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Formation of facultative heterochromatin in fission yeast

Research Project

Project/Area Number 23657005
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Genetics/Genome dynamics
Research InstitutionKyoto University

Principal Investigator

ISHIKAWA Fuyuki  京都大学, 大学院・生命科学研究科, 教授 (30184493)

Co-Investigator(Kenkyū-buntansha) TARUMOTO Yusuke  京都大学, 大学院・生命科学研究科, 助教 (70551381)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords遺伝学 / ゲノム / 発現制御 / 発生・分化 / エピジェネティックス
Research Abstract

We have studied the molecular mechanism of facultative heterochromatin formation in fission yeast. We first demonstrated that the fission yeast meiotic genes, mei4 and ssm4 loci are heterochromatinated, as evidenced by the presence of histone H3 methylated at lysine 9 (H3K9me), in vegetative growth. This heterochromatin marker disappears upon the entry of cells into meiotic cell cycle. We then revealed that the Mmi1 -degradation machinery of mei4 and ssm4 mRNA transcript is required for the establishment of H3K9me when cells enters vegetative growth phase. Moreover, we found that DSRs, a set of mRNA regions included in mei4 and ssm4 transcripts, are required for the Mmi1 -dependent heterochromatin establishment. These results shed an insight into our understanding of how heterochromatin is formed in eukaryotes.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (62 results)

All 2013 2012 2011 Other

All Journal Article (14 results) (of which Peer Reviewed: 10 results,  Open Access: 1 results) Presentation (42 results) (of which Invited: 9 results) Book (2 results) Remarks (4 results)

  • [Journal Article] Transcription-induced chromatin association of RNA surveillance factors mediates facultative heterochromatin formation in fission yeast2013

    • Author(s)
      Tashiro, S.
    • Journal Title

      Genes to Cells

      Volume: 18 Issue: 4 Pages: 327-339

    • DOI

      10.1111/gtc.12038

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Stemness-related Factor Sall4 Interacts with Transcription Factors Oct-3/4 and Sox2 and Occupies Oct-Sox Elements in Mouse Embryonic Stem Cells.2013

    • Author(s)
      Tanimura, N., Saito, M. Ebisuya,M.,Nishida, E., and Ishikawa, F.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 288巻 Pages: 5027-5038

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stemness-related Factor Sall4 Interacts with Transcription Factors Oct-3/4 and Sox2 and Occupies Oct-Sox Elements in Mouse Embryonic Stem Cells2013

    • Author(s)
      Tanimura, N.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 288 Issue: 7 Pages: 5027-5038

    • DOI

      10.1074/jbc.m112.411173

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 新しいテロメア蛋白質複合体 CST2012

    • Author(s)
      中岡秀憲、石川冬木
    • Journal Title

      医学のあゆみ

      Volume: 241巻 Pages: 846-851

    • URL

      http://www.ishiyaku.co.jp/magazines/ayumi/AyumiArticleDetail.aspx?BC=924111&AC=11601

    • Related Report
      2012 Final Research Report
  • [Journal Article] テロメラーゼ非依存性テロメア維持機構と疾患2012

    • Author(s)
      鍋谷彰、石川冬木
    • Journal Title

      医学のあゆみ

      Volume: 241巻 Pages: 853-859

    • URL

      http://www.ishiyaku.co.jp/magazines/ayumi/AyumiArticleDetail.aspx?BC=924111&AC=11602

    • Related Report
      2012 Final Research Report
  • [Journal Article] HIRA, a conserved histone chaperone plays an essential role in low-dose stress response via transcriptional stimulation in fission yeast2012

    • Author(s)
      Chujo, M.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 287 Issue: 28 Pages: 23440-23450

    • DOI

      10.1074/jbc.m112.349944

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Kinetics of DNA replication and telomerase reaction at a single-seeded telomere in human cells2012

    • Author(s)
      Hirai, Y.
    • Journal Title

      Genes to Cells

      Volume: 17 Issue: 3 Pages: 186-204

    • DOI

      10.1111/j.1365-2443.2012.01581.x

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tel1ATM and Rad3ATR phosphorylate the telomere protein Ccq1 to recruit telomerase and elongate telomeres in fission yeast2012

    • Author(s)
      Yamazaki, H.
    • Journal Title

      Genes & Development

      Volume: 26 Issue: 3 Pages: 241-246

    • DOI

      10.1101/gad.177873.111

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Xenopus laevis Ctc1-Stn1-Ten1 (xCST) complex is involved in priming DNA synthesis on single-stranded DNA template in Xenopus egg extract2012

    • Author(s)
      Nakaoka, H.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 287 Issue: 1 Pages: 619-627

    • DOI

      10.1074/jbc.m111.263723

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 新しいテロメア蛋白質複合体CST2012

    • Author(s)
      中岡 秀憲
    • Journal Title

      医学のあゆみ

      Volume: 241 Pages: 846-851

    • Related Report
      2012 Annual Research Report
  • [Journal Article] テロメラーゼ非依存性テロメア維持機構と疾患2012

    • Author(s)
      鍋谷 彰
    • Journal Title

      医学のあゆみ

      Volume: 241 Pages: 853-859

    • Related Report
      2012 Annual Research Report
  • [Journal Article] テロメア異常と発癌2012

    • Author(s)
      石川 冬木
    • Journal Title

      臨床血液

      Volume: 53 Pages: 365-372

    • NAID

      10031123876

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Essential roles of Xenopus TRF2in telomere end protection and replication.2011

    • Author(s)
      Muraki, K., Nabetani, A.,Nishiyama, A., and Ishikawa, F
    • Journal Title

      Genes to Cells

      Volume: 16巻 Pages: 728-739

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Essential roles of Xenopus TRF2 in telomere end protection and replication2011

    • Author(s)
      Muraki, K.
    • Journal Title

      Genes to Cells

      Volume: 16 Issue: 6 Pages: 728-739

    • DOI

      10.1111/j.1365-2443.2011.01520.x

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 老化分子機構の多義性2013

    • Author(s)
      石川冬木
    • Organizer
      国際高等研究所第2回「老いを考える」研究会
    • Place of Presentation
      京都府、国際高等研究所
    • Year and Date
      2013-02-02
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞老化における NF-kappaB経路の役割」2012

    • Author(s)
      石川冬木
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡県、福岡国際会議場・マリンメッセ福岡
    • Year and Date
      2012-12-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Chromosomal rearrangements in fission yeast with dysfunctional telomeres2012

    • Author(s)
      Fuyuki Ishikawa
    • Organizer
      日仏がんワークショップ
    • Place of Presentation
      徳島県、グランドエクシブ鳴門
    • Year and Date
      2012-11-30
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 高校生のためのがん研究入門2012

    • Author(s)
      石川冬木
    • Organizer
      がん研究の現在・未来
    • Place of Presentation
      石川県、金沢歌劇座
    • Year and Date
      2012-11-17
    • Related Report
      2012 Final Research Report
  • [Presentation] CST Complex in Telomere DNA Replication2012

    • Author(s)
      石川冬木
    • Organizer
      Keystone Symposia Aging and Diseases of Aging (S2)
    • Place of Presentation
      東京都、シェラトン都ホテル東京
    • Year and Date
      2012-10-25
    • Related Report
      2012 Final Research Report
  • [Presentation] テロメアと発癌2012

    • Author(s)
      石川冬木
    • Organizer
      第74回日本血液学会学術集会
    • Place of Presentation
      京都市、国立京都国際会館
    • Year and Date
      2012-10-19
    • Related Report
      2012 Final Research Report
  • [Presentation] がん細胞を標的にする2012

    • Author(s)
      石川冬木
    • Organizer
      第20回日本癌学会市民公開講座「がん先端研究とがん治療の現在・将来」
    • Place of Presentation
      北海道、札幌市民ホール
    • Year and Date
      2012-09-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Telomere dysfunctions and chromosome instability2012

    • Author(s)
      Fuyuki Ishikawa
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌市、ロイトン札幌
    • Year and Date
      2012-09-20
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] テロメアの複製と DNA 組換え2012

    • Author(s)
      石川冬木
    • Organizer
      第14回生命科学研究科シンポジウム
    • Place of Presentation
      京都市、京都大学芝蘭会館
    • Year and Date
      2012-07-11
    • Related Report
      2012 Final Research Report
  • [Presentation] テロメレースによるテロメア DNA 伸長機構2012

    • Author(s)
      石川冬木
    • Organizer
      第49回日本臨床分子医学会学術集会
    • Place of Presentation
      京都市、みやこめっせ
    • Year and Date
      2012-04-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Conserved molecular architectures of telomere chromatin in eukaryotes2011

    • Author(s)
      Fuyuki Ishikawa
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜市、パシフィコ横浜
    • Year and Date
      2011-12-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Molecular mechanisms of controlingtelomerase reaction in fission yeast2011

    • Author(s)
      Fuyuki Ishikawa
    • Organizer
      日仏がんワークショップ
    • Place of Presentation
      Mercure Hotel, Montpellier, France
    • Year and Date
      2011-11-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Highly conserved molecular architectures of telomeres in eukaryotes2011

    • Author(s)
      Fuyuki Ishikawa
    • Organizer
      日米がん研究協力事業ワークショップ
    • Place of Presentation
      京都市、京都ホテルオークラ
    • Year and Date
      2011-10-25
    • Related Report
      2012 Final Research Report
  • [Presentation] Telomere Dysfunction and GeneticInstability2011

    • Author(s)
      Fuyuki Ishikawa
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋市、名古屋国際会議場
    • Year and Date
      2011-10-04
    • Related Report
      2012 Final Research Report
  • [Presentation] テロメアの維持機構とテロメア短小化症候群2011

    • Author(s)
      石川冬木
    • Organizer
      第14回間質性肺炎細胞分子病態研究会
    • Place of Presentation
      東京都、シェーンバッハ・サボー(砂防会館)
    • Year and Date
      2011-08-20
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒストン修飾 mRNA 分解2011

    • Author(s)
      石川冬木
    • Organizer
      第13回生命科学研究科シンポジウム
    • Place of Presentation
      京都市、京都大学芝蘭会館
    • Year and Date
      2011-07-07
    • Related Report
      2012 Final Research Report
  • [Presentation] mRNA elimination system directs the formation of facultative heterochromatin in fission yeast2011

    • Author(s)
      Sanki Tashiro, Tomohiro Asano, Junko Kanoh, and Fuyuki Ishikawa
    • Organizer
      The 6th International Fission Yeast Meeting
    • Place of Presentation
      Simmons Main Campus Building, USA
    • Year and Date
      2011-06-28
    • Related Report
      2012 Final Research Report
  • [Presentation] How do telomeres sense their length?2011

    • Author(s)
      Fuyuki Ishikawa
    • Organizer
      第9回京都大学・国立台湾大学合同「分子細胞生物学シンポジウム」
    • Place of Presentation
      京都市、京都大学吉田構内 総合人間学部棟
    • Year and Date
      2011-06-04
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞老化:持続的炎症がもたらす生体防御反応2011

    • Author(s)
      石川冬木
    • Organizer
      第11回日本抗加齢医学会総会
    • Place of Presentation
      京都市、国立京都国際会館
    • Year and Date
      2011-05-27
    • Related Report
      2012 Final Research Report
  • [Presentation] Thesequence of events at a single humantelomere in S phase2011

    • Author(s)
      Yugo Hirai, Michihito Wakai, KenkichiMasutomi, and Fuyuki Ishikawa
    • Organizer
      Telomeres andTelomerase
    • Place of Presentation
      Cold SpringHarbor Laboratory, Cold Spring Harbor, NewYork, USA
    • Year and Date
      2011-05-03
    • Related Report
      2012 Final Research Report
  • [Presentation] The sequence of events at a single human telomere in S phase.2011

    • Author(s)
      Yugo Hirai, Michihito Wakai, Kenkichi Masutomi, and Fuyuki Ishikawa
    • Organizer
      Telomeres and Telomerase(招待講演)
    • Place of Presentation
      Cold Spring Harbor Laboratory (USA)
    • Related Report
      2011 Research-status Report
  • [Presentation] 細胞老化:持続的炎症がもたらす生体防御反応2011

    • Author(s)
      石川冬木
    • Organizer
      第11回日本抗加齢医学会総会(招待講演)
    • Place of Presentation
      国立京都国際会館(京都府)
    • Related Report
      2011 Research-status Report
  • [Presentation] How do telomeres sense their length?2011

    • Author(s)
      Fuyuki Ishikawa
    • Organizer
      第9回京都大学・国立台湾大学合同「分子細胞生物学シンポジウム」(招待講演)
    • Place of Presentation
      京都大学吉田構内 総合人間学部棟(京都府)
    • Related Report
      2011 Research-status Report
  • [Presentation] ヒストン修飾mRNA分解2011

    • Author(s)
      石川冬木
    • Organizer
      第13回生命科学研究科シンポジウム
    • Place of Presentation
      京都大学芝蘭会館(京都府)
    • Related Report
      2011 Research-status Report
  • [Presentation] テロメアの維持機構とテロメア短小化症候群2011

    • Author(s)
      石川冬木
    • Organizer
      第14回間質性肺炎細胞分子病態研究会(招待講演)
    • Place of Presentation
      シェーンバッハ・サボー(砂防会館)(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] Telomere Dysfunction and Genetic Instability.2011

    • Author(s)
      Fuyuki Ishikawa
    • Organizer
      第70回日本癌学会学術総会(招待講演)
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Highly conserved molecular architectures of telomeres in eukaryotes.2011

    • Author(s)
      Fuyuki Ishikawa
    • Organizer
      日米がん研究協力事業ワークショップ(招待講演)
    • Place of Presentation
      京都ホテルオークラ(京都府)
    • Related Report
      2011 Research-status Report
  • [Presentation] Molecular mechanisms of controling telomerase reaction in fission yeast.2011

    • Author(s)
      Fuyuki Ishikawa
    • Organizer
      日仏がんワークショップ(招待講演)
    • Place of Presentation
      Mercure Hotel, Montpellier (France)
    • Related Report
      2011 Research-status Report
  • [Presentation] Conserved molecular architectures of telomere chromatin in eukaryotes.2011

    • Author(s)
      Fuyuki Ishikawa
    • Organizer
      第34回日本分子生物学会年会(招待講演)
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] mRNA elimination system directs the formation of facultative heterochromatin in fission yeast.2011

    • Author(s)
      Sanki Tashiro, Tomohiro Asano, Junko Kanoh, and Fuyuki Ishikawa
    • Organizer
      The 6th International Fission Yeast Meeting
    • Place of Presentation
      Simmons Main Campus Building (USA)
    • Related Report
      2011 Research-status Report
  • [Presentation] テロメアと染色体組換え

    • Author(s)
      石川冬木
    • Organizer
      平成 24 年度「がん研究分野の特性等を踏まえた支援活動」公開シンポジウム
    • Place of Presentation
      東京都、学術総合センター 一橋記念講堂
    • Related Report
      2012 Final Research Report
  • [Presentation] テロメアの生化学:テロメアは DNA 損傷チェックポイント分子が好きか嫌いか?

    • Author(s)
      石川冬木
    • Organizer
      生化学会北陸支部集会
    • Place of Presentation
      金沢市、金沢歌劇座
    • Related Report
      2012 Final Research Report
  • [Presentation] テロメレースによるテロメアDNA伸長機構

    • Author(s)
      石川 冬木
    • Organizer
      第49回日本臨床分子医学会学術集会
    • Place of Presentation
      京都市、みやこめっせ
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] テロメアの生化学:テロメアはDNA損傷チェックポイント分子が好きか嫌いか?

    • Author(s)
      石川 冬木
    • Organizer
      生化学会北陸支部集会
    • Place of Presentation
      金沢市、金沢歌劇座
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] テロメアの複製とDNA組換え

    • Author(s)
      石川 冬木
    • Organizer
      第14回生命科学研究科シンポジウム
    • Place of Presentation
      京都市、京都大学芝蘭会館
    • Related Report
      2012 Annual Research Report
  • [Presentation] がん細胞を標的にする

    • Author(s)
      石川 冬木
    • Organizer
      第20回日本癌学会市民公開講座「がん先端研究とがん治療の現在・将来」
    • Place of Presentation
      北海道、札幌市民ホール
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] テロメアと発癌

    • Author(s)
      石川 冬木
    • Organizer
      第74回日本血液学会学術集会
    • Place of Presentation
      京都市、国立京都国際会館
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] CST Complex in Telomere DNA Replication

    • Author(s)
      石川 冬木
    • Organizer
      Keystone Symposia Aging and Diseases of Aging (S2)
    • Place of Presentation
      東京都、シェラトン都ホテル東京
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 高校生のためのがん研究入門

    • Author(s)
      石川 冬木
    • Organizer
      がん研究の現在・未来
    • Place of Presentation
      石川県、金沢歌劇座
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 細胞老化におけるNF-kappaB経路の役割

    • Author(s)
      石川 冬木
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡県、福岡国際会議場・マリンメッセ福岡
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] テロメアと染色体組換え

    • Author(s)
      石川 冬木
    • Organizer
      平成24年度「がん研究分野の特性等を踏まえた支援活動」公開シンポジウム
    • Place of Presentation
      東京都、学術総合センター 一橋記念講堂
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 老化分子機構の多義性

    • Author(s)
      石川 冬木
    • Organizer
      国際高等研究所第2回「老いを考える」研究会
    • Place of Presentation
      京都府、国際高等研究所
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] デヴィータがんの分子生物学2012

    • Author(s)
      石川冬木(分担翻訳) 宮園浩平、石川冬木、間野博行監訳
    • Total Pages
      544
    • Publisher
      メディカル・サイエンス・インターナショナル
    • Related Report
      2012 Final Research Report
  • [Book] メディカル・サイエンス・インターナショナル2012

    • Author(s)
      石川冬木(分担翻訳) 宮園浩平、石川冬木、間野博行監訳
    • Total Pages
      544
    • Publisher
      デヴィータがんの分子生物学
    • Related Report
      2012 Annual Research Report
  • [Remarks] 石川研究室ホームページ

    • URL

      http://www.lif.kyoto-u.ac.jp/labs/fish/

    • Related Report
      2012 Final Research Report
  • [Remarks] 京都大学大学院生命科学研究科ホームページ

    • URL

      http://www.lif.kyoto-u.ac.jp/j/

    • Related Report
      2012 Final Research Report
  • [Remarks] 石川研究室ホームページ

    • URL

      http://www.lif.kyoto-u.ac.jp/labs/fish/

    • Related Report
      2012 Annual Research Report
  • [Remarks] 京都大学生命科学研究科ホームページ

    • URL

      http://www.lif.kyoto-u.ac.jp/j/

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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