Structural and functional studies on Chloroethene sensor kinase and its application for bioremediation
| Project/Area Number |
23651063
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
ODAKA Masafumi 東京農工大学, 大学院・工学研究院, 准教授 (20224248)
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| Co-Investigator(Renkei-kenkyūsha) |
YOHDA Masafumi 東京農工大学, 大学院・工学研究院, 教授 (50250105)
NOGUCHI Keiichi 東京農工大学, 学術研究支援総合センター, 准教授 (00251588)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ヒスチジンキナーゼ / PAS ドメイン / 2成分系伝達システム / クロロエテン / バイ オレメディエーション / PASドメイン / バイオレメディエーション |
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| Research Abstract |
Chloroethene is one of the most serious pollutants of soil and underground water, and the construction of its detection and removal systems is a very important problem to be solved. In this study, genes of chloroethene sensor kinase were amplified by PCR with a primers set designed based on amino acid sequences of the known chloroethene sensor kinase using a metagenome prepared from a consortium dechlorinating chloroethene to ethene as a template. The obtained sensor kinase was expressed inEscherichia coli and their structures and function were studied.
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Report
(3 results)
Research Products
(9 results)
