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Identification of novel homing molecules of hematopoietic stem cells and development of efficient bone marrow transfer methods by modifying carbohydrates

Research Project

Project/Area Number 23650232
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionKanazawa University

Principal Investigator

ASANO Masahide  金沢大学, 学際科学実験センター, 教授 (50251450)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Noriyoshi  金沢大学, 金沢大学, 准教授 (50242524)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords糖鎖 / 骨髄幹細胞 / 骨髄移植 / ホーミング
Research Abstract

We have found that beta4GalT-I-deficient bone marrow (BM) cells cannot engraft in the BM of X-ray radiated wild-type mice. Not only beta4GalT-I-deficient BM cells cannot establish hematopoietic system of recipient mice, but also their engraftment into the BM shortly after BM transfer was markedly reduced, indicating that homing of hematopoietic stem cells (HSC) into the BM was impaired. We next investigated mice having a point mutation in the GNE gene, that encodes a key enzyme for sialic acids biosynthesis. Homing efficiency of BM cells from GNE point-mutant mice was reduced to half that of wild-type mice, although the reduction was not prominent as in beta4GalT-I-deficient mice. These results suggest that sialic acids at the terminus of carbohydrate chains also play a role in HSC homing.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (17 results)

All 2014 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (10 results) Remarks (3 results)

  • [Journal Article] Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach2012

    • Author(s)
      Sugahara, D., Kaji, H., Sugihara, K., Asano, M., and Narimatsu, H.
    • Journal Title

      Scientific Reports

      Volume: 2 Issue: 1 Pages: 680-680

    • DOI

      10.1038/srep00680

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Altered oligosaccharide structures contribute to ameliorate murine colitis defective in beta 1,4 galactosyltransferase I.2012

    • Author(s)
      Shinzaki S, Miyoshi E, et al.
    • Journal Title

      Gastroenterology

      Volume: 142(5) Issue: 5 Pages: 1172-82

    • DOI

      10.1053/j.gastro.2012.02.008

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Glycoprotein hyposialylation gives rise to a nephrotic-like syndrome that is prevented by sialic acid administration in GNE V572L point-mutant mice2012

    • Author(s)
      Ito, M., Sugihara, K., Asaka, T., Toyama, T., Yo sh ih ar a, T., Furuichi, K., Wada, T. and Asano, M.
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 1 Pages: 1-13

    • DOI

      10.1371/journal.pone.0029873

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 4-galactosyltransferase-II and GlcAT-PFacilitates Biosynthesis of N-linked HNK-1Carbohydrate2011

    • Author(s)
      N. Nakagawa, T. Yoshihara, M. Asano, and S. Oka
    • Journal Title

      J. Biol. Chem

      Volume: 286(36) Issue: 36 Pages: 31337-46

    • DOI

      10.1074/jbc.m111.233353

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 造血幹細胞の骨髄ホーミングにおける糖鎖の役割2014

    • Author(s)
      橋本憲佳, 田畑佳祐, 高垣聡一郎, 西江敏和, 浅野雅秀
    • Organizer
      第61回日本実験動物学会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-05-15
    • Related Report
      2013 Final Research Report
  • [Presentation] 造血幹細胞の骨髄ホーミングにおける糖鎖の役割2014

    • Author(s)
      橋本憲佳,田畑佳祐,高垣聡一郎,西江敏和,浅野雅秀
    • Organizer
      第61回日本実験動物学会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] β-1, 4-ガラクトース転移酵素-Iが作る糖鎖は造血幹細胞の骨髄ニッチへのホーミングに必須である2012

    • Author(s)
      田畑佳祐, 高垣聡一郎, 西江敏和, 浅野雅秀
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2012-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] シアル酸糖鎖欠損による腎疾患モデルマウスの病態解析とシアル酸投与による治療効果2012

    • Author(s)
      伊藤光俊, 浅賀知也, 杉原一司, 吉原亨, 和田隆志, 浅野雅秀
    • Organizer
      第2回分子腎臓フォーラム
    • Place of Presentation
      京都リサーチパーク(京都府)
    • Year and Date
      2012-01-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Hyposialylation of glycoproteins in the UDP-N-acetylglucosamine-2-epimerase/N -acetylmannosamine kinase (GNE) point mutant mice gives rise to a renal impairment that is prevented by sialic acid administration2011

    • Author(s)
      Ito, M., Asaka, T ., Sugihara, K., Y oshihara, T ., Wada, T . and Asano, M
    • Organizer
      Kidney Week 2011
    • Place of Presentation
      Pennsylvania Convention Center , Philadelphia, USA
    • Year and Date
      2011-11-11
    • Related Report
      2013 Final Research Report
  • [Presentation] GNE V572L 点変異マウスにおける腎臓の病態解析とシアル酸糖鎖の関与2011

    • Author(s)
      伊藤光俊, 浅賀知也, 杉原一司, 吉原亨, 和田隆志, 浅野雅秀
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2011-09-24
    • Related Report
      2013 Final Research Report
  • [Presentation] GNE V572L 点変異マウスにおける腎臓の病態解析とシアル酸糖鎖の関与2011

    • Author(s)
      伊藤光俊ら
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Related Report
      2011 Research-status Report
  • [Presentation] Hyposialylation of glycoproteins in the UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE) point mutant mice gives rise to a renal impairment that is prevented by sialic acid administration2011

    • Author(s)
      Ito, M., et al.
    • Organizer
      Kidney Week 2011
    • Place of Presentation
      Pennsylvania Convention Center (USA)
    • Related Report
      2011 Research-status Report
  • [Presentation] 行動テストバッテリーによる神経特異的β4GalT-5欠損マウスの高次脳機能の解析2011

    • Author(s)
      鈴木紘史ら
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] β-1,4-ガラクトース転移酵素-Iが作る糖鎖は造血幹細胞の骨髄ニッチへのホーミングに必須である

    • Author(s)
      田畑佳祐,高垣聡一郎,西江敏和,浅野雅秀
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://asrc.w3.kanazawa-u.ac.jp/

    • Related Report
      2013 Final Research Report
  • [Remarks] 学際科学実験センター遺伝子改変動物分野

    • URL

      http://asrc.w3.kanazawa-u.ac.jp/

    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://asrc.w3.kanazawa-u.ac.jp/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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