Development ofporous multi-layer biomaterial and basic study on regeneration of cartilage-bone multi-layer tissue

• Project/Area Number: 23656090
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Materials/Mechanics of materials
• Research Institution: Kyushu University
• Principal Investigator: TODO Mitsugu 九州大学, 応用力学研究所, 准教授 (80274538)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 生体材料 / 再生医療 / 整形外科 / 組織工学 / 関節組織 / 生体力学 / 幹細胞

Research Abstract

The primary aim of this study was to develop multi-layer scaffolds having biomechanical compatibility with natural cartilage-bone layered tissue in order to apply to regenerative medicine for large scale injuries of joints. Fundamental fabrication process for regenerated artificial bone tissue was also tried to be established. Novel bioabsorbable multi-layer porous materials imitating the mechanical properties of cartilage-bone layered tissue was successfully developed by using biodegradable polymers such as PCL and PLLA with bioceramic HA. It was found that the compressive properties of the layered scaffolds are very similar to those of natural layered tissues. Regenerated artificial bone tissues have also developed by culturing mesenchymal stem cells with organic/inorganic composite scaffold for a certain period.

Research Products

• [Journal Article] Biomechanical effect of implantation of chitosan/MWNTs reinforced scaffold into damaged femur (2013)
  • Author(s): Sunghyen Hwang and Mitsugu Todo
  • Journal Title: Journal of Novel Carbon Resource Science Volume: 7 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Compressive deformation behavior of bioabsorbable porous layered composite materials ofr articular tissue eigineering (2013)
  • Author(s): Sunghyen Hwang and Mitsugu Todo
  • Journal Title: Journal of Solid Mechanics and Materials Engineering Volume: 7(2) Pages: 1-10
  • Peer Reviewed
• [Journal Article] Development and characterization of poly(ε-caprolactone) reinforced porous hydroxyapatite for bone tissue engineering (2013)
  • Author(s): Phanny Yos and Mitsugu Todo
  • Journal Title: Key Engineering Materials Volume: 529-530 Pages: 447-452
  • Peer Reviewed
• [Journal Article] Biomechanical effect of implantation of chitosan/MWNTs reinforced scaffold into damaged femur (2013)
  • Author(s): Sunghyen Hwang, Mitsugu Todo
  • Journal Title: Journal of Novel Carbon Resource Sciences Volume: 7 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Compressive deformation behavior of bioabsorbable porous layered composite materials ofr articular tissue eigineering (2013)
  • Author(s): Sunghyen Hwang, Mitsugu Todo
  • Journal Title: Journal of Solid Mechanics and Materials Engineering Volume: 7 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Characterization of compressive deformation behavior of multi-layer porous composite materials for articular tissue engineering (2012)
  • Author(s): Sunghyen Hwang and Mitsugu Todo
  • Journal Title: Journal of Mechanical Science and Technology Volume: 26(7) Pages: 1999-2004
  • Peer Reviewed
• [Journal Article] コラーゲン/β-TCP複合系scaffoldの圧縮力学特性に及ぼす間葉系幹細胞培養の影響 (2012)
  • Author(s): 荒平高章,東藤 貢
  • Journal Title: 臨床バイオメカニクス Volume: 33 Pages: 237-244
  • NAID: 40019481020
  • Peer Reviewed
• [Journal Article] 多孔質バイオセラミックス人工骨の圧縮力学特性に及ぼすコラーゲンコーティングの影響 (2012)
  • Author(s): 荒平高章,東藤 貢
  • Journal Title: 粉体および粉末冶金 Volume: 59(7) Pages: 421-424
  • NAID: 10030992959
  • Peer Reviewed
• [Journal Article] Characterization of compressive deformation behavior of multi-layer porous composite materials for articular tissue engineering (2012)
  • Author(s): Sunghyen Hwang, Mitsugu Todo
  • Journal Title: Journal of Mechanical Science and Technology Volume: 26 Pages: 1999-2004
  • Peer Reviewed
• [Journal Article] 多孔質バイオセラミックス人工骨の圧縮力学特性に及ぼすコラーゲンコーティングの影響 (2012)
  • Author(s): 荒平高章，東藤 貢
  • Journal Title: 粉体および粉末冶金 Volume: 59 Pages: 421-424
  • NAID: 10030992959
  • Peer Reviewed
• [Journal Article] Characterization of Compressive Deformation Behavior of Multi-layer Porous Composite Materials for Articular Tissue Engineering (2012)
  • Author(s): Sunghyen Hwang and Mistugu Todo
  • Journal Title: Journal of Mechanical Science and Technology Volume: 未定
  • Peer Reviewed
• [Journal Article] Development and characterization of reinforced poly(L-lactide) scaffolds for bone tissue Engineering (2011)
  • Author(s): J.E.Park, M.Todo
  • Journal Title: Journal of Materials Science : Materials in Medicine Volume: 22 Issue: 5 Pages: 1171-1182
  • DOI: 10.1007/s10856-011-4289-4
  • Peer Reviewed
• [Presentation] 有機・無機2相多孔質構造体と間葉系幹細胞を用いた骨再生に関する基礎的研究 (2013)
  • Author(s): 東藤貢,荒平高章,名井陽
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2013-03-21
• [Presentation] 構造が異なる 2 種類の複合系足場材を用いた骨髄由来間葉系幹細胞の培養と増殖・分化・ECM形成能の比較 (2013)
  • Author(s): 東藤貢,荒平高章,名井陽
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2013-03-21
• [Presentation] 骨組織工学のための有機・無機 2 相多孔質材料の創製と評価 (2013)
  • Author(s): 荒平高章,東藤貢
  • Organizer: 第25回バイオエンジニアリング講演会
  • Place of Presentation: つくば市
  • Year and Date: 2013-01-10
• [Presentation] 骨組織工学のための有機・無機2相多孔質材料の創製と評価 (2013)
  • Author(s): 荒平高章，東藤貢
  • Organizer: 第25回バイオエンジニアリング講演会
  • Place of Presentation: 産業技術総合研究所つくばセンター
• [Presentation] コラーゲンゲル・多孔体２相scaffoldによる軟骨再生の基礎的研究 (2013)
  • Author(s): 東藤貢，中牟田侑昌
  • Organizer: 第25回バイオエンジニアリング講演会
  • Place of Presentation: 産業技術総合研究所つくばセンター
• [Presentation] β-TCP/collagen scaffold の圧縮特性に及ぼす間葉系幹細胞培養の影響 (2012)
  • Author(s): 荒平高章,東藤貢,名井陽
  • Organizer: 第39回日本臨床バイオメカニクス学会
  • Place of Presentation: 千葉市
  • Year and Date: 2012-11-10
• [Presentation] 骨組織工学用多孔質HAp/PCL複合材料の力学特性 (2012)
  • Author(s): Yos Phanny, 東藤貢
  • Organizer: 日本機械学会M&M2012材料力学カンファレンス
  • Place of Presentation: 愛媛大学
• [Presentation] コラーゲン/β-TCP複合系scaffoldを用いた間葉系幹細胞培養系における細胞外マトリックス形成機構 (2012)
  • Author(s): 東藤貢，荒平高章
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 軟骨・骨インターフェイス組織再生のための多層scaffoldの圧縮変形挙動 (2012)
  • Author(s): Hwang SungHyen，東藤貢
  • Organizer: 第25回バイオエンジニアリング講演会
  • Place of Presentation: 大阪大学（豊中市）
• [Presentation] Characterization of compressive deformation behavior of multi-layer porous composite materials for articular tissue engineering (2011)
  • Author(s): Sunghyen Hwang and Mitsugu Todo
  • Organizer: International Conference on Materials and Reliability 2011
  • Place of Presentation: Busan, Korea
  • Year and Date: 2011-11-22
• [Presentation] Development of polymer/composite multi-layer porous material for osteochondral tissue engineering (2011)
  • Author(s): Sunghyen Hwang and Mitsugu Todo
  • Organizer: The 8thKorea-Japan Joint Symposium on Composite Materials
  • Place of Presentation: Changwon, Korea.
  • Year and Date: 2011-11-17
• [Presentation] 関節組織再生のための積層構造 scaffold の圧縮変形挙動 (2011)
  • Author(s): Hwang Sunghyen,東藤貢
  • Organizer: 日本機械学会材料力学講演会(M&M2011)
  • Place of Presentation: 北九州市
  • Year and Date: 2011-07-17
• [Presentation] 軟骨・骨インターフェイス組織再生のための多層scaffold の圧縮変形挙動 (2011)
  • Author(s): Hwang Sunghyen, 東藤貢
  • Organizer: 日本機械学会第24回バイオエンジニアリング講演会
  • Place of Presentation: 大阪市
  • Year and Date: 2011-01-07
• [Presentation] 関節組織再生のための積層構造scaffoldの圧縮変形挙動 (2011)
  • Author(s): Hwang SungHyen，東藤貢
  • Organizer: M&M2011材料力学カンファレンス
  • Place of Presentation: 九州工業大学（北九州市）
• [Presentation] Development of multi-layer composite scaffold for articular regeneration (2011)
  • Author(s): SungHyen Hwang, Mitsugu Todo
  • Organizer: The 18th International Conference on Composite Materials
  • Place of Presentation: Jeju, KOREA
• [Presentation] Development of polymer/composite multi-layer porous material for osteochondral tissue engineering (2011)
  • Author(s): SungHyen Hwang, Mitsugu Todo
  • Organizer: 8th Korea-Japan Joint Symposium on Composite Materials
  • Place of Presentation: Changwon, KOREA
• [Presentation] Characterization of compressive deformation behavior of multi-layer porous composte materials for articular tissue engineering (2011)
  • Author(s): SungHyen Hwang, Mitsugu Todo
  • Organizer: International Conference on Materials and Reliability 2011
  • Place of Presentation: Busan, KOREA
• [Presentation] 有機・無機複合系多孔質材料と間葉系幹細胞を用いたin vitroでの骨組織形成
  • Author(s): 東藤貢
  • Organizer: 第16回バイオレオロジー・リサーチ・フォーラム
  • Place of Presentation: 東京大学医学部
  • Invited