| Project/Area Number |
23656530
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Tokushima |
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Principal Investigator |
OMASA Takeshi 徳島大学, 大学院・ソシオテクノサイエンス研究部, 教授 (00252586)
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| Co-Investigator(Kenkyū-buntansha) |
ONITSUKA Masayoshi 徳島大学, 大学院・ソシオテクノサイエンス研究部, 助教 (80571174)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | CHO 細胞 / 山中因子 / リプログラミング / CHO細胞 |
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| Research Abstract |
Introduction of four factors, which are called Yamanaka factors, could construct induced pluripotential stem (iPS) cells from somatic cells. In this study, we introduce these factors into Chinese hamster ovary (CHO) cells which are workhorse for biopharmaceutical production and investigated the characteristics of these CHO cells. By introducing of mouse Oct3/4, Klf4, and Sox2 factors, we constructed CHO cells which over -expressed these factors. The specific growth rate and morphology of constructed cells were not affected by introduced factors.
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