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Studies on the formation process of symbiotic microbial community using host hybridization technique

Research Project

Project/Area Number 23657013
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Ecology/Environment
Research InstitutionIbaraki University

Principal Investigator

KITADE Osamu  茨城大学, 理学部, 准教授 (80302321)

Co-Investigator(Kenkyū-buntansha) NODA Satoko  山梨大学, 総合研究部, 准教授 (80342830)
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords共生 / 原生生物 / 群集 / 群集構造 / 群集の混合 / 大規模移入 / 異種間交雑 / 社会性昆虫 / 多様性
Outline of Final Research Achievements

To elucidate characteristics of microbial community in its formation process, we crossed three termite species harboring species-specific symbiotic microbial compositions, and examined community structure of newly formed symbiont community of their hybrids. In most cases symbiont compositions in the hybrid termites converged to a stable composition of a particular parent species. Antibiotic treatment had significant negative effects on the establishment of symbiotic protist compositions of the hybrid colonies. These results probably reflect long-term complex co-adaptaion between symbiotic microorganisms. We also clarified effects of temperature on the sybiotic protist community structures and protist faunae of the field colonies of two host termite species.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (29 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 7 results,  Open Access: 3 results) Presentation (17 results) Book (3 results)

  • [Journal Article] Distribution and Evolution of Nitrogen Fixation Genes in the Phylum <i>Bacteroidetes</i>2015

    • Author(s)
      Inoue, J., Oshima K., Suda, W., Sakamoto, M., Iino, T., Noda, S., Hongoh, Y., Hattori, M. and Ohkuma, M.
    • Journal Title

      Microbes and Environments

      Volume: 30 Issue: 1 Pages: 44-50

    • DOI

      10.1264/jsme2.ME14142

    • NAID

      130005061711

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intranuclear verrucomicrobial symbionts and implication of lateral gene transfer to the host protist in the termite gut.2014

    • Author(s)
      Sato, T., Kuwahara, H., Fujita, K., Noda, S., Kihara, K., Yamada, A., Ohkuma, M. and Hongoh, Y.
    • Journal Title

      ISME journal

      Volume: 8 Issue: 5 Pages: 1008-1019

    • DOI

      10.1038/ismej.2013.222

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intracolonial genetic variation affects reproductive skew and colony productivity during colony foundation in a parthenogenetic termite2014

    • Author(s)
      Satoshi Miyazaki, Miho Yoshimura, Ryota Saiki, Yoshinobu Hayashi, Osamu Kitade, Kazuki Tsuji, Kiyoto Maekawa
    • Journal Title

      BMC Evolutionary Biology

      Volume: 14 Issue: 1 Pages: 177-177

    • DOI

      10.1186/s12862-014-0177-0

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intranuclear verrucomicrobial symbionts and implication of lateral gene transfer to the host protist in the termite gut2014

    • Author(s)
      T.Sato, H. Kuwahara, K. Fujita, S. Noda, K. Kihara, A. Yamada, M. Ohkuma, Y.Hongoh
    • Journal Title

      ISME journal

      Volume: 8 Pages: 1008-1009

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] “メタトランスクリプトーム研究の現状”常在細菌叢が操る ヒトの健康と疾患,2014

    • Author(s)
      野田悟子,大熊盛也
    • Journal Title

      実験医学増刊号

      Volume: 32 Pages: 693-698

    • Related Report
      2013 Research-status Report
  • [Journal Article] Symbiotic protist communities in the termite Coptotermes formosanus in Japan and a comparison of community structures between workers and soldiers2013

    • Author(s)
      Kitade, O., Hayashi, Y., Noda, S.
    • Journal Title

      Japanese Journal of Protozoology

      Volume: 46 Pages: 21-29

    • NAID

      130005254719

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Variation and diversity of symbiotic protist composition in the damp-wood termite Hodotermopsis sjoestedti.2012

    • Author(s)
      O. Kitade, Y. Hayashi, K. Takatsuto, T. Matsumoto
    • Journal Title

      Jap. J. Protozool.

      Volume: 45 Pages: 29-36

    • NAID

      130005254718

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 環境微生物のオミックス解析2012

    • Author(s)
      野田悟子
    • Journal Title

      生物工学会誌

      Volume: 90 Pages: 654-654

    • NAID

      110009553242

    • Related Report
      2012 Research-status Report
  • [Journal Article] Molecular phylogeny and evolution of Parabasalia with improved taxon sampling and new protein markers of actin and elongation factor-12011

    • Author(s)
      Noda, S., Mantini, C., Meloni, D., Inoue, J.-L., Kitade, O,, Viscogliosi, E., Ohkuma, M.
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 1 Pages: e29938-e29938

    • DOI

      10.1371/journal.pone.0029938

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 他種間のシロアリにおける腸内原生生物の交換にバクテリアが与える影響2015

    • Author(s)
      北出理,池内康介
    • Organizer
      日本生態学会第62回大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヤマトシロアリにおける初期コロニーと成熟コロニーの相互作用2015

    • Author(s)
      北出理,竹内智勇
    • Organizer
      日本生態学会第62回大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dynamics of symbiotic protist community after community mixing through the hybridization of two host termite species.2014

    • Author(s)
      O. Kitade
    • Organizer
      2nd Asian congress of protistology
    • Place of Presentation
      Kalyani, India
    • Year and Date
      2014-11-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] シロアリ腸内原生生物が有する糖質加水分解酵素の多型解析2014

    • Author(s)
      米山真吾,行仕圭祐,天野公貴,雪真弘,飯田敏也,大熊盛也,宇井定春,野田悟子
    • Organizer
      日本微生物生態学会第29回大会
    • Place of Presentation
      浜松
    • Year and Date
      2014-10-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Genetic diversity of cellulolytic enzymes in the termite-gut protists2014

    • Author(s)
      S. Noda, M. Yuki, T. Iida, K. Gyoji, K. Amano, S. Suzuki, M. Ohkuma, S. Ui
    • Organizer
      International symposium on microbial ecology
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2014-08-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Molecular phylogeny and evolution of Parabasalia with new protein markers and improved taxon sampling2012

    • Author(s)
      S. Noda, Eric Viscogliosi, M. Ohkuma
    • Organizer
      International symposium on microbial ecology
    • Place of Presentation
      Copenhagen, Denmark
    • Related Report
      2012 Research-status Report
  • [Presentation] ヤマトシロアリの共生原生生物群集に対する温度の影響2012

    • Author(s)
      根本まなみ, 北出理
    • Organizer
      日本生態学会第59回大会・第5回東アジア生態学会連合大会
    • Place of Presentation
      龍谷大学(滋賀)
    • Related Report
      2011 Research-status Report
  • [Presentation] オオシロアリ野外巣のカスト組成・性比と共生原生生物組成2012

    • Author(s)
      北出理, 高津戸香
    • Organizer
      第56回日本応用動物昆虫学会大会
    • Place of Presentation
      近畿大学(奈良)
    • Related Report
      2011 Research-status Report
  • [Presentation] シロアリの共生Spirotrichonymphea綱原生生物の分子系統解析2011

    • Author(s)
      高柳美希, 北出理,大槻隆司,宇井定春,大熊盛也,野田悟子
    • Organizer
      第27回日本微生物生態学会
    • Place of Presentation
      京都大学(京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 腸内原生生物群集における群集混合後の種組成の変化

    • Author(s)
      北出 理,來栖 嘉宏
    • Organizer
      日本原生動物学会第46回大会
    • Place of Presentation
      西条
    • Related Report
      2013 Research-status Report
  • [Presentation] シロアリ腸内原生生物核内共生細菌の同定と宿主への遺伝子水平伝播

    • Author(s)
      藤田一磨, 佐藤朋之, 桑原宏和, 野田悟子, 大熊盛也, 本郷裕一
    • Organizer
      日本微生物生態学会 年次大会2013
    • Place of Presentation
      鹿児島
    • Related Report
      2013 Research-status Report
  • [Presentation] 「ニンフ遺伝子型」卵の導入がヤマトシロアリの初期コロニーに与える影響

    • Author(s)
      北出理, 髙津戸香, 滋田友恒
    • Organizer
      日本生態学会第61回大会
    • Place of Presentation
      広島
    • Related Report
      2013 Research-status Report
  • [Presentation] ヤマトシロアリの単為・有性初期コロニーの特性~利他性と適応度の関係~

    • Author(s)
      高津戸香,北出理
    • Organizer
      日本生態学会第61回大会
    • Place of Presentation
      広島
    • Related Report
      2013 Research-status Report
  • [Presentation] ヤマトシロアリの初期コロニ―におけるコロニー間相互作用

    • Author(s)
      矢吹健太,北出理
    • Organizer
      日本生態学会第61回大会
    • Place of Presentation
      広島
    • Related Report
      2013 Research-status Report
  • [Presentation] イエシロアリの共生原生生物群集の種組成と群集構造

    • Author(s)
      北出 理
    • Organizer
      第45回日本原生動物学会大会
    • Place of Presentation
      姫路
    • Related Report
      2012 Research-status Report
  • [Presentation] シロアリ3種の雑種コロニーにおける共生原生生物群集の変化

    • Author(s)
      北出 理、來栖 嘉宏
    • Organizer
      第60回日本生態学会大会
    • Place of Presentation
      静岡
    • Related Report
      2012 Research-status Report
  • [Presentation] 食材性昆虫に共生する原生生物の木質分解酵素遺伝子の多型解析

    • Author(s)
      行仕圭佑、雪 真弘、大槻隆司、大熊盛也、宇井定春、野田悟子
    • Organizer
      日本微生物生態学会
    • Place of Presentation
      豊橋
    • Related Report
      2012 Research-status Report
  • [Book] “昆虫と共生する原生生物”  環境と微生物の辞典2014

    • Author(s)
      野田悟子
    • Total Pages
      448
    • Publisher
      朝倉書店
    • Related Report
      2014 Annual Research Report
  • [Book] 昆虫に共生する原生生物,環境と微生物の事典(日本微生物生態学会編)2014

    • Author(s)
      野田悟子
    • Total Pages
      448
    • Publisher
      朝倉書店
    • Related Report
      2013 Research-status Report
  • [Book] シロアリ,行動生物学事典(上田恵介他編)2013

    • Author(s)
      北出 理
    • Total Pages
      637
    • Publisher
      東京化学同人
    • Related Report
      2013 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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