Functional connectomics analysis of synaptic transmission using optogenetics
| Project/Area Number |
23650208
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
KATO Fusao 東京慈恵会医科大学, 医学部, 教授 (20169519)
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| Co-Investigator(Renkei-kenkyūsha) |
WATABE M.ayako 東京慈恵会医科大学, 医学部, 准教授 (00334277)
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| Research Collaborator |
TAKAHASHI Yukari 東京慈恵会医科大学, 医学部, 助教 (20613764)
SUGIMURA Yae 東京慈恵会医科大学, 大学院・日本学術振興会, 特別研究員
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 光脳科学 / 神経回路 / 光遺伝学 / 脳 / 扁桃体 / 腕傍核 / シナプス / チャネルロドプシン / シナプス伝達 / 脳スライス / 神経投射 / アデノ随伴ウィルス / feed-forward inhibition / 機能コネクトミクス / 神経科学 / コネクトミクス / 機能的コネクトーム / 逆行性標識 |
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| Research Abstract |
The aim was to develop a method applicable to slice-based analysis of synaptic transmission using optogenetics that can determine the origin of fibers being stimulated. Five to eight weeks after transfecting adeno-associated virus vectors for ChR2 expression into the lateral parabrachial nucleus (LPB) in the dorsal pons of rats, blue light pulses robustly triggered CNQX-sensitive EPSCs in the neurons in the capsular part of the central amygdala recorded in acute slices of distinct angles (coronal and horizontal). These light-evoked EPSCs (leEPSCs) were blocked by TTX but re-appeared by addition of 4-AP, suggesting monosynaptic innervation, and was followed by a large and long-lasting IPSC, indicative of the presence of polysynaptic feed-forward inhibition. This "functional connectomics" technique has advantages in identifying the synaptic properties of long-distance connections, which has been otherwise almost impossible in the conventional electrophysiology setups.
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Report
(4 results)
Research Products
(14 results)
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[Presentation] Bilateral potentiation of parabrachial, but not basolateral amygdala inputs, to central capsular amygdala neurons in neuropathic diabetic mice,2012
Author(s)
Ochiai Y, Takahashi Y, Asato M, Watabe AM Ohsawa M, Kamei J, Kato F
Organizer
Society for Neuroscience Annual Meeting 2012
Place of Presentation
New Orleans, LA, USA
Related Report
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