2011
Author(s)
Lakhanpal, G.K., Kim, K., Kato-Hayashi, M., Hayashi, Y., Okamoto, K
Organizer
8^<th> IBRO World Congress of Neuroscience
Place of Presentation
Florence, Italy
Year and Date
2011-07-15
Related Report
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