Neural basis giving rise to speciation in teleost

• Project/Area Number: 23657149
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: University of Toyama (2013); 独立行政法人国立精神・神経医療研究センター (2012); Ehime University (2011)
• Principal Investigator: KAWAGUCHI Masahumi 富山大学, 大学院医学薬学研究部(医学), 助教 (30513056)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 生殖的隔離 / c-fos / 神経活動履歴 / ヨシノボリ / 行動選択 / 生殖前隔離 / 非モデル動物 / 行動生理学 / 生態学 / 神経解剖学 / 神経回路形成

Research Abstract

Reproductive isolation is the elementary process to cause the speciation. However, its forming mechanism is unclear. To elucidate the neural basis of reproductive isolation, we applied the freshwater goby. We detected the expression pattern of c-fos in the brain of male goby, and identified the brain region activated during the courtship behavior for conspecific female or the exclusive behavior against xenogeneic female. Then the difference of neural activity between courtship and exclusive behavior was observed in the neural circuit relating to the visual process, and the pituitary and hypothalamus regulating the systemic reaction. Taken together, it is suggested that the male goby discriminates females depending on the visual perception, and the tectum, the primary input area of vision, is the decision center of behavioral selection.

Research Products

• [Journal Article] Pyrene induces a reduction in midbrain size and abnormal swimming behavior in early-hatched pufferfish larvae (2014)
  • Author(s): Sugahara, Y., Kawaguchi, M., Kurokawa, D., Tosa, Y., Kitamura, S.I., Handoh, I.C., Nakayama, K. and Murakami, Y, Nakayama, K. and Murakami, Y
  • Journal Title: Mar. Pollut. Bull Volume: (掲載決定)
  • Peer Reviewed
• [Journal Article] Developmental epigenetic modification regulates stochastic expression of clustered Protocadherin genes, generating single neuron diversity (2014)
  • Author(s): Toyoda, S., Kawaguchi, M., Kobayashi, T., Tarusawa, E., Toyama, T., Okano, M., Oda, M., Nakauchi, H., Yoshimura, Y., Sanbo, M., Hirabayashi, M., Hirayama, T., Hirabayashi, T., and Yagi, T
  • Journal Title: Neuron Volume: 82 Pages: 94-108
  • Peer Reviewed
• [Journal Article] Pyrene induces a reduction in midbrain size and abnormal swimming behavior in early-hatched pufferfish larvae. (2014)
  • Author(s): Y. Sugahara, M. Kawaguchi, D. Kurokawa, Y. Tosa, S.I. Kitamura, I.C. Handoh, K. Nakayama, Y. Murakami
  • Journal Title: Marine Pollution Bulletin Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Developmental epigenetic modification regulates stochastic expression of clustered protocadherin genes, generating single neuron diversity. (2014)
  • Author(s): Toyoda S, Kawaguchi M, Kobayashi T, Tarusawa E, Toyama T, Okano M, Oda M, Nakauchi H, Yoshimura Y, Sanbo M, Hirabayashi M, Hirayama T, Hirabayashi T, Yagi T.
  • Journal Title: Neuron Volume: 82 Issue: 1 Pages: 94-108
  • DOI: 10.1016/j.neuron.2014.02.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development of the dorsal ramus of the spinal nerve in the mouse embryo : involvement of Semaphorin 3A in dorsal muscle innervation (2013)
  • Author(s): Masuda, T., Taniguchi, M., Sakuma, C., Yamagishi, T., Ueda, S., Kawaguchi, M. and Yaginuma, H
  • Journal Title: Congenit. Anom. (Kyoto) Volume: 53 Pages: 122-126
  • Peer Reviewed
• [Journal Article] Reptiles : a new model for brain evo-devo research (2013)
  • Author(s): Nomura, T., Kawaguchi, M., Ono, K. and Murakami, Y
  • Journal Title: J. Exp. Zool. B Mol. Dev. Evol Volume: 320 Pages: 57-73
  • Peer Reviewed
• [Journal Article] Evolutionary divergence of trigeminal nerve somatotopy in amniotes (2013)
  • Author(s): Rhinn, M., Miyoshi, K., Watanabe, A., Kawaguchi, M., Ito, F., Kuratani, S., Baker, C.V.H., Murakami, Y. and Rijli, F.M
  • Journal Title: J. Comp. Neurol Volume: 521 Pages: 1378-1394
  • Peer Reviewed
• [Journal Article] Development of the dorsal ramus of the spinal nerve in the mouse embryo: involvement of semaphorin 3A in dorsal muscle innervation. (2013)
  • Author(s): T. Masuda, M. Taniguchi, C. Sakuma, T. Yamagishi, S. Ueda, M. Kawaguchi, H. Yaginuma
  • Journal Title: Congenital Anomalies (kyoto) Volume: 53 Issue: 3 Pages: 122-126
  • DOI: 10.1111/cga.12019
  • Peer Reviewed
• [Journal Article] Evolutionary divergence of trigeminal nerve somatotopy in amniotes. (2013)
  • Author(s): Rhinn M, Miyoshi K, Watanabe A, Kawaguchi M, Ito F, Kuratani S, Baker CV, Murakami Y, Rijli FM
  • Journal Title: Journal of Comparative Neurology Volume: 521 Issue: 6 Pages: 1378-1394
  • DOI: 10.1002/cne.23236
  • Peer Reviewed
• [Journal Article] Reptiles: a new model for brain evo-devo research. (2013)
  • Author(s): Nomura T*, Kawaguchi M, Ono K, Murakami Y*
  • Journal Title: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution Volume: 320 Issue: 2 Pages: 57-73
  • DOI: 10.1002/jez.b.22484
  • Peer Reviewed
• [Journal Article] Single-neuron diversity generated by Protocadherin- βcluster in mouse central and peripheral nervous systems (2012)
  • Author(s): Hirano, K., Kaneko, R., Izawa, T., Kawaguchi, M., Kitsukawa, T. and Yagi, T
  • Journal Title: Front. Mol. Neurosci Volume: 5 Pages: 90-99
  • Peer Reviewed
• [Journal Article] Nervous system disruption and concomitant behavioral abnormality in early hatched pufferefish larvae exposed to heavy oil (2012)
  • Author(s): Kawaguchi, M., Sugahara, Y., Watanabe, T., Irie, K., Ishida, M., Kurokawa, D., Kitamura, S.I., Takata, H., Handoh, I.C., Nakayama, K. and Murakami, Y
  • Journal Title: Environ. Sci. Pollut. Res Volume: 19 Pages: 2488-2497
  • Peer Reviewed
• [Journal Article] Nervous system disruption and concomitant behavioral abnormality in early-hatched pufferfish larvae exposed to heavy oil (2012)
  • Author(s): Masahumi Kawaguchi, Yuki Sugahara, Tomoe Watanabe, Kouta Irie, Minoru Ishida, Daisuke Kurokawa, Shin-Ichi Kitamura, Itsuki C. Handoh, Kei Nakayama, Yasunori Murakami.
  • Journal Title: Enviromental Science and Pollution Research Volume: (in press) Issue: 7 Pages: 2488-2497
  • DOI: 10.1007/s11356-012-0833-0
  • Peer Reviewed
• [Journal Article] Single-neuron diversity generated by Protocadherin-β cluster in mouse central and peripheral nervous systems (2012)
  • Author(s): Hirano K
  • Journal Title: Front. Mol. Neurosci. Volume: 5 Pages: 90-90
  • DOI: 10.3389/fnmol.2012.00090
  • Peer Reviewed
• [Journal Article] Disruption of Sema3A expression causes abnormal neural projection in heavy oil exposed Japanese flounder larvae (2011)
  • Author(s): Kawaguchi, M., Song, J.Y., Irie, K., Murakami, Y., Nakayama, K. and Kitamura, S.I
  • Journal Title: Mar. Pollut. Bull Volume: 63 Pages: 356-361
  • Peer Reviewed
• [Journal Article] Effect of heavy oil on the development of the nervous system of floating and sinking teleost eggs (2011)
  • Author(s): Kawaguchi, M., Irie, K., Mizuno, K., Song, J.Y., Nakayama, K., Kitamura, S.I. and Murakami, Y
  • Journal Title: Mar. Pollut. Bull Volume: 63 Pages: 297-302
  • Peer Reviewed
• [Journal Article] Effect of heavy oil on the development of the nervous system of floating and sinking teleost eggs. (2011)
  • Author(s): Kouta Irie*, Masahumi Kawaguchi*, Kaori Mizuno, Jun-Young Song, Kei Nakayama, Shin- Ichi Kitamura, Yasunori Murakami
  • Journal Title: Marine Pollution Bulletin Volume: 63 Issue: 5-12 Pages: 297-302
  • DOI: 10.1016/j.marpolbul.2011.04.018
  • Peer Reviewed
• [Journal Article] Disruption of Sema3A expression causes abnormal neural projection in heavy oil exposed Japanese flounder larvae. (2011)
  • Author(s): Masahumi Kawaguchi*, Jun-Young Song*, Kouta Irie, Yasunori Murakami, Kei Nakayama, Shin-Ichi Kitamura
  • Journal Title: Marine Pollution Bulletin Volume: 63 Issue: 5-12 Pages: 356-361
  • DOI: 10.1016/j.marpolbul.2011.01.022
  • Peer Reviewed
• [Presentation] 求愛と拒絶の神経回路~ヨシノボリの生殖前隔離を制御する神経基盤~ (2014)
  • Author(s): 川口 将史
  • Organizer: 新潟大学 生物学講会~生物学を職業にするという事~
  • Place of Presentation: 新潟大学
  • Year and Date: 2014-05-23
  • Invited
• [Presentation] ヨシノボリ属の求愛行動を制御する神経基盤の解析 (2014)
  • Author(s): 川口 将史
  • Organizer: 東京大学 第211回臨時生物科学セミナー
  • Place of Presentation: 東京大学
  • Year and Date: 2014-03-26
  • Invited
• [Presentation] セマフォリン/プレキシン シグナルは扁桃体と分界条床核との接続を制御する (2014)
  • Author(s): 須藤 文和, 川口 将史, 大隅 典子, 一戸 紀孝
  • Organizer: 第47回日本発生生物学会
  • Place of Presentation: 名古屋大学
• [Presentation] ヨシノボリ属の求愛行動を制御する神経基盤の解析 (2014)
  • Author(s): 川口 将史
  • Organizer: 日本行動神経内分泌研究会
  • Place of Presentation: 秩父
  • Invited
• [Presentation] 求愛と拒絶の神経回路～ヨシノボリの生殖前隔離を制御する神経基盤～ (2014)
  • Author(s): 川口 将史
  • Organizer: 新潟大学セミナー
  • Place of Presentation: 新潟大学 理学部 生物学科
  • Invited
• [Presentation] ヨシノボリ属の求愛行動を制御する神経基盤の解析 (2014)
  • Author(s): 川口 将史
  • Organizer: 東京大学セミナー
  • Place of Presentation: 東京大学 理学部 生物学科
  • Invited
• [Presentation] ヨシノボリ属の求愛行動を制御する神経基盤の解析 (2013)
  • Author(s): 川口 将史, 柴田 淳也, 川西 亮太, 曽我部 篤, 大森 浩二, 一條 裕之, 松本浩司, 村上 安則
  • Organizer: 第84回 日本動物学会
  • Place of Presentation: 岡山大学
• [Presentation] アフリカツメガエルの神経回路形成におけるSlit2 とRobo2 の役割 (2013)
  • Author(s): 塚野 清人, 深川 真惟, 川口 将史, 鈴木 賢一, 新居 由佳子, 高田 裕美, 村上 安則
  • Organizer: 第84回日本動物学会
  • Place of Presentation: 岡山大学
• [Presentation] Dnmt3b 依存的なDNA メチル化は単一神経細胞におけるクラスター型プロトカドヘリン遺伝子群の確率的発現と樹状突起の自己忌避を制御する (2013)
  • Author(s): 豊田 峻輔, 川口 将史, 小林 俊寛, 足澤 悦子, 遠山 知子, 岡野 正樹, 小田昌朗, 中内 啓光, 吉村 由美子, 三宝誠, 平林 真澄, 平山 晃斉, 平林 敬浩, 八木 健
  • Organizer: 第36回日本神経科学会
  • Place of Presentation: 京都国際会議場
• [Presentation] Dnmt3b依存的なDNAメチル化は単一神経細胞におけるクラスター型プロトカドヘリン遺伝子群の確率的発現と樹状突起の自己忌避を制御する (2013)
  • Author(s): 豊田 峻輔, 川口 将史, 小林 俊寛, 足澤 悦子, 遠山 知子, 岡野 正樹, 小田 昌朗, 中内 啓光, 吉村 由美子, 三宝 誠, 平林 真澄, 平山 晃斉, 平林 敬浩, 八木 健
  • Organizer: 第36回日本神経科学学会
  • Place of Presentation: 京都国際会議場
• [Presentation] ヨシノボリ属の求愛行動を制御する神経基盤の解析 (2013)
  • Author(s): 川口 将史, 柴田 淳也, 川西 亮太, 曽我部 篤, 大森 浩二, 一條 裕之, 松本 浩司, 村上 安則
  • Organizer: 第84回日本動物学会
  • Place of Presentation: 岡山大学
• [Presentation] アフリカツメガエルの神経回路形成におけるSlit2とRobo2の役割 (2013)
  • Author(s): 塚野 清人, 深川 真惟, 川口 将史, 鈴木 賢一, 新居 由佳子, 高田 裕美, 村上 安則
  • Organizer: 第84回日本動物学会
  • Place of Presentation: 岡山大学
• [Presentation] カワヨシノボリの求愛行動を制御する神経基盤の解析 (2012)
  • Author(s): 川口 将史, 柴田 淳也, 川西 亮太, 曽我部 篤, 大森 浩二, 松本 浩司, 村上安則
  • Organizer: 第83回日本動物学会
  • Place of Presentation: 大阪大学
• [Presentation] 脊椎動物における小脳神経回路の起源 (2012)
  • Author(s): 川口 将史, 塚野 清人, 猟山 直也, 新居 由佳子, 鈴木 賢一, 高田 裕美, 村上 安則
  • Organizer: 第35回日本神経科学会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] クラスター構造依存的なプロモーターDNA メチル化はプロトカドヘリン遺伝子クラスター内のアイソフォーム発現の分配を制御する (2012)
  • Author(s): 豊田 峻輔, 川口 将史, 遠山 知子, 岡野 正樹, 小田 昌朗, 小林 俊寛, 平山晃斉, 平林 敬浩, 八木 健
  • Organizer: 第36回日本神経科学会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] カワヨシノボリの求愛行動を制御する神経基盤の解析 (2012)
  • Author(s): 川口将史, 柴田淳也, 川西亮太, 曽我部篤, 大森浩二, 松本浩司, 村上安則
  • Organizer: 第83回日本動物学会
  • Place of Presentation: 大阪大学 豊中キャンパス
• [Presentation] クラスター構造依存的なプロモーターDNAメチル化はプロトカドヘリン遺伝子クラスター内のアイソフォーム発現の分配を制御する (2012)
  • Author(s): 豊田峻輔, 川口将史, 遠山知子, 岡野正樹, 小田昌明, 小林俊寛, 平山晃斉, 平林敬浩, 八木健
  • Organizer: 第35回日本神経科学会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 脊椎動物における小脳神経回路の進化 (2011)
  • Author(s): 川口 将史
  • Organizer: 筑波大学下田臨海実験センターセミナー
  • Place of Presentation: 筑波大学下田臨海実験センター
  • Year and Date: 2011-06-09
• [Presentation] Promoter DNA methylation is dependent on gene cluster structure and regulates allocation of isoforms expression in each Protocadherin cluster (2011)
  • Author(s): 豊田 峻輔, 川口 将史, 遠山 知子, 岡野 正樹, 小田 昌朗, 平林 真澄, 平林敬浩, 八木 健
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 脊椎動物における小脳神経回路の起源 (2011)
  • Author(s): 川口 将史, 猟山 直也, 新居 由佳子, 塚野 清人, 高田 裕美, 村上 安則
  • Organizer: 第82回日本動物学会
  • Place of Presentation: 大雪クリスタルホール (旭川)
• [Presentation] Evolution of developmental plan for peripheral nervous system in amniote trunk region (2011)
  • Author(s): 川口 将史, 渡辺 愛己, 真喜屋 宏美, 長島 寛, 川崎 能彦, 平田 たつみ, 増田 知之, 倉谷 滋, 村上 安則
  • Organizer: 第34回日本神経科学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 重油とその構成物質暴露による硬骨魚類の初期発生と行動への影響 (2011)
  • Author(s): 菅原 由貴, 川口 将史, 渡部 友恵, 半藤 逸樹, 北村 真一, 仲山 慶, 村上 安則
  • Organizer: 第17回日本環境毒性学会・バイオアッセイ研究会合同研究発表会
  • Place of Presentation: 鹿児島大学
• [Presentation] Effect of heavy oil exposure during embryogenesis of the nervous system and behavior (2011)
  • Author(s): 川口 将史, 菅原 由貴, 渡部 友恵, 入江 浩太, 北村 真一, 仲山 慶, 半藤 逸樹, 村上 安則
  • Organizer: International Symposium on Advanced Studies by Young Scientists on Environmental Science and Ecotoxicology
  • Place of Presentation: 愛媛大学(Best Presentation Award 受賞)
• [Presentation] Effect of heavy oil and polycyclic aromatic hydrocarbons on developing pufferfish nervous system (2011)
  • Author(s): 村上 安則, 菅原 由貴, 川口 将史, 半藤 逸樹, 北村 真一, 仲山 慶
  • Organizer: 15th international Symposium on Toxicity Assessment
  • Place of Presentation: City University of Hong Kong
• [Presentation] 脊椎動物における小脳神経回路の進化 (2011)
  • Author(s): 川口将史, 猟山直也, 新居由佳子, 塚野清人, 高田裕美, 村上安則
  • Organizer: 第82回日本動物学会年会
  • Place of Presentation: 旭川
• [Presentation] Evolution of developmental plan for peripheral nervous system in amniote trunk region (2011)
  • Author(s): 川口将史, 渡邊愛己, 真喜屋宏美, 長島寛, 川崎能彦, 平田たつみ, 増田知之, 倉谷滋, 村上安則
  • Organizer: 第34回日本神経科学会年会
  • Place of Presentation: 横浜
• [Presentation] Effect of heavy oil exposure during embryogenesis of the nervous system and behavior (2011)
  • Author(s): 川口将史, 菅原由貴, 渡部友恵, 入江浩太, 北村真一, 仲山慶、半藤逸樹, 村上安則
  • Organizer: International Symposium on Advanced Studies by Young Scientists on Environmental Science and Ecotoxicology
  • Place of Presentation: 松山
• [Presentation] Effect of heavy oil and polycyclic aromatic hydrocarbons on the developing pufferfish nervous system (2011)
  • Author(s): 村上安則, 菅原由貴, 川口将史, 半藤逸樹, 北村真一, 仲山慶
  • Organizer: 15th International Symposium on Toxicity Assessment
  • Place of Presentation: Hong Kong
• [Presentation] 重油とその構成物質暴露による硬骨魚類の初期発生と行動への影響 (2011)
  • Author(s): 菅原由貴, 川口将史, 渡部友恵, 北村真一, 半藤逸樹, 仲山慶, 村上安則
  • Organizer: 第17回日本環境毒性学会・バイオアッセイ研究会合同研究発表会
  • Place of Presentation: 鹿児島
• [Presentation] Promoter DNA methylation is dependent on gene cluster structure and regulates allocation of isoforms expression in each Protocadherin cluster (2011)
  • Author(s): 豊田峻輔, 川口将史, 遠山知子, 岡野正樹, 小田昌朗, 平林敬浩, 八木健
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Book] Interdisciplinary Studies on Environmental Chemistry Vol.6 "Advanced Environmental Studies by Young Scientists" Global COE Program, Ehime University (2012)
  • Author(s): Edited by : Kawaguchi, M., Misaki, K., Sato, H., Yokokawa, T., Itai, T., Nguyen, T.M., Ono, J., and Tanabe, S
  • Total Pages: 470
  • Publisher: TERRAPUB 出版
• [Book] Establishment of the protocol for developmental analysis and observation of embryogenesis and axonogenesis in a freshwater goby, Rhinogobius flumineus. In: Interisciplinary Studies on Environmental Chemistry Vol.6 "Advanced Environmental Studies by Young Scientists" (2012)
  • Author(s): Kawaguchi, M., Shibata, J., Kawanishi, R., Sogabe, A., Kawanaka, T., Matsumoto, K., Omori, K. and Murakami, Y
  • Publisher: TERRAPUB 出版
• [Book] Applicability of human hair as a bioindicator for trace elements exposure. In : Interisciplinary Studies on Environmental Chemistry Vol.6 "Advanced Environmental Studies by Young Scientists" (2012)
  • Author(s): Noguchi, T., Itai, T., Kawaguchi, M., Takahashi, S. and Tanabe, S
  • Publisher: TERRAPUB 出版
• [Book] Interdisciplinary Studies on Environmental Chemistry Vol.6 "Advanced Environmental Studies by Young Scientists" (2012)
  • Author(s): Edited by: Masahumi Kawaguchi, Kentaro Misaki, Hiroyuki Sato, Taichi Yokokawa, Takaaki Itai, Tue M. Nguyen, Jun Ono, Shinsuke Tanabe
  • Total Pages: 470
  • Publisher: TERRAPUB
• [Book] Establishment of the protocol for developmental analysis and observation of embryogenesis and axonogenesis in a freshwater goby, Rhinogobius flumineus. (2012)
  • Author(s): Masahumi Kawaguchi, Junya Shibata, Ryota Kawanishi, Atsushi Sogabe, Torao Kawanaka, Koji Matsumoto, Koji Omori, Yasunori Murakami
  • Total Pages: 8
  • Publisher: TERRAPUB
• [Book] Applicability of human hair as a bioindicator for trace elements exposure. (2012)
  • Author(s): Takako Noguchi, Takaaki Itai, Masahumi Kawaguchi, Shin Takahashi, Shinsuke Tanabe
  • Total Pages: 5
  • Publisher: TERRAPUB