Investigation of color tuning mechanism in rhodopsins and production of multi-colored pigments

• Project/Area Number: 23657100
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Nagoya University
• Principal Investigator: SUDO Yuki 名古屋大学, 理学(系)研究科(研究院), 准教授 (10452202)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Shigehiko 京都大学, 大学院・理学研究科, 教授 (70402758)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 色 / タンパク質 / 光 / 実験と理論の融合 / 生物物理 / シグナル伝達 / 生体分子 / 分子認識 / 分子機械 / 超分子化学 / ナノバイオ / ロドプシン

Research Abstract

Rhodopsins are known to show a large variation in their colors depending on the interaction between the apoprotein (opsin) and the retinal chromophore. In this project, we have investigated the color tuning mechanism in the rhodopsins and have also produced molecules showing a variety of colors such as blue, orange, red and purple, without loss of biological function. Thus the combination of experimental and theoretical studies could provide a useful research tool in a number of scientific fields.

Research Products

• [Journal Article] Color discriminating retinal configurations of sensory rhodopsin I by photo-irradiation solid state NMR spectroscopy (2014)
  • Author(s): Yomoda, H., Makino, Y., Tomonaga, Y., Hidaka, T., Kawamura, I., Okitsu, T., Wada, A., Sudo, Y., & Naito, A
  • Journal Title: Angew. Chem. Int. Ed Volume: (In press)
• [Journal Article] The early steps in the photocycle of a photosensor protein sensory rhodopsin I from Salinibacter ruber (2014)
  • Author(s): Sudo, Y., Mizuno, M., Wei, Z., Takeuchi, S., Tahara, T., & Mizutani, Y
  • Journal Title: J. Phys. Chem. B Volume: 118 Pages: 1510-1518
• [Journal Article] Color discriminating retinal configurations of sensory rhodopsin I by photo-irradiation solid state NMR spectroscopy. (2014)
  • Author(s): Yornoda, H., Makino, Y., Tomonaga, Y., Hidaka, T., Kawamura, I., Okitsu, T., Wada, A., Sudo, Y., Naito, A.
  • Journal Title: Angew. Chem. Int. Ed. Volume: (印刷中) Issue: 27 Pages: 7080-7084
  • DOI: 10.1002/ange.201309258
  • Peer Reviewed
• [Journal Article] Thermal and spectroscopic characterization of a proton pumping rhodopsin from an extreme thermophile (2013)
  • Author(s): Tsukamoto, T., Inoue, K., Kandori, H., & Sudo, Y
  • Journal Title: J. Biol. Chem Volume: 288 Pages: 21581-21592
• [Journal Article] A blue-shifted light-driven proton pump for neural silencing (2013)
  • Author(s): Sudo, Y., Okazaki, A., Ono, H., Yagasaki, J., Sugo, S., Kamiya, M., Reissig, L., Inoue, K., Ihara, K., Kandori, H., Takagi, S., & Hayashi, S
  • Journal Title: J. Biol. Chem Volume: 288 Pages: 20624-20632
• [Journal Article] Investigation of the chromophore binding cavity in the 11-cis acceptable microbial rhodopsin MR (2013)
  • Author(s): Mori, A., Yagasaki, J., Homma, M., Reissig, L., & Sudo, Y
  • Journal Title: Chem. Phys Volume: 419 Pages: 23-29
• [Journal Article] Large spectral change due to amide modes of a β-sheet upon the formation of an early photointermediate of middle rhodopsin (2013)
  • Author(s): Furutani, Y., Okitsu, T., Reissig, L., Mizuno, M., Homma, M., Wada, A., Mizutani, Y., & Sudo, Y
  • Journal Title: J. Phys. Chem. B Volume: 117 Pages: 3449-3458
• [Journal Article] Thermal and spectroscopic characterization of a proton pumping rhodopsin from an extreme thermophile (2013)
  • Author(s): Tsukamoto, T., Inoue, K., Kandori, H., Sudo, Y.
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 30 Pages: 21581-21592
  • DOI: 10.1074/jbc.m113.479394
  • Peer Reviewed
• [Journal Article] A blue-shifted light-driven proton pump for neural silencing (2013)
  • Author(s): Sudo, Y., Okazaki, A., Ono, H., Yagasaki, J., Sugo, S., Kamiya, M., Reissig, L., Inoue, K., Ihara, K., Kandori, H., Takagi, S., Hayashi, S.
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 28 Pages: 20624-20632
  • DOI: 10.1074/jbc.m113.475533
  • Peer Reviewed
• [Journal Article] Investigation of the chromophore binding cavity in the 11-cis acceptable microbial rhodopsin MR (2013)
  • Author(s): Mori, A., Yagasaki, J., Homma, M., Reissig, L., & *Sudo, Y.
  • Journal Title: Chem. Phys. Volume: 419 Pages: 23-29
  • DOI: 10.1016/j.chemphys.2012.11.020
  • Peer Reviewed
• [Journal Article] ATP hydrolysis mechanism in kinesin studied by combined quantum-mechanical molecular-mechanical metadynamics (2013)
  • Author(s): M. J. McGrath、I-F. W. Kuo, S.Hayashi, S. Takada
  • Journal Title: J. Amer. Chem. Soc. Volume: (in press) Issue: 24 Pages: 8908-891
  • DOI: 10.1021/ja401540g
  • Peer Reviewed
• [Journal Article] Expression, purification and biochemical characterization of the cytoplasmic loop of PomA, a stator component of the Na⁺ driven flagellar motor (2013)
  • Author(s): Abe R
  • Journal Title: BIOPHYSICS Volume: 9 Issue: 0 Pages: 21-29
  • DOI: 10.2142/biophysics.9.21
  • NAID: 130003366026
  • ISSN: 1349-2942
  • Peer Reviewed
• [Journal Article] The influence of halide binding on the hydrogen bonding network in the active site of Salinibacter sensory rhodopsin I (2012)
  • Author(s): Reissig, L., Iwata, T., Kikukawa, T., Demura, M., Kamo, N., Kandori, H., & Sudo, Y
  • Journal Title: Biochemistry Volume: 51 Pages: 8802-8813
• [Journal Article] Photo-induced regulation of the chromatic adaptive gene expression by Anabaena sensory rhodopsin (2012)
  • Author(s): Irieda, H., Morita, T., Maki, K., Homma, M., Aiba, H., & Sudo, Y
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 32485-32493
• [Journal Article] Absorption spectra and photochemical reactions in a unique photoactive protein, middle rhodopsin MR (2012)
  • Author(s): Inoue, K., Reissig, L., Sakai, M., Kobayashi, S., Homma, M., Fujii, M., Kandori, H., & Sudo, Y
  • Journal Title: J. Phys. Chem. B Volume: 116 Pages: 5888-5899
• [Journal Article] Crystal structure of the channelrhodopsin light-gated cation channel (2012)
  • Author(s): Kato HE, Zhang F, Yizhar O, Ramakrishnan C, Nishizawa T, Hirata K, Ito J, Aita Y, Tsukazaki T, Hayashi S, Hegemann P, Maturana AD, Ishitani R, Deisseroth K, Nureki O
  • Journal Title: Nature Volume: 482 Pages: 369-374
• [Journal Article] Photo-induced regulation of the chromatic adaptive gene expression by Anabaena sensory rhodopsin (2012)
  • Author(s): Irieda, H., Morita, T., Maki, K., Homma, M., Aiba, H., & *Sudo, Y.
  • Journal Title: J. Biol. Chem. Volume: 287 Issue: 39 Pages: 32485-32493
  • DOI: 10.1074/jbc.m112.390864
  • Peer Reviewed
• [Journal Article] Optical Silencing of C. elegans Cells with Arch Proton Pump (2012)
  • Author(s): Okazaki A, Sudo Y, Takagi S
  • Journal Title: PLoS ONE Volume: 7(5) Issue: 5 Pages: e35370-e35370
  • DOI: 10.1371/journal.pone.0035370
  • Peer Reviewed
• [Journal Article] Spectral tuning in sensory rhodopsin I from Salinibacter ruber (2011)
  • Author(s): Sudo, Y., Yuasa, Y., Shibata, J., Suzuki, D., & Homma, M
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 11328-11336
• [Journal Article] A microbial rhodopsin with a unique retinal composition shows both sensory rhodopsin II and bacteriorhodopsin-like properties (2011)
  • Author(s): Sudo, Y., Ihara, K., Kobayashi, S., Suzuki, D., Irieda, H., Kikukawa, T., Kandori, H., & Homma, M
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 5967-5976
• [Journal Article] Structural characteristics around the b-Ionone ring of the retinal chromophore in Salinibacter sensory rhodopsin I (2011)
  • Author(s): Irieda, H., Reissig, L., Kawanabe, A., Homma, M., Kandori, H., & Sudo, Y
  • Journal Title: Biochemistry Volume: 50 Pages: 4912-4922
• [Journal Article] Functional expression of a two-transmembrane HtrII protein using cell-free synthesis (2011)
  • Author(s): Sudo, Y., Tanaka, R., Kobayashi, T., Kamo, N., Kohno, T., & Kojima, C
  • Journal Title: Biophysics Volume: 7 Pages: 51-58
  • NAID: 130000741389
• [Journal Article] Spectral tuning in sensory rhodopsin I from Salinibacter ruber (2011)
  • Author(s): Sudo, Y., Yuasa, Y., Shibata, J., Suzuki, D., Homma, M.
  • Journal Title: J.Biol.Chem. Volume: 286 Issue: 13 Pages: 11328-11336
  • DOI: 10.1074/jbc.m110.187948
  • Peer Reviewed
• [Journal Article] Direct observation of the structural change of Tyr174 in the primary reaction of sensory rhodopsin II (2011)
  • Author(s): Misao Mizuno, Yuki Sudo, Michio Homma and Yasuhisa Mizutani
  • Journal Title: Biochemistry Volume: 50 Issue: 15 Pages: 3170-3180
  • DOI: 10.1021/bi101817y
  • Peer Reviewed
• [Journal Article] Spectrally silent intermediates during the photochemical reactions of Salinibacter sensory rhodopsin I (2011)
  • Author(s): K.Inoue, Y.Sudo, M.Homma, H.Kandori
  • Journal Title: J.Phys.Chem.B Volume: 115 Issue: 15 Pages: 4500-4508
  • DOI: 10.1021/jp2000706
  • Peer Reviewed
• [Journal Article] Structural characteristics around β-ionone ring of the retinal chromophore in Salinibacter sensory rhodopsin I (2011)
  • Author(s): H.Irieda, L.Reissig, A.Kawanabe, M.Homma, H.Kandori, Y.Sudo
  • Journal Title: Biochemistry Volume: 50 Issue: 22 Pages: 4912-4922
  • DOI: 10.1021/bi200284s
  • Peer Reviewed
• [Journal Article] An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy (2011)
  • Author(s): Tomonaga, Y., Hidaka, T, Kawamura, I, Nishio, T, Ohsawa, K, Okitsu, T, Wada, A, Sudo, Y, Kamo, N, Ramamoorthy, A, Naito, A.
  • Journal Title: Biophys.J. Volume: 101 Issue: 10 Pages: L50-L52
  • DOI: 10.1016/j.bpj.2011.10.022
  • Peer Reviewed
• [Journal Article] A theoretical study on excited state double proton transfer reaction of a 7-azaindole dimer: an ab initio potential energy surface and its empirical valence bond model (2011)
  • Author(s): K. Ando, S. Hayashi, S. Kato
  • Journal Title: Physical Chemistry Chemical Physics Volume: 13 Issue: 23 Pages: 11118-11127
  • DOI: 10.1039/c1cp20420c
  • Peer Reviewed
• [Presentation] Photobiophysical chemistry : What should we learn from retinal proteins? (2014)
  • Author(s): Sudo, Y
  • Organizer: iCeMS symposium on Mesoscopic Chemical Biology : Integrated Chemical-Physical Systems Towards Cell Control
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2014-02-02
• [Presentation] Photobiophysical chemistry: What should we learn from retinal proteins? (2014)
  • Author(s): Sudo, Y.
  • Organizer: iCeMS symposium on Mesoscopic Chemical Biology: Integrated Chemical-Physical Systems Towards Cell Control
  • Place of Presentation: Kyoto
  • Invited
• [Presentation] 微生物型レチナールタンパク質の非常識で未来を拓く (2013)
  • Author(s): 須藤雄気
  • Organizer: 分子科学研究所研究会「ロドプシン研究の故きを温ねて新しきを知る」
  • Place of Presentation: 岡崎
  • Invited
• [Presentation] 光受容レチナールタンパク質の常識と非常識 (2013)
  • Author(s): 須藤雄気
  • Organizer: 東京工業大学第1回生体分子専攻・若手コロキウム
  • Place of Presentation: 横浜
  • Invited
• [Presentation] 微生物型レチナールタンパク質の理解と利用 (2013)
  • Author(s): 須藤雄気
  • Organizer: 大阪市立大学セミナー
  • Place of Presentation: 大阪
  • Invited
• [Presentation] ロドプシンの、ロドプシンによる、ロドプシンのための蛋白質科学 (2013)
  • Author(s): 須藤雄気
  • Organizer: 大阪大学蛋白研セミナー
  • Place of Presentation: 大阪
  • Invited
• [Presentation] 光操作技術の基盤となるロドプシン分子の多様性と可能性 (2012)
  • Author(s): 須藤雄気
  • Organizer: 第7回・NIBB バイオイメージングフォーラム
  • Place of Presentation: 基礎生物学研究所
  • Year and Date: 2012-11-26
• [Presentation] 分子からのボトムアップ研究で拓く光細胞・個体操作 (2012)
  • Author(s): 須藤雄気
  • Organizer: 「細胞を創る」研究会5.0
  • Place of Presentation: 東京工業大学
  • Year and Date: 2012-11-21
• [Presentation] Functional diversity of sensory rhodopsins from microbes (2012)
  • Author(s): Sudo, Y
  • Organizer: 15th International Conference on Retinal Proteins
  • Place of Presentation: Ascona, Switzerland
  • Year and Date: 2012-09-30
• [Presentation] 「Simple is the first?」 : 微生物由来レチナール蛋白質の分子進化と多様性 (2012)
  • Author(s): 須藤雄気
  • Organizer: 第14回日本進化学会年会(シンポジウム)
  • Place of Presentation: 八王子
  • Year and Date: 2012-08-21
• [Presentation] 色付きタンパク質の生物学的・化学的・物理学的研究の"面白さ"と"奥深さ" (2012)
  • Author(s): 須藤雄気
  • Organizer: 第1回超異分野学会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-17
• [Presentation] 微生物型ロドプシンタンパク質の利用を考える (2012)
  • Author(s): 須藤雄気
  • Organizer: 平成２３年度「若手研究者による分野間連携研究プロジェクト」NINS program for cross-disciplinary study, 山中＆古谷プロジェクト冬の合同会議(招待講演)
  • Place of Presentation: 箱根
• [Presentation] 色付きタンパク質の生物学的・化学的・物理学的研究の面白さと奥深さ (2012)
  • Author(s): 須藤雄気
  • Organizer: 第1回超異分野学会(招待講演)
  • Place of Presentation: 東京
• [Presentation] Cell and micro-organism manipulations with photo-functional and photo-controllable proteins (2012)
  • Author(s): 須藤雄気
  • Organizer: JST さきがけ研究領域合同国際シンポジウム 「持続する社会を先導する光科学：環境・エネルギー・機能材料」(招待講演)
  • Place of Presentation: 日吉
• [Presentation] Molecular and evolutionary aspects of microbial sensory rhodopsin (2011)
  • Author(s): Sudo, Y. & Homma, M
  • Organizer: 5th Asia and Oceania Conference for Photobiology (AOCP)
  • Place of Presentation: Nara, Japan
• [Presentation] ロドプシンに魅せられて-微生物の光情報伝達と光制御ツール開発- (2011)
  • Author(s): 須藤雄気
  • Organizer: 筑波大学・物質工学セミナー(招待講演)
  • Place of Presentation: 筑波
• [Presentation] Cell and micro-organism manipulations with photo-functional and photo-controllable proteins (2011)
  • Author(s): Sudo, Y.
  • Organizer: AS-JST Joint Workshop on Innovative Use of Light and Nano/Bio(招待講演)
  • Place of Presentation: Taipei, Taiwan
• [Presentation] 7回膜貫通型色素結合タンパク質（ロドプシン）の調製法 (2011)
  • Author(s): 須藤雄気
  • Organizer: 第11回・日本蛋白質科学会年会 蛋白質科学会アーカイブワークショップ(招待講演)
  • Place of Presentation: 大阪
• [Presentation] Type1ロドプシンで初めて見いだされたall-trans→11-cis型光異性化反応 (2011)
  • Author(s): 須藤雄気, 沖津貴志, 水野操, 和田昭盛, 水谷泰久, 本間道夫, 古谷祐詞
  • Organizer: 第38回・生体分子科学討論会
  • Place of Presentation: 筑波
• [Presentation] Molecular and evolutionary aspects of microbial sensory rhodopsin (2011)
  • Author(s): Sudo, Y., Homma, M.
  • Organizer: 5th Asia and Oceania Conference for Photobiology (AOCP)(招待講演)
  • Place of Presentation: Nara, Japan
• [Presentation] 微生物型ロドプシンタンパク質における特異な光反応 (2011)
  • Author(s): 須藤雄気, 本間道夫
  • Organizer: 生理学研究所研究会「作動中の膜機能分子の姿を捉える-静止画から動画へ-」(招待講演)
  • Place of Presentation: 岡崎
• [Presentation] What should we learn from sensory rhodopsins? : Signal transfer mechanism and its application for protein expression (2011)
  • Author(s): 須藤雄気, 本間道夫
  • Organizer: 日本生物物理学会 第49回年会(招待講演)
  • Place of Presentation: 姫路
• [Presentation] Spectral tuning in sensory rhodopsin(センサリーロドプシンの吸収波長制御機構) (2011)
  • Author(s): 須藤雄気, 湯浅恭史, 柴田巡, 鈴木大介, 本間道夫
  • Organizer: 日本生物物理学会 第49回年会
  • Place of Presentation: 姫路
• [Presentation] 新規微生物型ロドプシン(MR)から観る光駆動イオンポンプから光センサーへの分子進化 (2011)
  • Author(s): 須藤雄気, 井原邦夫, 小林詩織, 鈴木大介, 入枝泰樹, 菊川峰志, 神取秀樹, 本間道夫
  • Organizer: 第84回・日本生化学会年会
  • Place of Presentation: 京都
• [Presentation] 光機能性・制御性蛋白質による細胞・個体操作 (2011)
  • Author(s): 須藤雄気
  • Organizer: 第４回文部科学省「最先端の光の創成を目指したネットワーク研究拠点プログラム」シンポジウム(招待講演)
  • Place of Presentation: 名古屋
• [Presentation] 微生物型ロドプシンの基礎的研究とそこから見える新たな地平 (2011)
  • Author(s): 須藤雄気
  • Organizer: 横浜国立大学研究セミナー「細胞膜中機能性生体分子の構造生物学的研究」(招待講演)
  • Place of Presentation: 横浜
• [Presentation] センサリーロドプシンとの出会いと今後の野望 (2011)
  • Author(s): 須藤雄気
  • Organizer: 名古屋工業大学・生物物理化学研究会シンポジウム「光といのち」(招待講演)
  • Place of Presentation: 名古屋
• [Presentation] ロドプシンを愛して：微生物の光情報伝達と光制御ツール開発 (2011)
  • Author(s): 須藤雄気
  • Organizer: 北海道大学電子科学研究所研究セミナー(招待講演)
  • Place of Presentation: 札幌
• [Presentation] Functional diversity of sensory rhodopsins from microbes
  • Author(s): Sudo, Y.
  • Organizer: 15th International Conference on Retinal Proteins
  • Place of Presentation: Monte Verita (Ascona, Switzerland)
  • Invited
• [Presentation] 光操作技術の基盤となるロドプシン分子の多様性と可能性
  • Author(s): 須藤雄気
  • Organizer: 第7回・NIBBバイオイメージングフォーラム
  • Place of Presentation: 自然科学研究機構 基礎生物学研究所（愛知県）
  • Invited
• [Presentation] 分子からのボトムアップ研究で拓く光細胞・個体操作
  • Author(s): 須藤雄気
  • Organizer: 「細胞を創る」研究会5.0
  • Place of Presentation: 東京工業大学（神奈川県）
  • Invited
• [Presentation] 微生物の光利用と光制御ツール開発
  • Author(s): 須藤雄気
  • Organizer: 「次世代の光の利用と物質材料・生命機能」研究会
  • Place of Presentation: 早稲田大学（東京都）
  • Invited
• [Presentation] 「Simple is the first? 」: 微生物由来レチナール蛋白質の分子進化と多様性
  • Author(s): 須藤雄気
  • Organizer: 第14回日本進化学会年会
  • Place of Presentation: 首都大学東京（東京都）
  • Invited
• [Presentation] 微生物型光受容タンパク質の解析と光制御ツール開発
  • Author(s): 須藤雄気
  • Organizer: 第17回日本光生物学協会年会
  • Place of Presentation: 大阪大学（大阪府）
  • Invited
• [Presentation] 光生物物理学-基礎研究のススメ。渇望される薬学部生
  • Author(s): 須藤雄気
  • Organizer: 北海道大学薬学部 第19回「先輩と語る」講演会
  • Place of Presentation: 北海道大学（北海道）
  • Invited
• [Book] "Molecular and evolutionary aspects of microbial sensory rhodopsins" Biochim. Biophys. Acta (2014)
  • Author(s): Inoue, K., Tsukamoto, T., & Sudo, Y
• [Book] Molecular and evolutionary aspects of microbial sensory rhodopsins (2014)
  • Author(s): Inoue, K., Tsukamoto, T., & *Sudo, Y.
  • Total Pages: 16
  • Publisher: Biochim. Biophys. Acta
• [Book] Sensory rhodopsins (2014)
  • Author(s): Tsukamoto, T., & *Sudo, Y.
  • Total Pages: 7
  • Publisher: In: eLS. John Wiley & Sons, Ltd: Chichester.
• [Book] "ロドプシンの波長制御と光情報変換機構" オプトジェネティクス~光工学と遺伝学による行動制御技術の最前線~, 第2章 4節 (2013)
  • Author(s): 須藤雄気
• [Book] オプトジェネティクス-光工学と遺伝学による行動制御技術の最前線-（分担) (2013)
  • Author(s): 須藤雄気
  • Total Pages: 324
  • Publisher: エヌ・ティー・エス
• [Book] "Transport and sensory rhodopsins in microorganisms" CRC Handbook of Organic Photochemistry and Photobiology, the 3rd edition (2012)
  • Author(s): Sudo, Y
  • Publisher: CRC Press, Boca Raton
• [Book] "光受容タンパク質による微生物の光センシングの理解とその利用" 薬学雑誌 (2012)
  • Author(s): 須藤雄気, 本間道夫
• [Book] "高度好塩性微生物の"目" : センサリーロドプシンへの Cl-イオン結合の役割" 極限環境微生物学会誌 (2011)
  • Author(s): 須藤雄気, 井原邦夫, 本間道夫, 加茂直樹
• [Book] 極限環境微生物学会誌 (2011)
  • Author(s): 須藤雄気、井原邦夫、本間道夫、加茂直樹
  • Total Pages: 7
  • Publisher: 高度好塩性微生物の"目"：センサリーロドプシンへのCl-イオン結合の役割
• [Remarks]
  • URL: http://scholar.google.com/citations?hl=en&user=TJ6GZQsAAAAJ&view_op=list_works
• [Remarks]
  • URL: http://profs.provost.nagoya-u.ac.jp/view/html/100001035_ja.html