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Establishment of protocol for building up monoclonal antibodies targeting cancer stem cells

Research Project

Project/Area Number 23650608
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Tumor immunology
Research InstitutionOkayama University

Principal Investigator

SAKAGUCHI Masakiyo  岡山大学, 大学院・医歯薬学総合研究科, 准教授 (70379840)

Co-Investigator(Kenkyū-buntansha) KATAOAKA Ken  岡山理科大学, 理学部, 准教授 (10293317)
MURATA Hitoshi  岡山大学, 大学院・医歯薬学総合研究科, 助教 (90579096)
HUH Namho  岡山大学, 大学院・医歯薬学総合研究科, 教授 (70142023)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsがん幹細胞 / 抗体 / 膜タンパク質 / 腫瘍免疫
Research Abstract

In this study, we succeeded to establish a cancer stem cell (CSC) clone by the innovative plasmid construct. Now, we are in the process of immunization of functional membrane proteins derived from the established CSC clone to generate a panel of monoclonal antibodies against CSCs.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (43 results)

All 2013 2012 2011 Other

All Journal Article (20 results) (of which Peer Reviewed: 20 results) Presentation (18 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (5 results)

  • [Journal Article] Coxsackie and adenovirus receptor is a critical regulator for the survival and growth of oral squamous carcinoma cells2013

    • Author(s)
      Saito K and Sakaguchi M
    • Journal Title

      Oncogene

      Volume: in press Issue: 10 Pages: 1274-1286

    • DOI

      10.1038/onc.2013.66

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] DOCK7 is a critical regulator of the RAGE-Cdc42 signaling axis that induces formation of dendritic pseudopodia in human cancer cells2013

    • Author(s)
      Yamamoto K, Murata H, Putranto EW, Kataoka K, Motoyama A, Hibino T, Inoue Y, Sakaguchi M, Huh NH
    • Journal Title

      Oncol Rep

      Volume: 29 Pages: 1073-1079

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] S100A9 is a novel ligand of EMMPRIN that promotes melanoma metastasis2013

    • Author(s)
      Hibino T, Sakaguchi M, Miyamoto S, Yamamoto M, Motoyama A, Hosoi J, Shimokata T, Ito T, Tsuboi R, Huh NH
    • Journal Title

      Cancer Res

      Volume: 73 Pages: 172-183

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preclinical biodistribution and safety study of reduced expression in immortalized cells/Dickkopf-3-encoding adenoviral vector for prostate cancer gene therapy2012

    • Author(s)
      Sugimoto M, Watanabe M, Kaku H, Li SA, Noguchi H, Ueki H, Sakaguchi M, Huh NH, Nasu Y, Kumon H
    • Journal Title

      Int J Oncol

      Volume: 28 Pages: 1645-1652

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] REIC/Dkk-3-encoding adenoviral vector as a potentially effective therapeutic agent for bladder cancer2012

    • Author(s)
      Hirata T, Watanabe M, Kaku H, Kobayashi Y, Yamada H, Sakaguchi M, Takei K, Huh NH, Nasu Y, Kumon H
    • Journal Title

      Int J Oncol

      Volume: 41 Pages: 559-564

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] S100A7 promotes the migration and invasion of osteosarcoma cells via the receptor for advanced glycation end products2012

    • Author(s)
      Kataoka K, Ono T, Murata H, Morishita M, Yamamoto K, Sakaguchi M, Huh NH
    • Journal Title

      Oncol Lett

      Volume: 3 Pages: 1149-1153

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Preclinical Safety and Efficacy of in Situ REIC/Dkk-3 Gene Therapy for Prostate Cancer2012

    • Author(s)
      Kawauchi K, Watanabe M, Kaku H, Huang P, Sasaki K, Sakaguchi M, Ochiai K, Huh NH, Nasu Y, Kumon H
    • Journal Title

      Acta Med Okayama

      Volume: 66 Pages: 7-16

    • NAID

      120003850603

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression pattern of REIC/Dkk-3 in mouse squamous epithelia2012

    • Author(s)
      Kataoka K, Du G, Maehara N, Murata H, Sakaguchi M, Huh N
    • Journal Title

      Clin Exp Dermatol

      Volume: 37 Pages: 428-431

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Partial sensitization of human bladder cancer cells to a gene-therapeutic adenovirus carrying REIC/Dkk-3 by downregulation of BRPK/PINK12012

    • Author(s)
      Jin Y, Murata H, Sakaguchi M, Kataoka K, Watanabe M, Nasu Y, Kumon H, Huh NH
    • Journal Title

      Oncol Rep

      Volume: 27 Pages: 695-699

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression pattern of REIC/Dkk-3 in mouse squamous epithelia.2012

    • Author(s)
      Kataoka K, Du G, Maehara N, Murata H, Sakaguchi M, Huh N
    • Journal Title

      Clin Exp Dermatol

      Volume: in press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-12011

    • Author(s)
      Ochiai K, Watanabe M, Ueki H, Huang P, Fujii Y, Nasu Y, Noguchi H, Hirata T, Sakaguchi M, Huh NH, Kashiwakura Y, Kaku H, Kumon H
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 412 Pages: 391-395

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] TIRAP, an Adaptor Protein for TLR2/4, Transduces a signal from RAGE Phosphorylated upon Ligand Binding2011

    • Author(s)
      Sakaguchi M, Murata M, Yamamoto K, Ono T, Sakaguchi Y, Motoyama A, Hibino T, Kataoka K, Huh NH
    • Journal Title

      PLoS ONE

      Volume: 6

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Epigenetic silencing of microRNA-34b/c plays an important role in the pathogenesis of malignant pleural mesothelioma2011

    • Author(s)
      Kubo T, Toyooka S, Tsukuda K, Sakaguchi M, Fukazawa T, Soh J, Asano H, Ueno T, Muraoka T, Yamamoto H, Nasu Y, Kishimoto T, Pass HI, Matsui H, Huh NH, Miyoshi S
    • Journal Title

      Clin Cancer Res

      Volume: 17 Pages: 4965-4974

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Markedly elevated serum levels of calcium-binding S100A8/A9 proteins in psoriatic arthritis are due to activated monocytes/macrophages2011

    • Author(s)
      Aochi S, Tsuji K, Sakaguchi M, Huh NH, Tsuda T, Yamanishi K, Komine M, Iwatsuki K
    • Journal Title

      J Am Acad Dermatol

      Volume: 64 Pages: 879-887

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Intraperitoneal administration of an adenovirus vector carrying REIC/Dkk-3 suppresses peritoneal dissemination of scirrhous gastric carcinoma2011

    • Author(s)
      Than SS, Kataoka K, Sakaguchi M, Murata H, Abarzua F, Taketa C, Du G, Yashiro M, Yanagihara K, Nasu Y, Kumon H, Huh NH
    • Journal Title

      Oncol Rep

      Volume: 25 Pages: 989-995

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A new cytosolic pathway from a Parkinson's disease-associated kinase, BRPK/PINK1: activation of AKT via MTORC22011

    • Author(s)
      Murata H, Sakaguchi M, Jin Y, Sakaguchi Y, Futami JI, Yamada H, Kataoka K, Huh NH
    • Journal Title

      J Biol Chem

      Volume: 286 Pages: 7182-7189

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression of REIC/Dkk-3 in normal and hyperproliferative epidermis2011

    • Author(s)
      Du G, Kataoka K, Sakaguchi M, Abarzua F, Than SS, Sonegawa H, Makino T, Shimizu T, Huh NH
    • Journal Title

      Exp Dermatol

      Volume: 20 Pages: 273-277

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Novel Tumor Suppressor, REIC/Dkk-3 Gene Identified by Our In Vitro Transformation Model of Normal Human Fibroblasts Works as a Potent Therapeutic Anti-tumor Agent2011

    • Author(s)
      Sakaguchi M, Huh NH, Namba M
    • Journal Title

      Adv Exp Med Biol

      Volume: 720 Pages: 209-215

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] S100A11, a dual growth regulator of epidermal keratinocytes2011

    • Author(s)
      Sakaguchi M, Huh NH
    • Journal Title

      Amino Acids

      Volume: 41 Pages: 797-807

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Multiple functions of PINK1 at different intracellular locations: Beyond neuro-degenerative diseases2011

    • Author(s)
      Murata H, Sakaguchi M, Kataoka K, Huh NH
    • Journal Title

      Cell Cycle

      Volume: 10 Pages: 1518-1519

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Identification of novel receptors for pro-inflammatory S100A8/A9 proteins and their roles inmelanoma metastasis2012

    • Author(s)
      阪口政清
    • Organizer
      IEIIS2012. Homeostatic Inflammation Symposium
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] S100A8 および S100A9 タンパク質の新規受容体の探索とそのがん進展における役割2012

    • Author(s)
      阪口政清
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] A Novel Tumor Suppressor, REIC/Dkk-3 Gene Identified by our in Vitro Transformation Model of Normal Human Fibroblasts Works as a Potent Therapeutic Anti-tumor Agent2012

    • Author(s)
      阪口政清
    • Organizer
      2012 World Congress on In Vitro Biology
    • Place of Presentation
      アメリカ、Bellevue(招待講演)
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞内導入型RAGE阻害ペプチドの開発2012

    • Author(s)
      村田等
    • Organizer
      日本生物工学会西日本支部創立30周年記念シンポジウム・講演会
    • Place of Presentation
      岡山市
    • Related Report
      2012 Annual Research Report
  • [Presentation] A Novel Tumor Suppressor, REIC/Dkk-3 Gene Identified by our in Vitro Transformation Model of Normal Human Fibroblasts Works as a Potent Therapeutic Anti-tumor Agent2012

    • Author(s)
      阪口政清
    • Organizer
      2012 World Congress on In Vitro Biology
    • Place of Presentation
      アメリカ、Bellevue
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] S100A8およびS100A9タンパク質の新規受容体の探索とそのがん進展における役割2012

    • Author(s)
      阪口政清
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] DOCK7 is a critical regulator of the RAGE-Cdc42 signaling axis that induces formation of dendritic pseudopodia in human cancer cells2012

    • Author(s)
      山本健一
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] TRAF6 Ubiquitinates and Stabilizes PINK1 on Damaged Mitochondria Through Interaction with SARM12012

    • Author(s)
      村田 等
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] 多機能受容体 RAGE の下流信号伝達機構の解明2011

    • Author(s)
      阪口政清
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Final Research Report
  • [Presentation] REIC/Dkk-3 の扁平上皮における発現とその制御因子の探索

    • Author(s)
      片岡 健, 阪口政清, 杜 剛, 前原奈都美, 村田 等, 許 南浩
    • Organizer
      日本組織培養学会第84回大会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] "PINK1 knockdown increases the apoptosis induced by adenovirus-mediated REIC/Dkk-3 overexpression on bladder cancer cells "

    • Author(s)
      "YU JIN, HITOSHI MURATA, KEN KATAOKA, MASAKIYO SAKAGUCHI, NAM-HO HUH"
    • Organizer
      日本組織培養学会第84回大会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] S100A7による骨肉腫細胞の遊走・浸潤の多機能受容体RAGEを介した制御機構

    • Author(s)
      片岡健、阪口政清、小野智之、森下美加、山本健一、村田等、許南浩
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Research-status Report
  • [Presentation] "PINK1発現抑制によるミトコンドリア機能不全はREIC/Dkk-3のアポトーソシス誘導効果を増強する

    • Author(s)
      村田等、金玉、阪口政清、片岡健、許南浩
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Research-status Report
  • [Presentation] 多機能受容体RAGEの下流信号伝達機構の解明

    • Author(s)
      阪口政清、村田等、山本健一、阪口義彦本山晃、日比野利彦、片岡健 許南浩
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Research-status Report
  • [Presentation] 正常皮膚におけるREIC/Dkk-3の発現制御因子の検索

    • Author(s)
      前原奈都美、片岡健、杜剛、村田等、山本健一、阪口政清、許南浩
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] Mitochondrial dysfunction by PINK1 knockdown augments apotosis induced by a gene-therspeutic adenovirus carrying REIC/Dkk-3

    • Author(s)
      Hitoshi Murata, Jin Yu, Ken Kataoka, Masakiyo Sakaguchi, Nam-ho Huh
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] RAGE高発現がもたらす細胞形態変化と細胞運動亢進の分子機構の解析

    • Author(s)
      山本健一、阪口政清、許南浩
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] S100タンパク質受容体の統合解析

    • Author(s)
      森下美加、村田等、山本健一、片岡健、阪口政清、許南浩
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Patent(Industrial Property Rights)] NPTNβとS100A8の結合の阻害を指標とする細胞増殖抑制剤のスクリーニング方法2012

    • Inventor(s)
      阪口政清、日比野利彦、山本真実、許南浩
    • Industrial Property Rights Holder
      阪口政清、日比野利彦、山本真実、許南浩
    • Industrial Property Number
      2012-204279
    • Filing Date
      2012-09-18
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] PINK1のユビキチンアッセイ及びスクリーニングへの利用2012

    • Inventor(s)
      村田 等、阪口政清、許南浩
    • Industrial Property Rights Holder
      村田 等、阪口政清、許南浩
    • Industrial Property Number
      2012-165160
    • Filing Date
      2012-07-25
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] アデノウイルスベクターをがん細胞に対して選択的に導入可能なポリペプチドおよび当該ポリペプチドを備えるアデノウイルスベクター2012

    • Inventor(s)
      阪口政清、近藤英作、許南浩、手塚克成
    • Industrial Property Rights Holder
      阪口政清、近藤英作、許南浩、手塚克成
    • Industrial Property Number
      2012-085969
    • Filing Date
      2012-04-04
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Patent(Industrial Property Rights)] PINK1のユビキチンアッセイ及びスクリーニングへの利用2012

    • Inventor(s)
      村田 等、阪口政清、許南浩
    • Industrial Property Rights Holder
      村田 等、阪口政清、許南浩
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-165160
    • Filing Date
      2012-07-25
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] NPTNβとS100A8の結合の阻害を指標とする細胞増殖抑制剤のスクリーニング方法2012

    • Inventor(s)
      阪口政清、日比野利彦、山本真実、許南浩
    • Industrial Property Rights Holder
      阪口政清、日比野利彦、山本真実、許南浩
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-204279
    • Filing Date
      2012-09-18
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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