Comprehensive risk assessment of silver nanoparticles on fish molecular ecotoxicology
| Project/Area Number |
23651028
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental impact assessment/Environmental policy
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| Research Institution | Toyo University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 生態系影響評価 / ナノマテリアル / メダカ / 銀ナノ粒子 / 銀イオン / 生態リスク |
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| Research Abstract |
We investigated silver nanocolloids (SNCs) effects on gene expression with DNA microarray analyses. 118 genes were up-regulated and 117 genes were down-regulated. Since severe morphological defects and damage of central nervous system were observed in SNCs-exposed medaka embryos, we chose five genes that were relevant to vertebrate embryogenesis and/or morphogenesis and well-studied in mammalian systems. Cathepsin L (ctsl), tropomyosin 1 alpha (tpm1), and retinol-binding protein (rbp) were significantly down-regulated in SNCs exposed embryos, while sarcoplasmic / endoplasmic reticulum calcium ATPase 1 (SERCA1, atp2a1) and homeobox B6b (hoxb6b) were both up-regulated. These genes were validated with with qRT-PCR. We performed gene knockdown techniques of RNAi. We observed the deformities in medaka embryos injected with siRNA of ctsl, tpm1 or rbp.We considered the three genes are target gene candidates of SNCs.
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Report
(4 results)
Research Products
(15 results)
