Functional analysis of DNA methylation in post-mitotic neurons

• Project/Area Number: 23650215
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Fusional basic brain science
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: NAMIHIRA Masakazu 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (60379534)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ニューロン / 発達 / DNA メチル化 / エピジェネティクス / DNA ヒドロキシメチル化 / DNAメチル化 / DNAヒドロキシメチル化

Research Abstract

Although neurons are postmitotic cells, which completely have exited cell cycle, the expression of DNA methyltransferase 1 (DNMT1) is observed in the cells. However, the function of DNMT1 in postmitotic neurons is unclear. To address this issue, we deleted Dnmt1 gene in neurons and examined the resultant phenotype in vitro.We found that DNMT1 plays an important role in cortical development through the regulation of neurite outgrowth mediated by neurotrophic factor signaling

Research Products

• [Journal Article] Chromatin accessibility at a STAT3 target site is altered prior to astrocyte differentiation (2013)
  • Author(s): Urayama S, Semi K, Sanosaka T, Hori Y,Namihira M, Kohyama J, Takizawa T,Nakashima K.
  • Journal Title: Cell StructFunct Volume: 38(1) Pages: 55-66
  • NAID: 130004053894
  • URL: https://www.jstage.jst.go.jp/article/csf/38/1/38_12034/_article
  • Peer Reviewed
• [Journal Article] Chromatin accessibility at a STAT3 target site is altered prior to astrocyte differentiation. (2013)
  • Author(s): Urayama S, Semi K, Sanosaka T, Hori Y, Namihira M, Kohyama J, Takizawa T, Nakashima K.
  • Journal Title: Cell Structure and Function Volume: 38 Pages: 55-66
  • NAID: 130004053894
  • Peer Reviewed
• [Journal Article] Dnmt3a regulates both proliferation and differentiation of mouse neural stem cells. (2012)
  • Author(s): Wu Z, Huang K, Yu J, Le T, Namihira M, Liu Y, Zhang J, Xue Z, Cheng L, Fan G.
  • Journal Title: Journal of Neuroscience Research Volume: 90 Issue: 10 Pages: 1883-1891
  • DOI: 10.1002/jnr.23077
  • Peer Reviewed
• [Journal Article] Treatment of a mouse model of spinal cord injury by transplantation of human iPS cell-derived long-term self-renewing neuroepithelial-like stem cells (2012)
  • Author(s): Fujimoto Y., Abematsu M., FalkA., Tsujimura K., Sanosaka T., Juliandi B.. Semi K., Namihira M., Komiya S., SmithA., Nakashima K,
  • Journal Title: Stem Cells Volume: 30 Issue: 6 Pages: 1163-1173
  • DOI: 10.1002/stem.1083
  • Peer Reviewed
• [Presentation] DNA メチル化による神経発生制御機構 (2013)
  • Author(s): 波平昌一
  • Organizer: 奈良先端未来開拓コロキウム「シグナルの破綻による疾患の生物学」
  • Place of Presentation: 奈良県生駒市
  • Year and Date: 2013-01-28
• [Presentation] Treatment of a mouse model of spinal cord injury by transplantation of human iPS cell-derived long term self-renewing neuroepithelial-like stem cells (2012)
  • Author(s): Fujimoto, Y., Abematsu, M., Falk, A., Tsujimura,K., Sanosaka, T., Juliandi, B., Semi, K., Namihira,M., Komiya, S., Smith, A., Nakashima, K.
  • Organizer: NEUROSCIENCE 2012
  • Place of Presentation: New Orleans
  • Year and Date: 2012-10-15
• [Presentation] TLR9-signaling regulates neurogenesis in the adult mouse hippocampus (2012)
  • Author(s): Matsuda, T., Murao, N., Juliandi, B.,Namihira, M., Kawai, T., Akira, S.,Nakashima, K.
  • Organizer: NEUROSCIENCE 2012
  • Place of Presentation: New Orleans
  • Year and Date: 2012-10-13
• [Presentation] マウス脳におけるNP95 発現細胞の解析. (2012)
  • Author(s): 村尾直哉、松田泰斗、古関明彦、波平昌一、中島欽一
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2012-09-18
• [Presentation] グリア細胞の分化と発達におけるDNA メチル化の役割 (2012)
  • Author(s): 石川寛、波平昌一、竹林浩秀、中島欽一
  • Organizer: 包括脳ネットワーク夏のワークショップ
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2012-07-25
• [Presentation] ニューロンの発達及び興奮毒性神経細胞死制御におけるDNMT1 の機能解析 (2012)
  • Author(s): 赤土正一、田中友規、波平昌一、野口浩史、五十嵐勝秀、辻村啓太、中島欽一
  • Organizer: 包括脳ネットワーク夏のワークショップ
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2012-07-25
• [Presentation] REGULATION OF ADULT MOUSE NEURAL STEM CELLS BY TLR9 MEDIATED SIGNALING (2012)
  • Author(s): Matsuda T., Juliandi B., Murao N., Namihira M., Kawai T., Akira S., Nakashima K
  • Organizer: ISSCR 2012
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2012-06-13
• [Presentation] MENINGEAL CELLS INDUCE ASTROCYTE DIFFERENTIATION OF NEURAL STEM CELLS (2012)
  • Author(s): Sanosaka T., Namihira M., Nakashima K
  • Organizer: ISSCR 2012
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2012-06-13
• [Presentation] Meningeal cells regulate astrocytic differentiation in the embryonic mouse brain (2012)
  • Author(s): 佐野坂司、波平昌一、中島欽一
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2012-05-28
• [Presentation] DNA-methyltransferase 1 functions as a suppressor of neuron ldifferentiation in late-gestationalneural stem cells. (2012)
  • Author(s): 野口浩史、波平昌一、田中友規、佐野坂司、中島欽一
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2012-05-28
• [Presentation] マウス脳におけるNP95 発現細胞の解析 (2012)
  • Author(s): 村尾直哉、松田泰斗、古関明彦、波平昌一、中島欽一
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京都千代田区
  • Year and Date: 2012-05-14
• [Presentation] DNMT1 regulates neuronal differentiation of neural precursor cell in late-gestational forebrain. (2012)
  • Author(s): Noguchi, H., Namihira, M., Tanaka, T., Sanosaka, T., Nakashima, K.
  • Organizer: 40th KEYSTONE SYMPOSIA
  • Place of Presentation: Keystone, Colorado, USA
• [Presentation] Treatment of a mouse model of spinal cord injury by transplantation of human iPS cell-derived long-term self-renewing neuroepithelial-like stem cells
  • Author(s): Fujimoto, Y., Abematsu, M., Falk, A., Tsujimura, K., Sanosaka, T., Juliandi, B., Semi, K., Namihira, M., Komiya, S., Smith, A., Nakashima, K.
  • Organizer: NEUROSCIENCE 2012
  • Place of Presentation: New Orleans
• [Presentation] TLR9-signaling regulates neurogenesis in the adult mouse hippocampus.
  • Author(s): Matsuda, T., Murao, N., Juliandi, B., Namihira, M., Kawai, T., Akira, S., Nakashima, K.
  • Organizer: NEUROSCIENCE 2012
  • Place of Presentation: New Orleans
• [Presentation] マウス脳におけるNP95発現細胞の解析
  • Author(s): 村尾直哉、松田泰斗、古関明彦、波平昌一、中島 欽一
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京都千代田区
• [Presentation] DNA-methyltransferase 1 functions as a suppressor of neuronal differentiation in late-gestational neural stem cells
  • Author(s): 野口浩史、波平昌一、田中友規、佐野坂司、中島欽一
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 兵庫県神戸市
• [Presentation] MENINGEAL CELLS INDUCE ASTROCYTE DIFFERENTIATION OF NEURAL STEM CELLS
  • Author(s): Sanosaka T., Namihira M., Nakashima K.
  • Organizer: ISSCR 2012
  • Place of Presentation: 神奈川県横浜市
• [Presentation] グリア細胞の分化と発達におけるDNAメチル化の役割
  • Author(s): 石川寛、波平昌一、竹林浩秀、中島欽一
  • Organizer: 包括脳ネットワーク夏のワークショップ
  • Place of Presentation: 宮城県仙台市
• [Presentation] ニューロンの発達及び興奮毒性神経細胞死制御におけるDNMT1の機能解析
  • Author(s): 赤土正一、田中友規、波平昌一、野口浩史、五十嵐勝秀、辻村啓太、中島欽一
  • Organizer: 包括脳ネットワーク夏のワークショップ
  • Place of Presentation: 宮城県仙台市
• [Presentation] マウス脳におけるNP95発現細胞の解析
  • Author(s): 村尾直哉、松田泰斗、古関明彦、波平昌一、中島 欽一
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 愛知県名古屋市
• [Presentation] DNAメチル化による神経発生制御機構
  • Author(s): 波平昌一
  • Organizer: 奈良先端未来開拓コロキウム「シグナルの破綻による疾患の生物学」
  • Place of Presentation: 奈良県生駒市
  • Invited
• [Presentation] 転写因子NFIAによって誘導されるOlig1遺伝子プロモーターの脱メチル化
  • Author(s): 蝉克憲、佐野坂司、波平昌一、畑田出穂、中島欽一
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: KKRホテル熊本
• [Presentation] DNAメチル化による神経発生制御機構
  • Author(s): 波平昌一、Fan Guoping、中島欽一
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: KKRホテル熊本
• [Presentation] 転写因子が誘導するOlig1遺伝子プロメーターの脱メチル化
  • Author(s): 蝉克憲、佐野坂司、波平昌一、畑田出穂、中島欽一
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Roles of DNA Methylation and Methyltransferases in Neural Development
  • Author(s): Namihira M
  • Organizer: 第34回日本神経科学大会(招待講演)
  • Place of Presentation: パシフィコ横浜
• [Presentation] Functional analysis of DNA-methyltranseferase 1 in post-mitotic neurons
  • Author(s): 田中友規、波平昌一、野口浩史、五十嵐勝秀、辻村啓太、中島欽一
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] DNMT1 inhibits the neuronal differentiation of late-gestational neural precursor cells
  • Author(s): 野口浩史、波平昌一、田中友規、佐野坂司、中島欽一
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] DNMT1 regulates neuronal differentiation of neural precursor cell in late-gestational forebrain
  • Author(s): 波平昌一、野口浩史、田中友規、佐野坂司、中島欽一
  • Organizer: 1st International Symposium/59th NIBB Conference NEOCORTICAL ORGANIZATION
  • Place of Presentation: 岡崎コンファレンスセンター
• [Presentation] NFIA-induced DNA demethylation in Olig1 promoter regulates the expression of the gene in late-gestational neural precursor cells
  • Author(s): 蝉克憲、佐野坂司、波平昌一、畑田出穂、中島欽一
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Book] Neurogenesis in the adult brain I (eds. Seki, T., Sawamoto, K., Parent, J.M. & Alvarez-Buylla, A.) (2011)
  • Author(s): Namihira M, Nakashima K.
  • Total Pages: 21
  • Publisher: 東京スプリンガー